Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/2814
標題: 利用梳狀微流道分離捕捉單一顆粒之實驗研究
Separation and Trapping of Single particles in Comb-like Microchannels
作者: 蔡惟亘
Tsai, Wei-Hsuan
關鍵字: 液動力分離;hydrodynamic filtration;單一顆粒;阻抗;微流道;single particle;impedance;microfluidics
出版社: 機械工程學系所
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摘要: 
本研究利用液動力分離原理,設計梳狀流道結構,用以分離及捕捉單一微顆粒,並結合微電極量測捕捉區間內的阻抗。微流道結構以微影及軟微影製程製作,電極晶片則利用微影製程及Lift-off技術完成,兩者結合而成為本實驗研究之單一顆粒阻抗量測晶片。實驗結果證實,本分離捕捉單一顆粒系統之原理與設計的可行性,並於深50 μm×寬50 μm及深30 μm×寬50 μm之捕捉槽成功分離捕捉直徑20 μm的單一顆粒。另外,於捕捉槽的阻抗量測,可以明確辨別直徑20 μm之聚苯乙烯微顆粒,捕獲前後阻抗值的差異,約增加10%。希望此研究方法與相關設計可以應用於大量檢測單一個體的生醫分析系統。

n this study, the hydrodynamic filtration technique was empolyed to separate and trap single particles in the comb-like microchannels. The channel structure was designed according to the equivalent flow resistance network system. Micro sensing electrodes were added to the system to measure the impedance in the trapping region of the branch channel for detection of the captured single particles. In the experiments, the channel structure for separation and trapping of single particles was made of Polydimethylsiloxane (PDMS) channel using the lithography and soft lithography techniques, and the electrode chip for impedance measurements was fabricated using the lithography and lift-off techniques. Single microbeads of 20 μm in diameter were successfully trapped at flow rates of 10 μL/min and channel depths of 30 and 50 μm. The measurement results also show that the impedance increased 10% at the frequency range of 20-90 kHz when the particle was captured between the sensing electrodes. This device has great potential for applications in single cells capturing, culturing and monitoring systems.
URI: http://hdl.handle.net/11455/2814
其他識別: U0005-0808201312103400
Appears in Collections:機械工程學系所

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