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A novel glucose biosensor based on a gold nano-trees modified electrode
|關鍵字:||奈米金三維結構;three-dimensional gold nanoparticle;電化學;葡萄糖檢測;三極式拋棄電極;electrochemical impedance spectroscopy;glucose testing;disposable three-electrode chip||出版社:||機械工程學系所||引用:|| D. Muller, “Oxidation von Glukose mit Extrakten aus Aspegillus niger,” Biochem Z, vol. 199, pp. 136–170, 1928.  L. C. Clark and C. Lyons, “Electrode system for continuous monitoring in cardiovascular surgery,” Ann. NY Acad. Sci., vol. 148, pp. 133-135, 1962.  T. V. Anh, D. Pijanowska, W. Olthuis and P. Bergveld, “Highly sensitive glucose sensor based on work function changes measured by an MOSFET,” Analyst, vol. 128, pp. 1062-1066, 2003.  Y. Lin, F. Lu, Y. Tu and Z. Ren, “Glucose Biosensors Based on Carbon Nanotube Nanoelectrode Ensembles,” Nano Letters, vol. 4 (2), pp 191–195, 2004  S. Zhang, N. Wang, Y. Niu and C. Sun, "Immobilization of glucose oxidase on gold nanoparticles modified Au electrode for the construction of biosensor," Sensors and Actuators B: Chemical, vol. 109, pp. 367-374, 2005.  C. Qiu, X. 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本研究成功的在平面金電極上藉由電化學的方式沉積出具有樹枝狀奈米結構的薄膜，為了簡化製程，本研究進一步以市售之三極式拋棄電極為基材沉積奈米金仙人掌球電極，並藉由共價鍵結合(Covalent Bonding Method)的方式在奈米金仙人掌球電極上被覆一層葡萄糖氧化酵素(Glucose Oxidase，GOD)，形成葡萄糖生物感測器，再以循環伏安法(CV)檢測溶液中葡萄糖的濃度。由於三維奈米金結構具有高生物相容性以及更大的表面積增加電子傳遞之效能，因此本研究之葡萄糖感測器具有低檢測電壓(0.06V)、高靈敏度(2.28 μA/mM．cm2)、迅速反應(1.8s)及與市售系統相容性佳等特點。
The study has successfully developed a thin film with branch-shaped nanostructure on flat gold electrode via electrochemical impedance spectroscopy (ESI). In order to facilitate our fabrication, a commercial disposable three-electrode chip is used to be a substrate to deposit gold nanoparticles. Moreover, Glucose oxidase (GOD) is coated on the selected electrode by covalent bonding method. After the fabrication, the glucose biosensor can measure the concentration of glucose by using cyclic voltammetry (CV). In addition, three-dimensional gold nanoparticles can represent high biocompatibility and larger surface area of reaction zone to increase the efficiency of conveying electrons. As a result, the glucose biosensor has several main advantages such as low requirement of voltage (0.06V), high sensibility (2.28μA/mM．cm2), quick response (1.8 sec) and its potential market.
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