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Effect of ante-mortem stressors on biochemistry characteristics of Taiwan country chicken and broiler
|關鍵字:||Taiwan country chicken;台灣土雞;stress;biochemistry characteristic;緊迫;生化特性||出版社:||畜產學系||摘要:||
The purpose of this study was to investigate the effects of ante-mortem fasting and stress on stress reaction and changes of biochemistry characteristics post-mortem of Taiwan country chicken and broiler. All chickens were divided into four treatments, first, feed withdrawal for 12h and normal slaughter, second, feed withdrawal for 12h and chased for 10min before slaughter, third, feed withdrawal for 0 h and normal slaughter, and forth, withdrawal for 0 h and chased for 10 min before slaughter. Determine items include serum analysis of ante-mortem, bleeding rate, total heme pigment, blood pH, liver pH, activity of crude calpain and cathepsin D, myofibrillar and sarcoplasmic protein solubility, and panel evaluation.
The results showed that ante-mortem chasing resulted in lower blood pH, bleeding rate, and higher glucose, Ca2+, CK, LDH, and ALP level of serum in broiler and Taiwan country chicken. Especially, that had less decrease of blood pH, higher bleeding rate, and lower CK and LDH level of serum in Taiwan country chicken than broiler. Additionally, ante-mortem chasing in broiler resulted in higher total heme pigment by low bleeding degree because of stress and struggle. Liver pH was higher in Taiwan country chicken than broiler. Aspect of protein function, ante-mortem chasing in broiler resulted in higher activity of crude calpain post-mortem, on the contrary, ante-mortem chasing in Taiwan country chicken resulted in lower activity of crude calpain from breast and higher activity of cathepsin D post-mortem at 24h. however, ante-mortem chasing with feed withdrawal had significant higher myofibrillar and sarcoplasmic protein solubility (p<0.05) post-mortem at 15 min, and ante-mortem chasing without feed withdrawal had the lowest protein solubility in Taiwan country chicken. And, sarcoplasmic protein solubility in broiler had the same results as Taiwan country chicken, however, all treatment had no significant difference on myofibrillar protein solubility from broiler. On the whole, protein solubility in Taiwan country chicken was higher than broiler. The result of panel evaluation showed that ante-mortem chasing with feed withdrawal had higher scores of appearance, juiciness, total acceptability, and lower scores of tenderness in broiler. In addition, ante-mortem chasing with feed withdrawal had lower scores of appearance, tenderness, and higher chewiness, and it had no difference on total acceptability in Taiwan country chicken.
These results showed that changes of blood enzyme were more significant and the degree of bleeding was lower in broiler than Taiwan country chicken after ante-mortem chasing. Although ante-mortem chasing made higher calpain activity and protein solubility (with feed withdrawal) in broiler, the scores of tenderness wasn't higher than control treatment. Additionally, the difference among all treatment in broiler were higher than Taiwan country chicken. On the contrary, in Taiwan country chicken, perhaps because of anti-stress ability resulted that glycogen content still high ante-mortem, so that higer lactic acid accumulation, and resulted lower pH of muscle, and effect on meat quality further. However, the difference among all treatment wasn't significant in Taiwan country chicken than broiler. By these result, it is presumable that Taiwan country chicken herhaps has better anti-stress ability than broiler.
Key words: Taiwan country chicken, stress, biochemistry characterist
|Appears in Collections:||動物科學系|
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