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標題: 酚類化合物之生物分解
Biodegradation of Phenolic Compounds
作者: 吳俊德
Wu, Chun Teh
關鍵字: Allelopathy;植物相剋作用;phenolic compounds;酚類化合物
出版社: 土壤學系
作用的角色,台灣蔗田土壤中含有ferulic acid、p-hydroxybenzoic acid
、p-coumaric acid、vanillic acid、syringic acid 等酚酸引起蔗作減
產現象。 此外台灣二期稻作減產及蘆筍減產,其缺株率高達40%證實
為3,4-dihydroxybenzoic acid等酚酸所引起,然而甚少有從微生物的觀點
著手,解決土壤中酚類化合物所引起的問題。 本研究的目的即在篩選具有
分解酚酸能力的細菌來解決土壤酚酸所引起的植物相剋作用。 本實驗分
別以 ferulic acid 及p-hydroxybenzoic acid 為唯一碳源所篩選出來的
分解菌,各別為486f、486p菌種;486f菌對於ferulic acid 100 ppm之分解
p-hydroxybenzoic acid 100 ppm之分解能力第7天殘餘百分率為51%(分解
率為49%),第12天總移出率為67%。 以Pseudomonas putida作為已知菌種
putida對p-hydroxybenzoic acid之分解率已達100%,而486p之分解率僅
為49%(殘餘百分率為51%),顯然,Pseudomonas putida其分解能力大於486p
。而486f對於ferulic acid之解第7天其殘餘百分率為21%,而Pseudomonas
putida為48%,由結果所示486f其分解能力大於Pseudomonas putida。 在
酚酸的代謝實驗中以50, 100, 1000 ppm不同的濃度作為觀察,發現低濃度
下其代謝速率快,且完全分解;高濃度將造成抑制作用,而影響分解作用 在
與土壤微生物之協同代謝作用之結果。 胞內酚酸測定之實驗結果顯示兩
acid之中間代謝產物的存在。酚酸毒性試驗的結果顯示,200 ppm的
ferulic acid已完全抑制萵苣的生長,而p-hydroxybenzoic acid其抑制率

The products of botanical serial metabolism, phenolic
compounds for instance, often play a role of allelopathy in the
nature. The soil of sugar cane field in Taiwan contains phenolic
acids, such as ferulic acid, P-hydroxybenzoic acid, p-coumaric
acid, vanillic acid, syringic acid which cause decrease in sugar
cane production. Besides, it is verified that decreases in the
production of semiannual rice crops and asparagus whose rate of
null plants reach as heigh as 40%, are caused by phenolic
compounds, 3,4-dihydroxybenzoic acid, through few research treat
these problems induced by phenolic compounds in the soil from
micro-biological points of view. The purpose of this
research is to solve the problem of allelopathy brought about by
phenolic acids existing in the soil by sifting out bacteria that
are capable of resolving phenolic acids. This experiment
separately employs resolving bacteria, species of 486f and 486p
that are shifted out by way of using ferulic acid and p-
hydroxbenzoic acid as the sole carbon source. The total removal
rate of the resolving capacity of Bacterium 486f against ferulic
acid 100ppm on the 10th day is 86%, on the 3rd the remaining
percentage is 45% (resolving rate 55%); the resolving capacity
of Bacterium 486p against p-hydroxybenzoic acid 100 ppm has the
remaining percentage of 51% on 7th day (resolving rate 49%), and
the total removal rate is 67%. The resolving rate of
Pseudomonas putida is compared separately with those 486f ande
486p, the afore-mentioned species of bacteria. The experiment
inidicates the resolving rate of Pseudomonas putida against p-
hydroxybenzoic acid on 7th day reaches 100%, while that of 486p
is only 49% (remaining percentage is 51%). The resolving
capability of Pseudomonas putida is evidently larger than that
of 486p. The resolving power of 486f against ferulic acid shows
its remaining percentage at 21% on the 7th day while the
Pseudomonas putida is 48% from the results it is known that
Pseudomonas putida. In the experiment of phenolic acid
metabolism, different densities of 50, 100, and 1000 ppm are
separately observed, finding that not only the speed of
metabolism is fast at the low density but the resolution is
complete; while at the height density the function is refrained
and the resolving affected. In the exsperiment using glucose as
foreign carbon source to test influence of phenolic acid
resolution function,the result shows that the resolving bacteria
can make use of glucose as foreign carbon source and accelerate
the resolution, through incomplete; then, if added with soil
solution, it does not only accelerates the resolution faster but
also does the work perfectly. The rate of resolving speeds is
evidently superior when glucose is added in, which is inferred
to be the consequence of synergistic function between resolving
bacteria and soil microorganisms. The result of experiment
of endocytic phenolic acid measurement apparently indicates that
both the resolving bacteria have endocytic phenolic acid, and in
the measurement of 486f endocytic phenolic acid, existence of
mid-metabolic procuct of vanillic acid was found. The result
of phenolic acid toxicity test indicates that ferulic acid
completely suppress growth of lettuce at 200 ppm, and the rate
of suppression of p-hydroxybenzoic acid only reaches 44.4%,
telling us that the toxicity of different plant toxins differ.
The result of the experiment of biological analysis shows that
the lettuce gets improvement on the first day it is planted
after receiving the bacteria treatment, which is conjectured to
be the result of bacteria's fast resolving, for the organic
carbon contained in Erhshui Soil is high. Therefore, the
relation between the nature of soil and the microorganisms
influence the activities of the microorganisms.
Appears in Collections:土壤環境科學系

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