Please use this identifier to cite or link to this item:
標題: 利用形態性狀與RAPD標誌在番椒品種鑑別應用之研究
Application of Morphological Traits and Random Amplified Polymorphic DNA (RAPD) Markers on Cultivar Identification of Capsicum annuum L.
作者: 林政潔
Lin, Cheng Chieh
關鍵字: 形態性狀;Morphological;逢機增殖多型性DNA標誌;番椒;品種;鑑別;Traits;Random Amplified Polymorphic DNA (RAPD) Markers;Cultivar;Identification;Capsicum annuum
出版社: 園藝學系
以國內種苗公司的49個番椒品種(Capsicum annuum L.)的外觀形態性狀進
行群叢分析(cluster analysis),並篩選對農友種苗公司的7個品種具有
多型性的RAPD(random amplified polymorphic DNA)標誌之引子(
試品種完全鑑別區分。自110組逢機引子(random primers)中篩選出

Morphological traits and randomly amplified polymorphic DNA
(RAPD) markers are used to identify their relationship of 49
pepper(Capsicum annuum L.) cultivars from Know You and Evergrow
Seed Companies. Twenty five horticultural traits based on the
classification system of National Plant Genetic Resource Center
showed that the morphological traits of seed, leaf, flower, and
fruit can be used to distinguish 49 Capsicum annuum L.
cultivars. By utilization of the differences of 14 fruit
characteristics to test these 49 cultivars that they could not
be fully distinguished by the morphological traits along.A total
of 10 DNA primers are selected from 110 random primers which has
shown the polymorphic patterns among 7 pepper cultivars from
Know You Seed Company. Ten of the 110 primers, i. e. UBC-327,
UBC-484, UBC-002, UBC-005, UBC-019, UBC-020, UBC-042, UBC-043,
UBC-066, UBC-084, showed polymorphism and could distinguish all
49 Capsicum annuum L. cultivars. The least similarity of cluster
analysis is about 81% among cultivars by 10 primers.Pepper
cultivars with UBC-019m(1330bp) or UBC-020j(1800bp) band belong
to group of hot pepper cultivars with UBC-043e(1300bp) band had
strong pungency, narrower fruit shape, and smooth fruit surface.
Hot pepper only showed cultivars with UBC-020j(1800bp) band but
without UBC-043e(1300bp) band are slight pungency , wider fruit
shape, and groove fruit surface, such as 'Peace Star' and '
Group Stars' cultivars of Know You Seed Company. The
dendrograms displayed sweet pepper and hot pepper can be
distinguished through the cluster analysis of using RAPD
markers. Cultivar 'Uranus' showed proximate genetic
relationship with 'Big Star' of Know You Seed Company. And
Know You cultivars of 'Peace Star' and 'Group Stars' were
clustered in the same group with Evergrow Seed Company cultivars
of 'Lady Square' and 'Summer Bell'. The same as 'Vega' and
'Beauty Bell' cultivars of Know You Seed Company and 'Square
Lamuyo', 'Rich Square', and 'Blocky Red' cultivars of
Evergrow Seed Company. The result of genetic relationship among
49 Capsicum annuum L. cultivars based on RAPD markers are
similarity with cluster analysis of morphological traits.
Appears in Collections:園藝學系

Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.