Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/25114
標題: 台灣水鹿 GH、KRT5、RPS8及COL1A1 基因單一核苷酸多態性基因型與產茸量性狀相關性
SNP Genotypes of GH, KRT5, RPS8 and COL1A1 Genes Associated with Velvet Antlers Traits in Formosan Sambar
作者: 陳韋君
Chen, Wei-Jyun
關鍵字: SNPs;單一核甘酸多態性;Formosan Sambar;Velvet Antlers Traits;台灣水鹿;產茸量性狀
出版社: 動物科學系所
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摘要: 
台灣水鹿是我國特有亞種,而所產的鹿茸具高度價值。本試驗利用LIS-SSCP (low ionic strength-single strand conformation polymorphism) 與PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) 技術分析台灣水鹿生長激素 (growth hormone)、角蛋白5 (keratin 5)、核醣體蛋白質S8 (ribosomal protein S8) 及第一型膠原蛋白α1 (collagen, type I, alpha 1) 之基因型及其與產茸量之相關性。各基因以軟體設計專一引子對,以PCR增殖出標的DNA片段,利用SSCP分析檢測有差異的部分序列,進一步定序找出此基因序列內的單一核苷酸變異點(SNPs),再利用PCR-RFLP技術或多重式微序列定序 (multiplex minisequencing method) 進行基因型鑑定,最後進行基因型與產茸量間之相關性分析。結果發現 GH 基因: C222T變異點,於水鹿第1-10剪次,CC型個體產茸量有高於CT型與TT型者之趨勢(P < 0.20)。在第1-3剪次,CC型個體產茸量顯著高於CT型與TT型者(P < 0.05) ; G323A變異點,於水鹿第1-3剪次,AA型個體產茸量高於GG型與 GA型,在第4-6剪次,GA型高於GG型者,第7-10 剪次,GA型高於AA型者(P < 0.20)。RPS8基因: C88T變異點,於水鹿第1-3剪次,CT型個體產茸量高於TT型者(P < 0.20)。COL1A1基因: C241T變異點,於水鹿第於1-10剪次,CC型個體產茸量高於TT 型者(P < 0.20)。而在第7-10剪次,CC型個體產茸量有高於CT型者之趨勢(P < 0.20)。上述基因可能為參與鹿茸生長之候選基因,經由增加實驗樣本數與產茸量性狀資料確認,有助於將來鹿隻產茸量性狀育種選拔之參考。

Formosan sambar (Rusa unicolor swinhoei) is endemic subspecies in Taiwan. The price of the velvet antlers is higher than other livestock products. The study was performed to analysis the genotypes in association with velvet antlers production of the GH (growth hormone), KRT5 (keratin 5), RPS8 (ribosomal protein S8), and COL1A1 (collagen, type I, alpha 1) genes by LIS-SSCP and PCR-RFLP. The specific primers were designed by Primer Express software to amplify the target DNA fragments from Formosan sambar genomic DNA by PCR, and followed by SSCP analysis. Single nucleotide polymorphisms (SNPs) were found by sequencing technology, and then processed PCR-RFLP and multiplex minisequencing method for genotyping. Further study was to investigate the correlation between genotypes and performance in Formosan sambar by SAS program. The results showed that, in the SNP C222T of GH gene, the velvet antler weight in 1st-10th saw with CC type seemed to higher than the CT and TT types in Formosan sambar (P < 0.20) , and velvet antler weight in 1st-3rd saw with CC type was significant higher than the CT and TT types (P < 0.05) ; In the SNP G323A, the velvet antler weight in 1st-3rd saw with AA type seemed to higher than the GA and GG types, but in 4th-6th saw with GA type seemed to higher than the GG type, and in 7th-10th saw with GA type seemed to higher than the AA type (P < 0.20). In the SNP C88T of RPS8 gene, the velvet antler weight in 1st-3rd saw with CC type seemed to higher than the TT type (P < 0.20). The velvet antler weight in 1st-10th saw of CC type of COL1A1 gene seemed to higher than the TT type, but in 7th-10th saw, CC type seemed to higher than the CT type (P < 0.20). These genes may involve in the formation of velvet antler, but need increase the sample size to confirm the results. The results may be useful in Formosan sambar breeding.
URI: http://hdl.handle.net/11455/25114
其他識別: U0005-2907201117091700
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