Please use this identifier to cite or link to this item:
Identification of native Dendrobium species by Randomly Amplified Polymorphic DNA Markers.
|關鍵字:||Dendrobium;石斛蘭;Randomly Amplified Polymorphic DNA;identification;逢機增殖多型性DNA;鑑別||出版社:||園藝學系||摘要:||
本論文係以原生於台灣地區，包括屏東里龍山之長爪石斛(Dendrobium chameleon)等14種，菲律賓地區原生之鴿石斛(D. crumenatum)等2種及大陸地區原生之鐵皮石斛(D. candidum)等3種，共計19種之原生石斛蘭為試驗材料，並以產自台東大武之白花蝴蝶蘭(Phalaenopsis aphrodite)組培苗為遠端對照，進行植物外觀形態及生育特性調查，並以分子層次之逢機增殖多形性DNA片段(RAPD)標誌鑑別原生石斛蘭種原，以瞭解種間親緣關係之遠近及不同地域之生長環境對同種石斛蘭之遺傳變異情形。
Genetic diversity and relatedness were assessed among 19 Dendrobium species such as D. chameleon, D. erumenatum, D. candidum, etc., using 10 randomly amplified polymorphic DNA (RAPD) primers. Of these Dendrobium studied, 14 are native in Taiwan, 2 from the Philippinges and 3 from mainland China. The phalaenopsis aphrodite seedlings derived from tissue culture were included for comparison. The plant morphology and biological traits were also investigated to study ecological effects on genetic composition. The plant growth was influenced by its epiphytic site and intercepted light intensity. Grown on rocks, the plant has smaller pseudobulb and less leaves than that grown in woods. The latter may be two times of the size of the former. Different Dendrobium species could be distinguished by the color,size and shape of flower. Other morphological traits could also be used to separate different species, such as angle of leaf growth, color of pseudobulb, internode length and growth habit. The brilliant orange color of D. clavatum is the most beautiful, follower by pink series of D. falconeri, D. linawianum and D. miyakei. The bright white color of D. crumenatum. and yellow series of D. tosaense are also valuable. The flowering period of individual flower was longest in D. tosaense (15 days) , followed by D. linawinanum (11 d) and D. leptocladum and D. moniliforme (10 d), The shortest flowering period was observed in D. crumenatum and Flickingeria comata with life span less than one day. Self-pollination was found successful in D. clavatum , D. tosaense and F. comate. About 50% of fruit set from self- pollination was obtained in D. equitans, D. leptocladum, D. linawianum and Epigeneium nakaharaei. Cross pollination enhanced fruit set in D. crumenatum, D. falconeri, D. linawinanum and D. moniliforme. Twenty Operon primers (OPE-01~20) were used to screen for polymorphism. The results showed interspecific divergency. The Dendrobium species surveyed were classified into six groups. D. crumenatum from both Green Island and the Philippines as a group, D. miyakei(from both the Philippines and Orchid Island), D. chameleon and Epigeneium sanseiense in group Ⅱ, D. moniliforme, D. candidum, D. tosaense, and D. falconeri (from Ku-kuan, Sun-Link —Sea & Yun-Nan) in group Ⅲ, D. clavatum and D. linawianum in group Ⅳ, D. somai and D. leptocladum in groupⅤ, Flickingeria comata, F. tairukountia and F. finbriata in group Ⅵ. Low similarity value detected from D. miyakei suggested it as another genus. Species in group Ⅱ and Ⅴ may also be treated as independent genera. Genetic diversity as well as morphological difference existed between accessions of the same species from different regions. As in D. falconeri, accessions from Ku-kuan and Sun-Link —Sea are genetically more similar in between than to that from Yun-Nan, China. Geographical distance might affect intra specific similarity.
|Appears in Collections:||園藝學系|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.