Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/2841
標題: 奈米半球形陣列結構應用於DNA檢測之電化學特性
Electrochemical properties of nano-hemisphere array and its application on DNA nanobiosensor
作者: 林永祥
Lin, Yung-Hsiang
關鍵字: 3D奈米結構感測器;3D Nanostructure, Biochip;SNPs;DNA;EIS;SNPs;DNA;EIS
出版社: 機械工程學系所
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摘要: 
本研究成功利用奈米結構之特性,將其製作成生醫感測晶片應用於單一核苷酸變異(Single nucleotide polyphisms :SNPs)檢測上,並利用電化學阻抗分析(electrochemical impedance spectroscopy,EIS)方式進行檢測來達到省時、快速及高靈敏性。首先利用陽極氧化製程製作陽極氧化鋁模板(AAO),接著在表面濺鍍上一層金,並利電化學沉積金顆粒法於奈米結構上均勻沉積大小約10nm之奈米金顆粒,藉此提升檢測面積,增加感測器靈敏度。由於使用的檢測試片結構有所不同,其3D奈米結構之特性使傳統的Randles等效電路無法求出接附DNA後正確的阻抗值。因此將檢測之實驗數據(頻率、實部、虛部)帶入Zsimp Win電化學數值分析軟體,藉由修正其等效電路擬合出符合之等效電路。
研究發現當加入常相位角元件Q(constant phase element,CPE)並奈米結構之阻值Rn時,其擬合出之卡方值 可達4.5E-4,相較於傳統Randles等效電路卡方值 5.9E-2大幅提升準確性。因此利用修正過後之等效電路可明顯區分出match與mismatch之差異性。當Probe-DNA(T)接附References-DNA(A)為match時,ΔR/R0為30%,而Probe-DNA(T)接附References-DNA(T)為mismatch時,ΔR/R0為62%。利用阻值差異百分比可用來區分病人檢體上的變異性,進而達到未來應用於醫療快篩檢測上。

In the study, the biochip for examining single nucleotide polyphisms (SNPs) which uses the feature of nanostructure has been developed. Using electrochemical impedance spectroscopy (EIS) during the experiment can offer several advantages such as time requirement, good efficiency and high sensitivity. First of all, Anodic Aluminum Oxide (AAO) is fabricated by anodic oxidation. A thin film of gold is sputtered on the reaction area of the biochip and gold nanoparticles in diameter 10nm are deposited on it as well. The preparation is for increasing the area of its surface on detection, so it can improve higher sensitivity. Because of different structure of the reaction area, the traditional Randles equivalent circuit could not analyze the accurate Impedance of DNA. Zsimp Win software is used to modify an appropriate equivalent circuit to match the analyzed numbers with the experimental ones.
Constant phase element (CPE) is also used in the study, and the Chi-square number of new equivalent circuit (X2) is around 4.5x10-4 better than Randles equivalent circuit’s (5.9x10-2). After modifying the equivalent circuit, the difference between match and mismatch can be identified obviously. The results show that its impedance increased 30% when probe-DNA (T) matches reference-DNA (A). On the contrary, it increased up to 62% when probe-DNA (T) mismatches reference-DNA (T). According to the achievement of the research, the percentage of increased impedance (ΔR/R0) can be used to distinguish single nucleotide polyphisms from patients, and it also can be applied in the future on the rapid detections of different diseases.
URI: http://hdl.handle.net/11455/2841
其他識別: U0005-2507201213181100
Appears in Collections:機械工程學系所

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