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標題: 不同施肥處理對土壤微生物多樣性之探討
Effect of fertilization on soil microbial diversity
作者: 廖彩汝
關鍵字: 堆肥;土壤微生物;生物多樣性
出版社: 土壤環境科學系
摘 要
土壤生物多樣性與土壤永續利用有極密切之關係。生物多樣性包含以下互有交集的生物多樣性-分類(taxonomic)多樣性、功能(functional)多樣性及基因(genetic)多樣性。本研究從功能性這方面來探討土壤微生物之多樣性。功能多樣性方面之一是從微生物對碳源代謝的角度,以 Biolog microplates 測試土壤微生物對於不同碳源利用的程度差異,獲得不同的土壤微生物群社對不同碳源代謝的情形。
本研究試驗地為農業委員會農業試驗所試驗所試驗田,屬砂頁岩非石灰性新沖積土,七塊厝系(TCt),自1995年秋做玉米起至2001年春做水稻,已執行了六年的水旱田輪作,設七處理,分別施用豆科綠肥(田菁、埃及三葉草)、廢棄物堆肥(豬糞堆肥)、不易分解之有機質(泥炭)及傳統化肥等。本研究藉由Biolog microplates之方法探討有機肥料與化學肥料的施用對土壤微生物多樣性的影響。

Effect of Fertilization on Soil Microbial Diversity
Soil biodiversity is important to the sustainable utilization of soils. The total biodiversity of an ecosystem includes taxonomic, functional and genetic biodiversities. The purpose of this thesis was to investigate the effect of organic and chemical fertilization on the soil microbial diversity by using Biolg microplate method.
The experimental site was located at Agricultural Research Institute of Council of Agriculture, Taiwan. The test area is a non-calcareous alluvial soil formed from a sandstone and shale. The crop system has being continuously rotated with corn and rice for six years. The treatments included legume green manure, pig waste compost, peat and chemical fertilizer.
The results obtained shows that the metabolic potential on various carbon sources of the microbial community in corn soils was higher than that in paddy rice soils because the aerobic condition in corn soils favored the microorganisms to utilize the carbon sources in Biolog microplate. The number of carbon sources that could be utilized was higher in corn soils than in rice soils. The microbial community in compost, compost + 1/3 (or 2/3) urea (for corn) or ammonium sulfate (for rice), peat or green manure treated soils were relatively similar. The similarity among those were higher than 0.9. However the microbial community in rice soils were less similar than that in corn soils. The statistic analysis shows that the principal components affecting the soil microbial community were crop species and water management. For corn and rice respectively, organic fertilizer was the major factor affecting the structure of soil microbial community.
Key words: Soil, Microbial diversity, Organic fertilizer, Biodiversity.
Appears in Collections:土壤環境科學系

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