Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/28187
標題: 楊桃莖頂組織初代培養之研究
Studies on initial shoot tip culture of carambola (Averrhoa carambola L.) in vitro
作者: 林慈玉
Lin, Tsz-Yu
關鍵字: 楊桃;莖頂培養
出版社: 園藝學系
摘要: 
本研究利用組織培養技術,採用B8、二林、秤錘、台農一號及酸味種楊桃之莖頂加以培養,觀察各品種生長點之型態並探討各栽培品種間最適當的培養方法,期以建立大量迅速之繁殖方法。
由不同品種間莖頂觀察之結果可知,二林及秤錘種之生長點較大,台農一號及酸味種次之,B8楊桃較小。絨毛量方面則以二林及秤錘楊桃較多,台農一號及酸味種次之,B8最少。絨毛顏色均為黃綠色,品種間無明顯不同。
不同品種初代培養之結果發現,以濾紙紙橋培養下能得到較高的成活率,且芽體生長較佳。適當的鹽類濃度依品種而有不同之需求,B8在1/2MS下適合莖頂生長,二林及秤錘為1/8MS,台農一號及酸味種則為1/4MS。
使用不同種類及濃度之生長調節劑以促進莖頂培植體之發育,適當的cytokinin及auxin濃度依品種而有不同之需求。Cytokinin方面,BA以0.1mg/l為適當的濃度,kinetin、zeatin及2ip的影響較小。NAA及IBA適當濃度則分別介於0.01~0.02mg/l及0.02~0.05mg/l之間。
在cytokinin與auxin相互效應的試驗中,B8在1/2MS培養基以IBA 0.02 mg/l配合BA 0.1mg/l效果最佳,二林、臺農一號及酸味種則分別以BA 0.5、0.1及4mg/l添加NAA 0.05mg/l培養於其最適鹽類培養基生長最好。
一般而言,楊桃之品種都可培養於1/4 MS液態紙橋培養基,並添加蔗糖30g/l,cytokinin方面可單獨添加BA 0.1mg/l;auxin方面則品種間差異較大,B8以IBA較為適合,其他則可利用NAA,來達到促進生長之效果。

Summary
This study is conducted to investigate the morphology of the shoot tip of `B8',`Erh-Lin´,` Chun-Choi´,`Tai-nung No.1´ and `Sour´ carambola (Averrhoa carambola L.). The optimum culture methods in vitro for each culitivar were examined in order to establish mass propagation scheme for them.
The observation showed that the apical meristems of `Erh-lin´ and `Chun-Choi´ were bigger, followed by `Tai-nung No.1´ and `Sour´ carambola, and `B8´ was smaller. Quantity of trichome of`Erh-lin' and `Chun-Choi´ were the most, followed by `Tai-Nung No.1´ and `Sour´ carambola, and `B8´ was smaller. The trichome were yellow-green and not any particular difference in the color among culitivars.
All of the culitivars cultured in medium with filter paper bridge resulted in better survival percentage with better bud growth. Optimum salt concentration was different for each cultivar, the optimal salt concentration for shoot tip growth were 1/2MS for `B8´, 1/8MS for `Erh-Lin´ and `Chun-Choi´, 1/4MS for `Tai-Nung No.1´ and `Sour´ carambola.
Various plant growth regulators were used at different concentration to promote the development of the shoot tip, optimum cytokinin and auxin concentration was different for each cultivar. Optimum cytokinin concentration were 0.1mg/l for BA. Kinetin, zeatin and 2ip was the least effective. Optimum NAA and IBA concentration were obtained between 0.01~0.02mg/l and 0.02~0.05mg/l, respectively.
In interactive cytokinin and auxin experiment, explants of `B8´ in 1/2MS medium supplemented with IBA 0.02 mg/l and BA 0.1mg/l grew better. `Erh-Lin´, ` Tai-Nung No.1´ and `Sour´ carambola added BA 0.5、0.1and 4mg/l respectively to each optimum salt concentration contains 0.05 mg/l NAA medium grew better.
The generally growth of camambola was achieved when shoot tip cultured on filter paper bridge medium with 1/4 strength of MS contaning sucrose 30g/l and BA 0.1mg/l of cytokinin. Auxin concentration were IBA for `B8´ and NAA for other culitivars.
URI: http://hdl.handle.net/11455/28187
Appears in Collections:園藝學系

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