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Studies on Shoot Tip Culture of Ceylon Olive (Elaeocarpus serratus L.) in Vitro
|關鍵字:||Ceylon olive;錫蘭橄欖;Shoot tip culture;莖頂組織||出版社:||園藝學系||摘要:||
本研究採用錫蘭橄欖(Elaeocarpus Serratus L.)為試驗材料，探討培養基組成及培養方法對莖頂培養成活之影響，以建立可行之快速繁殖方法。
培植體經初代培養後移至增殖培養基中培養，以1/2MS添加1mg/l BA之培養基培養30日後可獲得增殖之幼梢，再將所獲得之幼梢移至1/2MS添加0.5mg/l zeatin之培養基進行培養，以促進幼梢伸長。
經抽梢培養所得2.5mm之幼梢扦插於1/2MS添加1mg/l NAA及162mg/l PG之固態培養基中暗處理培養7日後，移出瓶外扦插於珍珠石及蛭石(1：1)之Agripot容器中，53日後可獲得發根小苗。
A study of in vitro shoot tip culture of Ceylon olive (Elaeocarpus Serratus L.) was carried out to develop an optimal micropropagation system.
Shoot tips about 5cm in length from vigorous field grown trees were harvested and shoot apex culture was achieved by employing a paper bridge liquid medium containing 1/2MS, 2mg/l kinetin and 30g/l sucrose. Explants were inbubated in darkness for 1 day, followed by in lightness for 29 days. Survived apices were transferred to a solid agar medium containg the same components for further growth.
Multiple shoots were induced by culturing explants in a 1/2MS medium containing 1mg/l BA, 30g/l sucrose and 8g/l agar for 30days. For elongation of these young shoots, 1/2MS medium supplemented with 0.5mg/l zeatin, 30g/l sucrose and 8g/l agar gave a good result.
For rooting,the shoots, above 25mm in height, were cultured in darkness in 1/2MS medium containing 1mg/l NAA, 162mg/l PG, 20g/l sucrose and 7g/l agar for 7 days, followed by in lightness in an Agripot containing a mixture medium of perlite and vermiculite for 53 days.
|Appears in Collections:||園藝學系|
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