Please use this identifier to cite or link to this item:
標題: 長實金柑(Fortunella margarita Swingle)莖頂組織培養之研究
Studies on Shoot Tip Culture of Oval Kumquat (Fortunella margarita Swingle) in Vitro
作者: 陳翠英
Tran, Thi-Thuy-Anh
關鍵字: oval kumquat;長實金柑;micropropagation;莖頂培養
出版社: 園藝學系所
引用: References 1. Adam, B. W., R. T. Marks, and A. G. Edwards. 2002. Control of hyperhydricity in Eucalyptus axillary shoot cultures grown in liquid medium. Plant Cell Tiss. Org. Cult. 71: 245-252. 2. Al - Bahrany. A. M. 2002. Effect of phytohormones on in vitro shoot multiplication and rooting of lime Citrus aurantifolia (Christm.) Swing. Scientia Hort. 95: 285-295. 3. Al - Khayri, J. M. and A. M. Al - Bahranny. 2001. In vitro micropropagation of Citrus aurantifolia (lime). Current Science. 81 (9): 1242-1246. 4. Al-Sslem, M. M. and N. S. Karam. 2001. Auxin, wouding, and propagation medium affect rooting response of stem cutting of Arbutus andrachne. HortScience 36(5): 976-978. 5. Amin, M. N. and S. Akhtar. 1993. Regeneration of plants in vitro from seedlings explants of pummelo (Citrus grandis L. Osb.). Plant Tiss. Cult. 3: 71-79. 6. Amin, M. N. and V. S. Jaiswal. 1987. Rapid clonal propagation of guava through in vitro shoot proliferation in nodal explants of mature trees. Plant Cell Tiss. Org. Cult. 9: 235-243. 7. Amin, M. N. and V. S. Jaiswal. 1993. In vitro response of apical bud explants from mature trees of jackfruit (Artocarpus heterophyllus). Plant Cell Tiss. Org. Cult. 33 (1): 59-65. 8. Barbara, N., K. Miczynski, and L. Hudy. 2004. Sugar uptake and utilisation during adventitious bud differentiation on in vitro leaf explants of ‘Wegierka Zwykla' plum (Prunus domestica). Plant Cell Tiss. Org. Cult. 76: 255-260. 9. Barcelo-Munoz, A., C. L. Encina, E. Simon-Perez, and F. Pliego-Alfaro.1999. Micropropagation of adult avocado. Plant Cell Tiss. Org. Cult. 58: 11-17. 10. Barciszewski, J., G. S. Siboska, I. S. Ranttan, and B. F. C. Clark. 2000. Occurrence, biosynthesis and properties of kinetin (N6- Furfuryladenine). Plant Growth Regul. 32: 257-265. 11. Barlass, M. and K. G. M. Skene. 1982. In vitro plantlet formation from Citrus species and hybrids. Scientia Hort. 17: 333-341. 12. Baydar, N. G., S. Ulger, and S. Cetin. 2006. Effect of endogenous phenolic compounds and hormones on in vitro shoot tip culture of grapevine. J. Hort. Sci. Bio. 81 (3): 429-434. 13. Begum, F., M. N. Amin, S. Islam, M. A. K. Azad, and M. M. Rehman. 2003. In vitro regeneration from Cotyledon-derived callus of three varieties pummelo (Citrus grandis [L.] Osb.). J. Bio. Sci. 3 (8): 751-759. 14. Beloualy, N. 1991. Plant regeneration from callus of three Citrus rootstocks. Plant Cell Tiss. Org. Cult. 24: 29-34. 15. Berthon, J. Y., S. Ben Tahar, T. Gaspar, and N. Boyer. 1990. Rooting phases of shoots of Sequoiadendron gigantum in vitro and their requirements. Plant Physiol. Biochem. 28: 631-638. 16. Bhat, S. R., P. Chitralekha, and K. P. S. Chandel. 1992. Regeneration of plants from long term root culture of lime, Citrus aurantifolia (Christm) Swing. Plant Cell Tiss. Org. Cult. 29: 19-25. 17. Bordon, Y., J. L. Guardiola, and A. Garcia-Luis. 2000. Genotype affects the morphogenic response in vitro of epicotyl segments of Citrus rootstocks. Ann. Bot. 86: 159-166. 18. Buban, T. 2000. The use of benzyladenine in orchard fruit growing. Plant Growth Regul. 32: 381-390. 19. Burger, D.W. and W. P. Hackett. 1986. Gradients of adventitious bud formation on excised epicotyl and root sections of citrus. Plant Sci. 43: 229-232. 20. Carimi, F., M. C. Tortorici, F. D. Pasquale, and F. G. Crescimanno. 1998. Somatic embryogenesis and plant regeneration from undeveloped ovules and stigma/style explants of sweet orange navel group (Citrus sinensis (L.) Osb.). Plant Cell Tiss. Org. Cult. 54: 183-189. 21. Carvalho, L. C., M. L. Osorio, M. M. Chaves, and S. Amancio. 2001. Chlorophyll fluorescence as an indicator of photosynthetic functioning of in vitro grapevine and chestnut plantlets under ex vitro acclimatization. Plant Cell. Tiss. Org. Cult. 67: 271-280. 22. Castillo, B., M. A. L. Smith, D. L. Madhavi, and U. L. Yadava. 1997. Interactions of irradiance level and iron chelate source during shoot tip culture of Carica papaya L. HortScience 32(6): 1120-1123. 23. Chatuverdi, H. C. and G. Mitra. 1974. Clonal propagation of citrus from somatic callus culture. HortScience 9: 118-120. 24. Chiari, A. and M. P. Bridgen. 2002. Meristem culture and virus eradication in Alstroemeria. Plant Cell Tiss. Org. Cult. 68: 49-55. 25. Choi, Y., D. Yang, and K. Choi. 1998. Induction of somatic embryo by macrosalt zygotic embryos of Panax ginge. Plant Cell Tiss. Org. Cult. 52: 177-181. 26. Costa, M. G. C., V. S. Alves, and E. R. G. Lani. 2004. Morphogenic gradients of adventitious bud and shoot regeneration in epicotyl explants of Citrus. Scientia Hort. 100: 63-74. 27. Dale, P. J. and V. Cheyne. 1993. The elimination of clover diseases by shoot tip culture. Ann. Appl. Biol. 123(1): 25-32. 28. Damiano, C., C. Antonella, C. Emilia, Q. Roverta, and B. Giovanna. 1991. Some factors effecting the induction and the expression of rooting in different fruit species in vitro. Acta Hort. 300: 211-224. 29. Das, D. K., P. N. Shiva, and N. Bhalla-Sarin. 1999. Multiple shoot induction and plant regeneration in lichi (Lichi chinensis Sonn.). Plant Cell Rep. 18(7/8): 691-695. 30. De Klerk, G. J., M. Keppel, J. Brugge, and H. Meekes. 1995. Timing of the phases in adventitious root formation in apple microcuttings. J. Exp. Bot. 46: 965-972. 31. Debergh, P. C. and L. J. Maene. 1981. A scheme for commercial propagation of ornamental plant by tissue culture. Scientia Hort. 14: 335-345. 32. Dimassi. K., V. Chouliaras, G. Diamantidis, and I. Therios. 2003. Effect of iron and auxins on peroxidase activity and rooting performance of three citrus rootstocks in vitro. J. Plant. Nutr. 26 (5):1023-1034. 33. Dodds, J. H. 1982. Experiment in plant tissue culture. Cambridge University Press. pp. 45-49. 34. Duran-Vila, N., V. Ortega, and L. Navarro. 1989. Morphogenesis and tissue cultures of three Citrus species. Plant Cell Tiss. Org. Cult. 16: 123-133. 35. Duran-Vila, N., Y. Gogorcena, V. Ortega, J. Ortiz, and L. Navarro. 1992. Morphogenesis and tissue culture of sweet orange (Citrus sinensis (L.) Osb.): Effect of temperature and photosynthetic radiation. Plant Cell Tiss. Org. Cult. 29: 11-18. 36. Edriss, M. H. and D.W. Burger. 1984. In vitro propagation of Troyer citrange from epicotyl segments. Scientia Hort. 23: 159-162. 37. Feng, G., G. Yifu, and Z. Pinbo. 2000. Production and deployment of virus-free sweetpotato in China. Crop Prot. 19: 105 - 111. 38. Fotopoulos, S. and T. E. Sotiropoulos. 2005. In vitro rooting of PR 204/84 rootstock (Prunus persica x P. amygdalus) as influenced by mineral concentration of the culture medium and exposure to darkness for a period. Afri. J. Biotech. 3 (11): 576-584. 39. Fukui H., M. Sugiyama, and M. Nakamura. 1989. Shoot tip culture of Japanese persimmon (Diospyros Kali Thunb.). J. Japan. Soc. Hort. Sci. 58(1): 43-47. 40. Garcia - Luis, A., R.V. Molina, V. Varona, S. Castello, and J. L. Guardiola. 2006. The influence of explant orientation and contact with the medium on the pathway of shoot regeneration in vitro in epicotyl cuttings of Troyer citrange. Plant Cell Tiss. Org. Cult. 85 (2): 137 - 144. 41. Garcia, J. L., J. Troncoso, R. Sarmiento, and A. Troncoso. 2002. Influence of carbon source and concentration on the in vitro development of olive zygotic embryo and explants raised from them. Plant Cell Tiss. Org. Cult. 69: 95-100. 42. Garcia-Luis, A., Y. Bordon, J. M. Moreira-Dias, R.V. Molina, and J. L. Guardiola. 1999. Explant orientation and polarity determine the morphogenic response of epicotyl segments of Troyer citrange. Ann. Bot. 84: 715-723. 43. George, E. F. 1993. Plant propagation by tissue culture. I. The technology. Exegetics Ltd., England. 44. George, E. F. and P. D. Sherrington. 1984. Plant propagation by tissue culture: Handbook and dictionary of commercial laboratories, (ed. By George, E. F. and P. D. Sherrington.) Eastern Press reading, Berk, England: 125-330. 45. Germana, M. A., Y. Y. Wang, M. G. Barbagallo, G. Iannolino, and F. G. Crescimanno. 1994. Recovery of haploid and diploid plantlets from anther culture of Citrus clementina and Citrus reticulata Blanco. J. Hort. Sci. 69: 473-480. 46. Ghorbel, R., L. Navarro, and N. Duran-Vila. 1998. Morphogenesis and regeneration of whole plants of grapefruit (Citrus paradisi), sour orange (C. aurantium) and alemow (C. macrophylla). J. Hort. Sci. Bio. 73: 323-327. 47. Gibson, S. I. 2000. Plant sugar-response pathways. Part of a complex regulatory web. Plant Physiol. 124: 1532-1539. 48. Gill, M. I. S., Z. Singh, B. S. Dhillon, and S. S. Gosal. 1994. Somatic embryogenesis and plantlet regeneration on calluses derived from seedling explants of Kinnow mandarin (Citrus nobilis Lour × Citrus deliciosa Tenora). J. Hort. Sci. 69: 231-236. 49. Goh, C. J., G. E. Sim., C. L. Morales, and C. S., Loh. 1995. Plantlet regeneration through different morphological pathways in pummelo tissue culture. Plant Cell Tiss. Org. Cult. 43: 301-303. 50. Grinblat, V. 1972. Differentiation of citrus stem in vitro. J. Amer. Soc. Hort. Sci. 97: 599-603. 51. Gubbuk, H. and M. Pekmezci. 2006. In vitro propagation of banana (Musa spp.) using thidiazon and activated charcoal. Acta Agriculturae Scandinavia Section B- Soil and Plant Science. 56: 65-69. 52. Hackett, W. P. 1985. Juvenility, maturation and rejuvenility in woody plant. Hort. Rev. 7: 109-147. 53. Hassanein, A. M. and M. M. Azooz. 2003. Propagation of Citrus reticulata via in vitro seed germination and shoot cuttings. Biol. Plant. 47: 173-177. 54. Hausman, J. F. 1993. Changes in peroxidase activity, auxin level and ethylene production during root formation by poplar shoots raised in vitro. Plant Growth Regul. 13: 263-268. 55. Heloir, M. C., J. C. Fournioux, L. Oziol, and R. Bessis. 1997. An improved procedure for the propagation in vitro of grapevine (Vitis vinifera cv. Pinot noir) using axillary-bud microcuttings. Plant Cell Tiss. Org. Cult. 49: 223-225. 56. Hiregoudar, L. V., H. G. Ashok Kumar, and H. N. Murthy. 2005. In vitro culture of Feronia limonia (L.) Swingle from hypocotyl and internodal explants. Biol. Plant. 49: 41-45. 57. Hu, C. Y. and P. J. Wang. 1984. Meristem, shoot tip and bud cultures: In handbook of plant cell culture. 1: 177-277. 58. Huang, T., S. L. Peng, G. F. Dong, L. Y. Zhang, and G. G. Li. 2002. Plant regeneration from leaf-derived callus in Citrus grandis (pummelo): Effect of auxins in callus induction medium. Plant Cell Tiss. Org. Cult. 69: 141-146. 59. Hughes, K. W. 1981. In vitro ecology: Exogenous factors affecting growth and morphogenesis in plant culture systems. Environ. Exp. Bot. 21: 281-288. 60. Ilczuk, A., T. Winkelmann, S. Richartz, M. Witomska1, and M. Serek. 2005. In vitro propagation of Hippeastrum x chmielii Chm. Influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems. Plant Cell Tiss. Org. Cult. 83: 339-346. 61. Isutsa, D. K. 2004. Rapid micropropagation of passion fruit (Passiflora edulis Sims.) varieties. Scientia Hort. 99: 395-400. 62. Jia, Y. J., F. Chen, H. H. Lin, Y. L. Cao, and Y. Y. Luo. 1997. In vitro culture of different explant from Fortunella margarita (Lour.) Swing. J. Sichuan Univ. (nat. Sci. Ed.) 34: 344 - 348. 63. Jones, O. P. 1991. The role of biotechnology in the multiplication and improvement of wood plant. Acta Hort. 289: 35-43. 64. Kadota, M., K. Imizu, and T. Hirano. 2001. Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear. Scientia Hort. 89: 207-215. 65. Kitto, S. L. and M. J. Young. 1981. In vitro propagation of Carrizo citrange. HortScience 16: 305-306. 66. Klerk, G. J. D., J. T. Brugge, and S. Marinova. 1997. Effectiveness of indoleacetic acid, indolbuteric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork9'. Plant Cell. Tiss. Org. Cult. 49: 39-44. 67. Koizumi, M. 1998. Production systems of fruit tree nurseries to control graft-transmissible disease. J. Japan. Soc. Hort. Sci. 67 (6): 1093-1097. 68. Kotsias, D. and P. A. Roussos. 2001. An investigation on the effect different plant growth regulating compounds in vitro shoot tip and node culture of lemon seedlings. Scientia Hort. 89: 115-128. 69. Kozai, T. 1991. Photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium. Plant Cell. Tiss. Org. Cult. 25: 107-115. 70. Kozai, T., C. Kubota, and B. R. Jeong. 1997. Environmental control for the large-scale production of plants through in vitro techniques. Plant Cell. Tiss. Org. Cult. 51: 49-56. 71. Leblay, C., E. Chevraeau, and L.M. Raboin. 1991. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.). Plant Cell Tiss. Org. Cult. 25: 99 - 105. 72. Lee, M.Y. and Y. S. Yang. 1994. Studies on shoot tip culture of guava (Psidium guajava L.) in vitro. Horticulture NCHU. 19: 47 - 59. 73. Leontive-Orlov, O. A., L. A. Leontiva-Orlova, A. Mossi, and R. L. Cansian. 2005. Study of the influence of major ions and benzylaminopurin on proliferation in vitro of microshoots of apple, plum, trifoliata orange and black currant by a systematic variance method. Acta Hort. 632: 249-253. 74. Liao, M. F. and Y. S. Yang. 2005. Effect of growth regulators on shoot tip culture of ‘G755' avocado rootstock. Seed and Nursery (Taiwan). 7(4): 33-40. 75. Lin, T. Y. and Y. S. Yang. 2002. Studies on initial shoot tip culture of carambola (Averrhoa carambola L.) in vitro. Horticulture NCHU. 27(3): 13-24. 76. Ling, J. T. and M. Iwamasa. 1997. Plant regeneration from embryogenic calli of six Citrus related genera. Plant Cell Tiss. Org. Cult. 49: 145-148. 77. Lo, H. P. and Y. S Yang. 2000. Studies on shoot tip culture of ceylon olive (Elaeocarpus serratus L.)in vitro. Horticulture NCHU. 25(3):13- 25. 78. Lo, H. P. and Y. S Yang. 2006. The effect of auxin treatment on shoot proliferation and rooting of Ceylon olive in vitro. Seed and Nursery (Taiwan). 8(1): 59-67. 79. Lux-Endrich, A., D. Treutter, and W. Feucht. 2000. Influence of nutrients and carbohydrate supply on the phenol composition of apple shoot culture. Plant Cell Tiss. Org. Cult. 60: 15-21. 80. Maggon, R. and B. D. Singh. 1995. Promotion of adventitious bud regeneration by ABA in combination with BAP in epicotyl and hypocotyl explants of sweet orange (Citrus sinensis L. Osbeck). Scientia Hort. 63: 123-128. 81. Min, T. H. and Y. S Yang. 1998. Studies on shoot tip culture of atemoya and cherimoya in vitro. Horticulture NCHU. 23(2): 47-61. 82. Monteuuis, O. and M. C. Bon. 2000. Influence of auxins and darkness on in vitro rooting of micropropagated shoots from mature and juvenile Acacia mangium. Plant Cell Tiss. Org. Cult. 63: 173-177. 83. Moore, G. A. 1986. In vitro propagation of Citrus rootstocks. HortScience 21: 300-301. 84. Moreira - Dias, J. M., R.V. Molina, J. L Guardiola, and A. Garcia-Luis. 2001. Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange. Scientia Hort. 87: 275-290. 85. Moreira-Dias, J. M., R.V. Molina, Y. Bordon, J. L. Guardiola, and A. Garcia-Luis. 2000. Direct and indirect shoot organogenic pathways in epicotyl cuttings of Troyer citrange differ in hormone requirements and in their response to light. Ann. Bot. 85: 103-110. 86. Morini, S., M. Trinci, and M. Zacchini. 1991. Effect of different photoperiods on in vitro growth of Mr.S. 2/5 plum rootstock. Plant Cell Tiss. Org. Cult. 25: 141-145. 87. Morini, S., R. Sciutti, and P. Fortuna. 1992. In vitro growth response of Prunus cerasifera shoots as influenced by different light-dark cycle and sucrose concentrations. Plant Cell Tiss. Org. Cult. 28: 245-248. 88. Morini, S., R. Sciutti, R. Muleo, and P. Fortuna. 1991. Growth patterns of in vitro cultured shoot tips as influenced by different light-dark regimes. Acta Hort. 289: 137-138. 89. Mrini, S. and S. Perrone. 2006. Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks. Biol. Plant. 50 (3): 429-432. 90. Murashige, T. 1974. Plant propagation through tissue cultures. Ann. Rev. Plan Physiol. 25: 135-166. 91. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 13: 473-497. 92. Nomura, K., S. Matsumoto, K. Masuda, and M. Inoue. 1998. Reduced glutathione promotes callus growth and shoot development in a shoot tip culture of apple root stock M26. Plant Cell Rep. 17: 597- 600. 93. Ochatt, S. J. and H. C. Osvaldo. 1982. In vitro meristem culture of M.4 apple (Malus pumila Mill.). I. Optimal nutrient medium. 2(1): 39-48. 94. Paudyal, K. P. and N. Haq. 2000. In vitro propagation of pummelo (Citrus grandis L.Osbeck). In Vitro Cell. Dev. Biol. Plant. 36: 511-516. 95. Perez, R. M., A. M. Galiana, L. Navarro, and N. Duran-Vila. 1998. Embryogenesis in vitro of several Citrus species and cultivars. J. Hort. Sci. Bio. 73: 796-802. 96. Perez-Molphe-Balch, E. and N. Ochoa-Alejo. 1997. In vitro plant regeneration of Mexican lime and mandarin by direct organogenesis. HortScience 32: 931-934. 97. Perez-Tornero, O. and L. Burgos. 2000. Different media requirement for micrppropagation of apricot cultivars. Plant Cell Tiss. Org. Cult. 63: 133-141. 98. Pevalek, K. B., C. Michler, and S. Jelaska. 1994. Microclonal multiplication of wild cherry (Prunus savium L.) from shoot tips and root sucker bud. Acta Bot. 53: 31-38. 99. Phan, C. T. 1991. Vitreous state in vitro culture: ethylene versus cytokinin. Plant Cell Rep. 9: 517-519. 100. Ponchia, G. and M. Gardiman. 1992. Research on in vitro propagation of mulberry (Murus Alba L.). Acta Hort. 314: 99-104. 101. Ritu, M., R. K., R. Mahajian, and R. Kaur. 2000. Effect of size of shoot tip apical meristem on its survival and virus elimination in two strawberry cultivars Chandler and Fern. Research on Crops 1 (3): 379-381. 102. Rival, A., F. Bernard, and Y.Mathieu. 1997. Changes in peroxidase activity during in vitro rooting of oil palm (Elaeis guineensis Jacq.). Scientia Hort.71: 103-l 12. 103. Rodriguez, R., C. Diaz-Sala, L. Cuozzo, and G. Ancora. 1991. Pear in vitro propagation using a double-phase culture system. HortScience 26: 62-64. 104. Ruzic, D., M. Saric, R. Cerovicc, and L. Culafic. 2000. Relationship between the concentration of macroelements, their uptake and multiplication of cherry rootstock Gisela 5 in vitro. Plant Cell Tiss. Org. Cult. 63: 9-14. 105. Sauton, A., A. Mouras, and A. Lutz. 1982. Plant regeneration from citrus root meristems. J. Hort. Sci. 57: 227-231. 106. Shackel, K. A., V. Novello, and E. G. Sutter. 1990. Stomatal function and cuticular conductance in whole tissue-cultured apple shoots. J. Amer. Soc. Hort. Sci. 115 (3): 468-472. 107. Shawkatali and Bushramirza. 2006. Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explant type and hormone concentration. Acta Bot. Croat. 65 (2), 137-146. 108. Shim, S. W., E. J. Hahn, and K. Y. Paek. 2003. In vitro and ex vitro growth of grapevine rootstock ‘5BB' as influenced by number of air exchanges and the presence or absence of sucrose in culture media. Plant Cell Tiss. Org. Cult. 75: 57-62. 109. Sim, G. E., C. J. Goh, and C.S. Loh. 1989. Micropropagation of Citrus mitis Blanco multiple bud formation from shoot and root explants in the presence of 6-benzylaminopurine. Plant Sci. 59: 203-210. 110. Singh, S., B. K. Ray, S. Bhattacharyya, and P. C. Deka. 1994. In vitro propagation of Citrus reticulate Blanco and Citrus limon Burm. F. HortScience 29 (3): 214-216. 111. Skirvin, R. M., M. C. Chu, and H. Rukan. 1979. Tissue culture of peach, sweet and sour cherry and apricot shoot tips. Proc. 124 th Ann. Meeting Illi. State Hort. Soc: 30-38. 112. Smith, E. F., A. V. Roberts, and J. Mottley. 1990. The preparation in vitro of Chrysanthemum for transplantation to soil. 2. Improved resistence to desiccation conferred by paclobutrazol. Plant Cell Tiss. Org. Cult. 21: 133-140. 113. Taylor, J. L. S. and J. V. Staden. 2001. The effect of nitrogen and sucrose concentration on the growth of Eucomis autumnalis (Mill.) Chitt. Plantlets in vitro and on subsequent anti-inflammatory activity in extracts prepared from the plantlets. Plant Growth Regul. 34: 49-56. 114. Terwary, P. K. and S. Oka. 1999. Simplified clonal multiplication of mulberry using liquid shake culture. Plant Cell Tiss. Org. Cult. 59(3): 223-226. 115. Thomas, J. C., and F. R. Katterman. 1986. Cytokinin activity induced by thidiazuron. Plant Physiol. 81: 681-683. 116. Thomas, P. and M. B. Ravindra. 1997. Shoot tip culture in mango: influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. J. Hort. Sci. 72 (5): 713 - 722. 117. Usman, M., Sana. M., and B. Fatima. 2005. In vitro multiple shoot induction from nodal explants of Citrus cultivar. J. Cent. Euro. Agri. 6 (4): 435 - 442. 119. Wang, C. J. and Y. S. Yang. 1999. Studies on shoot tip culture of loquat (Eriobotrya japonica Lindl.) in vitro. Horticulture NCHU. 24 (3): 1- 14. 120. Wang, J. 2001. Shoot tip culture and production of virus-free longan plantlet. Acta Hort. 558: 155-160. 121. Wang, Q., M. Mawassi, P. Li, R. Gafny, I. Sela, and E. Tanne. 2003. Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci. 165: 321-327. 122. Williams, R. R. and A. M. Taji. 1991. Effect of temperature, gel concentration and cytokinins on vitrification of Olearia microdisca (J.M. Black) in vitro shoot cultures. Plant Cell Tiss. Org. Cult. 26: 1-6. 123. Yancheva, S. D., S. Golubowicz , E. Fisher , S. Yadun, and M. A. Flaishman. 2003. Auxin type and timing of application determine the activation of the developmental program during in vitro organogenesis in apple. Plant Sci. 165: 299 - 309. 124. Yang, L., C. J. Xu, G. B. Hu, and K. S. Chen. 2006. Direct shoot organogenesis and plant regeneration in Fortunella crassifolia. Biol. Plant. 50 (4): 729-732. 125. Yang, Z. and P. Ludders. 1993. Effect of growth regulator and media on in vitro shoot tip culture of different cultivars of mango (Mangifera indica L.) rootstocks. Acta Hort. 341: 240-247. 126. Yeh, J. Y. and Y. S. Yang. 1999. Studies on shoot tip culture of Indian jujube (Zizyphus mauritiana Lam.) in vitro. Horticulture NCHU. 24 (3): 15- 28. 127. Yolande, P., P. Doumas, L. Lardet, and M. Carron. 1997. Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation. Plant Cell Tiss. Org. Cult. 47: 239-245. 128. Yu, H. J. and Y. S. Yang. 1989. Studies on shoot tip culture of wax apple in vitro. Horticulture NCHU. 14: 31- 43. 129. Yu, T. A., S. W. Weng, and Y. S. Yang. 1993. Studies on shoot tip culture of passionfruit in vitro. Horticulture NCHU. 18: 29 - 44. 130. Zacchini. M., S. Morini, and C. Vitagliano. 1997. Effect of photoperiod on some stomatal characteristics of in vitro cultured fruit tree shoots. Plant Cell Tiss. Org. Cult. 49: 195-200. 131. Zhou, Y. H., D. P. Guo, Z. J. Zhu, and Q. Q. Qian. 2005. Effects of in vitro rooting environments and irradiance on growth and photosynthesis of strawberry plantlets during acclimatization. Plant Cell Tiss. Org. Cult. 81: 105-108. 132. Zimmerman, T.W. and R. Scorza. 1994. Benzyladenine and shortened light/dark cycles improve in vitro shoot proliferation of peach. HortScience. 29: 695-698.
本研究採用長實金柑(Fortunella margarita Swingle)為試驗材料,探討培養基條件及培養基組成分對莖頂培養成活之影響,以求建立可行之繁殖方法。
在植株生長期,分別於不同生長季節,採取長約1cm之莖頂,切取長約0.5mm帶4片葉原體大小之生長點,植於MS添加30g/l蔗糖、8g/l洋菜之固態培養基進行初代培養,接著將經由初代培養後之培殖體,移至含MS添加0.1mg/l BA、0.01mg/l IBA、0.1mg/l kinetin、30g/l蔗糖及8g/l洋菜之固態培養基繼續培養,可促進培植體之生長。
以MS添加1mg/l BA、0.1mg/l IBA、30g/l蔗糖及8g/l洋菜之固態培養基培養30日則可以獲得較佳的增殖率及芽體。
將經由增殖培養所得之10mm長之枝梢,培養於MS添加5mg/l NAA、30g/l蔗糖及8g/l洋菜,進行1週的暗處理,接著將枝梢移至MS添加30g/l蔗糖及8g/l洋菜,不含任何植物生長素之固態培養基培養5週後,即可獲得發根之植株。

Shoot tip culture of oval kumquat (Fortunella margarita Swingle) was studied and the optimal micropropagation conditions for establishment, shoot multiplication and rooting in vitro were investigated.
Shoot tips about 1 cm in length were harvested, shoot apices in 0.5 mm length with 4 leaf primordial were excised under microscope and cultured on MS solid medium containing 30 g/l sucrose, 8 g/l agar for the highest survival percentage in initial culture. Survived explants were transferred to MS solid medium containing 30 g/l sucrose, 8 g/l agar adding 0.1 mg/l BA, 0.01 mg/l IBA and 0.1 mg/l kinetin for further growth.
Multiple shoots were induced by MS solid medium containing 30 g/l sucrose, 8 g/l agar adding 1 mg/l BA and 0.1 mg/l IBA.
For rooting, the shoots grew more than 10 mm in length, were cultured in darkness pretreatment on MS solid medium with 5 mg/l NAA, 30g/l sucrose, and 8 g/l agar for one week and then transplanted to MS solid medium without auxin for 5 weeks
其他識別: U0005-1307200716162200
Appears in Collections:園藝學系

Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.