Please use this identifier to cite or link to this item:
標題: 扇形文心蘭開花調節與觀賞用試管花貯運
Flowering Regulation and Transportation of in vitro Ornamental Products of Erycina pusilla (L.) N. H. Williams & M. W. Chase
作者: 蔡函育
Tsai, Han-Yu
關鍵字: 扇形文心蘭;Erycina pusilla;開花調節;貯運;試管花;flowering regulation;transportation;in vitro ornamental products
出版社: 國際農學研究所
引用: Agusti, M., V. Almela, and J. Pons. 1992. Effects of girdling on alternate bearing in citrus. J. Hort. Sci. 67(2):203-210. Arditti, J. 1992. Fundamentals of orchid biology. p. 206-213. John Wiley & Sons, New York. Auer, C. A., and D. B. McConnell. 1984. Simulated transit vibration and silver thiosulfate application affect ethylene production and leaf abscission of Beginia and Schefflera. HortScience 19(4):517-519. Barchi, G. L., A. Berardinelli, A. Guarnieri, L. Ragni, C. Totaro Fila. 2002. Damage to loquats by vibration-simulating intra-state transport. Biosyst. Eng. 82(3):305-312. Bernier, G., A. Havelange, C. Houssa, A. Petitjean, and P. Lejeune. 1993. Physiological signals that induce flowering. Plant Cell 5:1147-1155. Bernier, G., J. M. Kinet and R. M. Sachs. 1981. The physiology of flowering. Vol. 1. Control by nutrition and water stress. p. 14-19. C. R. C. Press. Boca Raton, Fla. Bichsel, R. G., T. W. Starman and Y. T. Wang. 2008. Nitrogen, phosphorus, and potassium requirements for optimizing growth and flowering of the nobile Dendrobium as a potted orchid. HortScience 43(2):328-332. Blanchard, M. G. and Runkle, E. S. 2008. Benzyladenine promotes flowering in Doritaenopsis and Phalaenopsis orchids. J. Plant Growth Regul. 27(2):141-150. Bodson, M. and W. H. Outlaw. 1985. Elevation in the sucrose content of the shoot apical meristem of Sinapis alba at floral evocation." Plant physiol. 79(2):420-424. Bulle, A. A. E., G. Slootweg, C. Vonk Noordegraaf. 2000. Effects of vibration during transport on the quality of pot plants. Acta Hort. 518:193-199. Campos, C. F., P.E.A. Souza, J. V. Coelho, and M. B. A. Gloria. 1996. Chemical composition, enzyme activity and effect of enzyme inactivation on flavor quality of green coconut water. J. Food Process. Preserv. 20:487-500. Chang, C., and W. C. Chang. 2003. Cytokinins promotion of flowering in Cymbidium ensifolium var. misericors in vitro. Plant Growth Regul. 39(3):217-221. Chang, C., W. H. Hu, Y. C. Chen, Y. L. Su, and Y. T. Chiu. 2010. In vitro flowering and mating system of Eulophia graminea Lindl. Bot. Stud. 51:357-362. Chang, C. and H. W. Lin. 2007. In vitro flowering of Aerangis hyaloids (Rchb.) Schltr. Seed & Nursery 9(3):27-36. (Chinese with English abstract) Chen, X. Y. 2003. Studies on flowering in vitro of Dendrobium huoshanness. Master Thesis. Dept. Bio., Natl. South China Normal Univ., China. (Chinese with English abstract) Chen, M. Y. 2009. The studies of environmental stresses on the scapes emergence and PP333 and GA3 regulators on the scape length in amaryllis. Master Thesis. Taichung. Dept. Hort., Natl. Ilan Univ., Taiwan. (Chinese with English abstract) Chen, W. S., H. Y. Liu, Z. H. Liu, L. Yang, and W. H. Chen. 1994. Geibberllin and temperature influence carbohydrate content and flowering in Phalaenopsis. Physiol. Plant. 90(2):391-395. Chen, X. Y., M. Q. Xu, P. Zheng, and G. L. Zhao. 2006. Research advances on flowering in vitro of orchid. J. South China Univ. Trop. Agr. 4:27-31. (Chinese with English abstract) Cheng, K. C., R. S. Lin, Y. I. Huang, and C. W. Cheng. 2000. The effects on quality of carnation cut flowers due to simulated transit vibration. J. Agri. Mach. 9(1):1-18. (Chinese with English abstract) Chia, T. F., J. Arditti, M. I. Segeren, and C. S. Hew. 1999. Review: in vitro flowering orchids. Lindleyana 14(2): 60-76. Chiangwijaya, M. 2012. Analysis of quality criteria and mineral nutrient of in vitro Phalaenopsis plantlet. Master Thesis. Taichung. Dept. Hort., Natl. Chung Hsing Univ., Taiwan. Chiu, Y. T. 2011. The studies of pollination, flowering of in vitro plant of Erycina pusilla and intergeneric crosses with Tolumnia cultivars. Master Thesis. Taichung. Dept. Hort., Natl. Chung Hsing Univ., Taiwan. (Chinese with English abstract) Chiu, Y. T. and C. Chang. 2011a. A survey of biology research in Erycina Pusilla. Seed Nursery 13(1):21-30. (Chinese with English abstract) Chiu Y. T. and C. Chang. 2011b. Induction of in vitro flowering in the Phalaenopsis orchid. J. Taiwan Soc. Hort. Sci. 57(1):43-51. Chiu, Y. T. and C. Chang. 2012. Intergeneric crosses between Erycina pusilla and Tolumnia cultivars. J. Taiwan Soc. Hort. Sci. 58(2):117-124. (Chinese with English abstract) Chiu, Y. T., C. S. Lin, and C. Chang. 2011. In vitro fruiting and seed production in Erycina pusilla (L.) N. H. Williams & M. W. Chase. Propag. Ornam. Plant 11:131-136. Chou, M. L., M. C. Shih, M. T. Chan, S. Y. Liao, C. T. Hsu, Y. T. Haung, J. J. Chen, D. C. Liao, F. H. Wu, C. S. Lin. 2013. Global transcriptome analysis and identification of a CONSTANS-like gene family in the orchid Erycina pusilla. Planta 237:1425-1441. Chu, T. M. 1995. Plant and environment stress. National institute for compilation and translation, Taipei, Taiwan. (Chinese with English abstract) Corbesier, L., G. Bernier and C. Perilleux. 2002. C : N ratio increases in the phloem sap during floral transition of the long-day plants Sinapis alba and Arabidopsis thaliana. Plant Cell Physiol. 43(6):684-688. Couey, H. M. 1982. Chilling injury of crops of tropical and subtropical origin. HortScience. 17(2):162-165. Cushman, L. C., Pemberton, H. B., Miller, J. C., and Kelly, J. W. 1998. Interactions of flower stage, cultivar, and shipping temperature and duration affect pot rose performance. HortScience. 33(4): 736-740. Damon, A. and J. V. Mora. 2008. Retrospective spatial analysis of the pollination of two miniature epiphytic orchids with different pollination strategies in a coffee plantation in Soconusco, Chiapas, Mexico. Bot. J. Linn. Soc. 158:448-459. Dielen, V., V. Lecouvet, S. Dupont and J. M. Kinet. 2001. In vitro control of floral transition in tomato (Lycopersicon esculentum Mill.), the model for autonomously flowering plants, using the late flowering uniflora mutant. J. Exp. Bot. 52:715-723. Dickens, C. W. S. and J. van Staden. 1988. The induction and evocation of flowering in vitro. S. Afr. J. Bot. 54(4):325-344. Duan, J. X. and S. Yazawa. 1994. In vitro floral development in ×Doriella Tiny (Doritis pulcherrima×Kingiella philippinensis). Sci. Hortic. 59(3):253-264. Duan, J. X. and S. Yazawa. 1995a. Floral induction and development in Phalaenopsis in vitro. Plant Cell Tissue Organ Cult. 43(1):71-74. Duan, J. X. and S. Yazawa. 1995b. Induction of precocious flowering and seed formation of ×Doriella Tiny (Doritis pulcherrima × Kingiella philippinensis) in vitro and in vivo. Acta Hort. 397:103-110. Faria, D. T., F. N. Rodrigues, L. V. R. Oliveira and C. Muller. 2004. In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations. Hort. Brasileira. 22:780-783. Felix, L. P. and M. Guerra. 1999. Chromosome analysis in Psygmorchis pusilla (L.) Dodson & Dressier: the smallest chromosome number known in Orchidaceae. Caryologia. 52:165-168. Feng, Y. and Q. L. Lin. 2007. Advances in flowing in vitro. Beijing Agr. 36:46-49. (Chinese with English abstract) Ferreira, W. M., G. B. Kerbauy, J. E. Kraus, R. Pescador, and R. M. Suzuki. 2006. Thidiazuron influences the endogenous levels of cytokinins and IAA during the flowering of isolated shoots of Dendrobium. J. Plant Physiol. 163(11):1126-1134. Fischer, D., W. L. Craig, A. E. Watada, W. Douglas, and B. H. Ashby. 1992. Simulated in-transit vibration damage to packaged fresh market grapes and strawberries. Appl. Eng. Agr. 8(3):363-366. Fishier, M., E. E. Goldschmidt, and S. P. Monselise. 1983. Leaf area and fruit size on girdled grapefruit branches. Proc. SE Pecan Grow. Assn. 70:87-93. Halevy, A. H. and A. M. Kofranek. 1976.The prevention of flower bud and leaf abscission in pot roses during simulated transport. J. Amer. Soc. Hort. Sci. 101(6):658-660. Hee, K. H., C. S. Loh, and H. H. Yeoh. 2007. Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Plant Cell Rep. 26(12):2055-2062. Hew, C. S. and F. Y. Lee. 1989. Control of flowering by floral bud removal in x Aranda Christine under tropical field conditions. J. Jpn. Soc. Hortic. Sci. 58:691-695. Hew, C. S. and J. W. Yong. 2004. Physiology of tropical orchids in relation to the industry. World Scientific, Singapore. Hinsch, R. T., D. C. Slaughter, W. L. Craig, and J. F. Thompson. 1993. Vibration of fresh fruits and vegetables during refrigerated truck transport. Transactions of the ASAE. 36(4):1039-1042. Hou, J. Y., T. L. Setter, and Y. C. A. Chang. 2010. Effects of simulated dark shipping on photosynthetic status and post-shipping performance in Phalaenopsis Sogo Yukidian ‘V3’. J. Amer. Soc. Hort. Sci. 135(2):183-190. Huang, S. H., C. H. Huang, T. M. Shen, and K. L. Huang. 2010a. Differences in the growth and flowering performance of Phalaenopsis having flower stalks of various lengths and being kept in simulated shipping for various durations. J. Taiwan Soc. Hort. Sci. 56(3):193-207. (Chinese with English abstract) Huang, C. C., H. S. Huang, C. Y. Tsai and C. H. Yao. 2010b. The effect of LED lightening during transportation on the quality of Phalaenopsis plants with spike and the effect of transportation temperature on the quality of Dendrobium plants. Special publication of TARI. 154:155-172. (Chinese with English abstract) Huang, C. C., H. S. Huang,C. Y. Tsai, and C. H. Yao. 2011a. The application and improvement of the marine transportation technology for Phalaenopsis plants. Special publication of TARI. 164:9-23. (Chinese with English abstract) Huang, C. C., H. S. Huang, C. Y. Tsai, C. H. Yiau, and I. Ting. 2012. Effects of storage temperature and relative humidity on leaf and flower quality in Dendrobium Lucky Girl. J. Taiwan Soc. Hort. Sci. 58(4):305-314. (Chinese with English abstract) Huang, C. C., S. C. Yu, C. Y. Tsai, H. S. Huang, and G. P. Chang. 2011b. Effect of fertilization after simulated export transportation on flower quality and leaf color of Phalaenopsis Sogo Yukidian ‘V3’. J. Taiwan Soc. Hort. Sci. 57(2):133-142. (Chinese with English abstract) Huang, Q., Z. X. Lai, A. Y. Luan, and S. I. Huang. 2007. Production and applications of tube flowers. Subtrop. Agr. Res. 3(4):300-304. (Chinese with English abstract) Jackson, J. C., A. Gordon, G. Wizzard, K. McCook, and R. Rolle. 2004. Changes in chemical composition of coconut (Cocos nucifera) water during maturation of the fruit. J. Sci. Food. Agr. 84:1049-1052. Juang J. H. and N. Lee. 1986. Effect of activated charcoal, sucrose, and mineral concentration on seed germination and seedling growth of Pleione formosana. J. Chinese Soc. Hort. Sci. 32:61-69. (Chinese with English abstract) Kays, S. J. 1991. Postharvest physiology and handling of perishable plant products. p. 335-408. Van Nostrand Reinhold Inc. New York. Kerbauy, B. B. 1984. In vitro flowering of Oncidium varicosum mericlones (Orchidaceae). Plant Sci. Lett. 35:73-75. Ket, N.V., E.J. Hahn, S.Y. Park, D. Chakrabarty, and K.Y. Peak. 2004. Micropropagation of an endangered orchid Anoectochilus formosanus. Biol. Plant. 48:339-344. Klebs, G. 1913. Uber das Verhaltnis der Aubenwelt zur Entwicklung der Pflanze. Sitz-Ber. Akad. Wiss. Heidelberg Ser. B 5:3-47. Kostenyuk, I., B. J. Oh and I. S. So. 1999. Induction of early flowering in Cymbidium niveo-marginatum Mak in vitro. Plant Cell Rep. 19(1):1-5. Kundon. L. 1930. Flowering production by orchids grown nonsymbiotically. Bot. Gaz. 89:192-199. Lee, C.H. and N. Lee. 1991. Characteristics of morphology and anatomy in root and leaf of Phalaenopsis amabilis. J. Chinese Soc. Hort. Sci. 37:237-248. (Chinese with English abstract) Levy, Y. Y. and Dean, C. 1998. The transition to flowering. Plant Cell 10(12):1973-1989. Lin, L. H. 2006. Effect of growing medium on growth and transport of Phalaenopsis. Natl. Chung Hsing Univ., Dept. Hort., Taiwan, Master Thesis. Taichung. (Chinese with English abstract) Liu, K. T., Y. T. Chiu, and C. Chang. 2011. Intergeneric cross between Erycina pusilla and Oncidiinae species and cultivars. Seed Nursery. 13(1):31-39. (Chinese with English abstract) Lin, W. J., C. L. Chen, C. Chang, and M. J. Tseng. 2012. Transforming fibrillin gene into Erycina pusilla. Horticul. NCHU 37(4):37-51. (Chinese with English abstract) Lin, C. S., C.T. Hsu, H.C. Lu, M. L. Chou, D. C. Liao, Y. T. Huang, Y. Y. Yeh, S. B. Chang, S. C. Shen, F. H. Wu, J. J. W. Chen, M. C. Shih, M. T. Chan. 2013. Catalog of Erycina pusilla miRNA and categorization of reproductive phase-related miRNAs and their target gene families. Plant Mol. Biol. 82:193-204. Livingston, R. B. 1962. Oncidium pusillum—Blooming in flask! Am. Orchid Soc. Bull. 31:1007. Lopez, R. G. and E. S. Runkle. 2005. Environmental physiology of growth and flowering of orchids. HortScience 40(7): 1969-1973. Lu, C. C. 1992. Effects of ethylene, media and physical wounding on tissue culture of Phalaenopsis. Natl. Taiwan Univ., Dept. Hort., Taiwan, Master Thesis. Taipei. (Chinese with English abstract) Luo, W. G. 2006. Effect of temperature or chemical pretreatment on physiology and morphology of simulated transport of Colmanara Wildcat. Natl. Chung Hsing Univ., Dept. Hort., Taiwan, Master Thesis. Taichung. (Chinese with English abstract) McConnell, D. B. and T. J. Sheehan. 1978. Anatomical aspects of chilling injury to leaves of Phalaenopsis Bl. HortScience 13:705-706. Morris, L. L. 1982. Chilling injury of horticultural crops: an overview. HortScience 17 (2):161-162. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue culture. Physiol. Plant. 15:473-497. Nell, T. A., Leonard, R. T., De Hertogh, A. A., and Barrett, J. E. 1995. Postproduction evaluations of potted Asiatic and Oriental hybrid lilies. HortScience. 30(7):1433-1435. Pan, I. C., D. C. Liao, F. H. Wu, H. Daniell, N. D. Singh, C. Chang, M. C. Shih, M. T. Chan, and C. S. Lin. 2012. Complete chloroplast genome sequence of an orchid model plant candidate: Erycina pusilla apply in tropical Oncidium breeding. PlOS one. 7(4):1-12. Peleg, k., and S. Hinga. 1986. Simulation of vibration damage in produce transportation. Transactions of the ASAE. 29(2):633-641. Poole, R. T., C. A. Conover, and J. Ben-Jaacov. 1984. Long-term storage of foliage plants.Sci. Hort. 24:331-335. Sakanishi, Y., H. Imanishi, and G. Ishida. 1980. Effect of temperature on growth and flowering of Phalaenopsis amabilis. Bull. Univ. Osaka Pref. Ser. B. 32:1-9. Šestak, Z. 1985. Photosynthesis during leaf development. p.268-269. Dr. W. Junk, Dordrecht. Sheehan, T. and M. Sheehan. 1989. Orchid genera illustrated 127-Psygmorchis. Amer. Orchid Soc. Bull. 58:24-25. Sim, G. E., C. J. Goh, and C. S. Loh. 2008. Induction of in vitro flowering in Dendrobium Madame Thong-In (Orchidaceae) seedlings is associated with increase in endogenous N6-(Δ2-isopentenyl)-adenine (iP) and N6-(Δ2-isopentenyl)-adenosine (iPA) levels. Plant Cell Rep. 27(8):1281-1289. Sim, G. E., C. S. Loh, and C. J. Goh. 2007. High frequency early in vitro flowering of Dendrobium Madame Thong-In (Orchidaceae). Plant Cell Rep. 26(4):383-393. Simmonds, J. 1982. In vitro flowering on leaf explants of Streptocarpus nobilis. The influence of culture medium components on vegetative and reproductive development. Can. J. Bot. 60(8):1461-1468. Slaughter, D. C., R. T. Hinsch, and J. F. Thompson. 1993. Assessment of vibration injury to Bartlett pears. Transactions of the ASAE. 36(4):1043-1047. Slaughter, D. C., J. F. Thompson, and R. T. Hinsch. 1998. Packaging bartlett pears In polyethylene film bags to reduce vibration injury in transit. Amer. Soc. Agri. Eng. 41(1): 107-114. Smeekens, S. 2000. Sugar-induced signal transduction in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51(1):49-81. So, I. S., K. H. Cho and C. W. Lee. 2003. In vitro flowering in Cymbidium goeringii as affected by putative flower induction treatment. J. Kor. Flower Res. Soc. 11:191-198. Tee, C. S., M. Maziah, and C. S. Tan. 2008. Induction of in vitro flowering in the orchid Dendrobium Sonia 17. Biologia Plantarum 52(4):723-726. Than, M. M. M., A. Pal, and S. Jha. 2009. In vitro flowering and propagation of Bulbophyllum auricomum Lindl., the royal flower of Myanmar. Acta Hort. 829:105-111. Tijskens, L. M. M., M. Sloof, E. C. Wilkinson, and W. G. van Doom. 1996. A model of the effects of temperature and time on the acceptability of potted plants stored in darkness. Postharvest Biol. Technol. 8:293-305. Tsai, M. F. 2009. Influence of shading and girdling on flowering and C/N ratio of wax apple (Syrygiun samaragense Merr. er Perry) Master Thesis. Pingtung. Dept. Plant Ind., Natl. Pingtung Univ., Taiwan. (Chinese with English abstract) Vacin, E. F. and F. W. Went. 1949. Some pH changes in nutrient solutions. Bot. Gaz. 110:605-617. Vaz, A. P. A., R. D. C. L. Figueiredo-Ribeiro, and G. B. Kerbauy. 2004. Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid. Plant Physiol. Biochem. 42:411-415. Vaz, A. P. A., and G. B. Kerbauy. 2000. Effects of mineral nutrients on in vitro growth and flower formation of Psygmorchis pusilla (Orchidaceae). Acta Hort. 520:149-156. Vaz, A. P. A., and G. B. Kerbauy. 2008. In vitro flowering studies in Psygmorchis pusilla. Glob. Sci. 42:427-432. Vergano, P. J., R. F. Testin, W. C. Newall Jr. 1991. Peach bruising: susceptibility to impact, vibration, and compression abuse. Transactions of the ASAE. 34(5):2110-2116. Villiers, H. D., J. G. M. Cutting, G. Jacobs, and D. K. Strydom. 1990. The effect of girdling on fruit growth and internal quality of ''Culemborg'' peach. J. Hort.Sci. 65(2):151-155. Wada, K. and Y. Shinozaki. 1985. Flowering response in relation to C and N Contents of Pharbitis nil plants cultured in nitrogen-poor media. Plant Cell Physiol. 26(3):525-535. Wang, X. 1988. Tissue culture of Cymbidium: plant and flower induction in vitro. Lindleyana 3(4):184-189. Wang, C. Y. 1990. Chilling injury of horticulture crops. CRC Press Llc. Boca Raton, Florida. Wang, C. Y. 1994. Chilling injury of tropical horticultural commodities. HortScience 29(9):986-987. Wang, Y. T. 1995. Phalaenopsis orchid light requirement during the induction of spiking. HortScience 30(1): 59-61. Wang, L. 2004. Studies on in vitro flowering of Dendrobium nobile Lindl. Master Thesis. Dept. Bio., Natl. South China Normal Univ., China. (Chinese with English abstract) Wang, Z. H. 2005. Studies on in vitro flowering of Dendrobium nobile Lindl and Dendrobium moniliforme. Master Thesis. Dept. Bio., Natl. South China Normal Univ., China. (Chinese with English abstract) Wang, Y. T. 2007. Temperature, duration in simulated shipping, and thermal acclimatization on the development of chilling injury and subsequent flowering of Phalaenopsis. J. Amer. Soc. Hort. Sci. 132(2):202-207. Wang, X. J. C. Chen, G. Y. Liu, M. X. Gu, and C. H. Bao. 1981. Clonal propagation of orchids by means of tissue culture. Acta Phytophysiol. Sinica. 7(2):203-207. (Chinese with English abstract) Wang, Y. X., Y. M. Chen, and T. M. Shen. 2006a. Effects of shipping temperature and durations on post-shipping growth of sphagnum moss grown Phalaenopsis. J. Taiwan Soc. Hort. Sci. 52:311-320. (Chinese with English abstract) Wang G. Y., P. Liu, Z. H. Xu, and N. H. Cai. 1995. Effect of ABA on the in vitro induction of floral buds of Dendrobium candidum Wall. Ex Lindl. Acta Bot. Sinica 37(5):374-378. (Chinese with English abstract) Wang, Z. H., H. Y. Tu, and Q. S. Ye. 2006b. Rapid propagation and in vitro flowering of Dendrobium moniliforme (L.) Sw. Plant Physiol. Commu. 42(6):1143-1144. (Chinese with English abstract) Wang, Z. H., L. Wang, and Q. S. Ye. 2009. High frequency early flowering from in vitro seedlings of Dendrobium nobile. Sci. Hortic. 122(2):328-331. Wang, G. Y., X. U. Zhihong, T. F. Chia, and N. H. Chua. 1997. In vitro flowering of Dendrobium candidum. Sci. China 40:35-42. Washington, D. C. and W. Horwitz. 1970. Official methods of analysis of the Association of Official Analytical Chemists. Association of Official Analytical Chemists. Wen, Y. F., R. L. Lu, and Z. L. Xie. 1999. Rapid propagation and induction of floral buds of Dendrobium huoshanase. Plant Physiol. Commu. 35(4):296-297. (Chinese with English abstract) Williams, N. H., M. W. Chase, T. Fulcher, and W. M. Whitten. 2001. Molecular systematics of the Oncidiinae based on evidence from four DNA regions: expanded circumscriptions of Cyrtochilum, Erycina, Otoglossum and Trichocentrum and a new genus (Orchidaceae). Lindleyana 16:113-139. Withner, C. L. 1959. The orchids: a scientific survey. p.349-350. The Ronald Press Company, New York. Wu, Z. H. 2012. Effect of storage temperature, 1-methylcycopropene and sprayed benzyladenine on nobile type Dendrobium flower quality after simulated transportation. Natl. Chung Hsing Univ., Dept. Hort., Taiwan, Master Thesis. Taichung. Yoshida, S., D. Forno, J. Cock, and K. Gomez. 1976. Determination of sugar and starch in plant tissue. Laboratory manual for physiological studies of rice. The International Rice Research Institute, Philippines. p.46-49. Yu, H. and C. J. Goh. 2000. Differential gene expression during floral transition in an orchid hybrid Dendrobium Madame Thong-In. Plant Cell Rep. 19(9):926-931. Zhang, H., Z. C. Bai, S. J. Liu, D. F. Ma, Y. Liu, and H. Liu. 2009. Research status and prospect of flowering plants in vitro. J. Anhui Agr. Sci. 37(17):7958- 7960. (Chinese with English abstract) Zhang, N. G., T. W. H. Yong, C. S. Hew, and X. Zhou. 1995. The production of cytokinin, abscisic acid and auxin by CAM orchid aerial roots. J. Plant. Physiol. 147(3):371-377. Zheng, L. M. and J. L. Pang. 2006. In vitro flowering of culture from a hybrid of Cymbidium goeringii and C. hybridium. J. Plant Physiol. Molec. Bio. 32(3):320-324. (Chinese with English abstract) Zhu, G. B., B. Y. Yang, and A. Y. Ao. 2008. Tissue culture and in vitro flowering of Cymbidium kanran Makino. Plant Physiol. Commu. 44(3):513-514. (Chinese with English abstract)
本試驗中調節開花的方法有植株刻傷、植株平放於培養基上、以及改變培養基中無機氮和蔗糖之濃度。植株刻傷處理及植株刻傷與平放處理可降低植株體內的全氮含量,並增加澱粉及全醣含量,因此可提高植株體內之碳氮比。植株碳氮比提高,花梗生成數在14週內增加。在植株生長方面,植株刻傷會產生較多褐化葉、褐化根、以及較重的鮮重。降低培養基無機氮含量至原來的1/16 (118.75 mg L-1 KNO3 and 103.125 mg L-1 NH4NO3),植株可產生較多的花苞與開花數,並對植株生長沒有負面的影響。此外,增加培養基中蔗糖的含量至40 g L-1,植株也可生成較多的花苞與開花數,但提升蔗糖含量也會使植株鮮重增加以及葉片增厚。

Erycina pusilla is a miniature orchid with obvious yellow flower and can constantly produce flowers within in vitro condition. Thus, Erycina pusilla is considered as a suitable orchid to develop in vitro ornamental products. In this study, flowering regulation and transportation was examined in in vitro Erycina pusilla.
Physical wounding on plants, modifying the direction of inserting plants into the medium, modulating the composition of inorganic nitrogen and sucrose concentration in the culture medium were conducted in regulating in vitro Erycina pusilla flowering. The results showed that performing physical injury alone and combination treatment of physical injury and different placement led to lower inorganic nitrogen content, higher starch and total carbohydrate content. Thus, the C/N ratio of plants was regulated to higher value, and more flower stalks production were observed after 14 weeks of culture. In the part of plant condition, performing mechanical injury led to more browning roots and leaves, and heavier plant weight. Modulating the concentration of inorganic nitrogen to 1/16 (118.75 mg L-1 KNO3 and 103.125 mg L-1 NH4NO3) in culture medium promoted the production of flower bud and flower, fewer abortive flower buds, and no severe damage was detected on plant growth. More flower bud and flower production were observed when increasing sucrose concentration to 40 g L-1 in medium. However, thicker leaves were observed in treatment of increasing sucrose concentration from 40 to 60 g L-1.
Both simulated transportation and practical transportation were conducted in in vitro ornamental products of Erycina pusilla. The simulated transportation of in vitro Erycina pusilla lasted under the range of 15-30℃ and darkness for a week without losing its quality. For the simulated transportation in darkness for 4 weeks, the suitable storage temperature is 20-25℃. More abortive flower buds resulting from 4-week dark treatment in 15-30℃was recorded. In addition, severe browning leaves and browning flower stalks were observed on plants with 4-week darkness at 30℃. As for the practical transportation, severe damage of broken culture medium and plant displacement from shipment handling and transportation resulted in no intact ornamental products. Moreover, the low temperature around 10℃ during winter caused serious deterioration of browning and flower production. More factors such as preventing serious vibration, contamination problem, unknown environmental change, and Customs quarantine should be considered to avoid the loss in practical transportation.
According to these experiments, flower regulation and transportation for 1 to 4 weeks could be applied on in vitro Erycina pusilla. These finding showed that Erycina pusilla is an ideal plant material for developing in vitro ornamental product of orchid.
其他識別: U0005-1208201314461300
Appears in Collections:國際農學研究所

Files in This Item:
File Description SizeFormat Existing users please Login
nchu-102-7100030754-1.pdf1.91 MBAdobe PDFThis file is only available in the university internal network    Request a copy
Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.