Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/28966
標題: 黃道報歲蘭之授粉、種子發育與發芽以及報歲蘭之品種特性比較
Hand pollination, seed development and in vitro germination of Cymbidium sinensis 'Huang-dao' and variety characteristics of Cymbidium sinensis (Jackson ex Andr.) Willd.
作者: 徐權君
Shiu, Quan-Jun
關鍵字: 報歲蘭;Cymbidium sinensis (Jackson ex Andr.) Willd.;種子發育與發芽;品種特性;seed development and in vitro germination;variety characteristics
出版社: 園藝學系所
引用: 丁川翊. 2004. 朱槿雜交育種之改進. 國立中興大學園藝系研究所碩士論文 95 pp. 王博仁. 1981. 蕙蘭屬的無菌播種與器官分化. 中央研究院植物研究所專刊第四號(張唯勤編) p. 22-30. 台北. 行政院農業委員會. 2007a. 80-94年植物品種權品種圖鑑(周明燕編). 145 pp. 行政院農業委員會種苗改良場. 台中. 農委會農糧署. 南投. 行政院農業委員會. 2007b. 95年植物品種權年鑑(周明燕編). 91 pp. 行政院農業委員會種苗改良場. 台中. 農委會農糧署. 南投. 朱根發、葉秀斌、陳明莉、王碧青. 2003. 培養基不同成分對墨蘭根莖分化成苗的影響. 中南林學院學報23(5): 42-44. 李志仁. 1991. 報歲蘭與素心蘭之開花與種子無菌播種發芽之研究. 國立台灣大學園藝學研究所碩士論文. 94 pp. 李哖. 1990. 蘭之胚培養. 中國園藝 36(4): 223-244. 李哖. 1997a. 素心蘭、四季蘭和報歲蘭之無菌發芽. 海峽兩岸森林生物技術及環境變遷對森林生態系之影響研討會論文集(姜家華、黃麗春 編) p. 500-509. 國立台灣大學農學院森林學研究所等. 台北. 李哖. 1997b. 國蘭類無菌繁殖技術. 園藝作物組織培養研究與技術手冊(沈再發、陳俊季、文紀鑾 編) p. 81-93. 農林廳種苗改良繁殖場. 臺中. 呂依倫. 1988. 素心蘭與鳳蘭之無菌播種與器官分化. 國立台灣大學園藝學研究所碩士論文. 170 pp. 林讚標. 1988. 拜歲蘭. In台灣蘭科植物,第二卷. p. 126-128. 南天書局. 台北. 周鎮. 1986. 墨蘭. In台灣蘭圖鑑(地生蘭篇). p. 23-43. 創譯出版社. 台中. 邱金春、王博仁. 1985. 嘉德麗雅蘭與蕙蘭種子發芽與芽的形成. 中國園藝 31: 10-22. 陳少宏. 2002. 線藝蘭初探–試論國蘭的遺傳變異與選育之三. 廣東園林 3: 44–47. 陳心啟. 1999. 中國植物志. vol. 18. p. 215–216. 科學出版社. 北京. 張志勝、歐秀娟. 1995. 墨蘭的組織培養. 園藝學報 22(3): 303-304. 張正. 1997. 素心蘭和報歲蘭組織培養與繁殖之研究. 國立台灣大學園藝學研究所博士論文. 121 pp. 張正、宋珮綺、張芝瑄、陳盈君、林永浩. 2006. 臺灣白及種子發育與貯藏之研究. 植物種苗 8(3): 29-38. 張耀乾. 1989. 臺灣一葉蘭之組織培養與報歲蘭根莖之器官分化. 國立台灣大學園藝學研究所碩士論文. 125 pp. 彭曉明、曾宋君、張京麗、趙逢畔. 2000. 三種墨蘭的瓶播培養(簡報). 熱帶亞熱帶植物學報 8(1): 60-62. 彭雙松. 1988. 名品解說. In台灣蘭蕙新輯. p. 156–198. 富蕙圖書出版社. 苗栗. 黃家平、戴思蘭. 1998. 中國蘭花品種數量分類初探. 北京林業大學學報. 20(2): 38–43 黃敏展. 2002. 花色之測色和表色. In亞熱帶花卉學總論. p. 133–138. 國立中興大學園藝系. 台中. 黃瑞啟. 2005. 蕙蘭產銷概況. p. 1-15. 蕙蘭病蟲害防治講習會–中華盆花發展協會. 傅雪琳、張志勝、何平、歐秀娟、何瓊英. 2000. 墨蘭根狀莖綠芽分化的研究. 華南農業大學學報21(3): 53-55 楊冬之、劉海娟、羅毅波、耿盼盼、郭採茹、周波. 2007. 國蘭品種分類研究. 安徽農業科學 35(29): 9242–9243. 陳汝民、葉慶生、王小菁、王曉明、郭周義. 1995. 墨蘭種子胚的發育和培養初步研究. 熱帶亞熱帶植物學報3(4): 72-75. 葉秀璘、郭俊彥. 1995. 墨蘭雌配子體和胚胎發生. 熱帶亞熱帶植物學報 3(1): 54-58. 葉章龍. 2003. 國蘭與蘭文化. 華節師範高等專科學校學報. 21(1): 88-92. 廖敏君. 1992. 素心蘭和報歲蘭根莖誘導芽體和芽生長之研究. 國立台灣大學園藝學研究所碩士論文. 98 pp. 廖曼莉. 1995. 培養基成分、光線及溫度對報歲蘭根莖生長與分化的影響. 國立台灣大學園藝學研究所碩士論文. 84 pp. 廖曼莉、張莉欣、李哖. 1997. 素心蘭與報歲蘭根莖之器官分化與大量繁殖. 園藝種苗科技研發成果發表會專集(沈再發、陳駿季、廖文偉 編). p 593-629. 台灣省政府農林廳種苗改良場. 劉方農、劉連仁. 2003. 蘭花的形態、生活和繁殖. 西昌農業高等專科學校學報 17(1): 23-27. 魯雪華、郭文杰、林勇. 1999. 墨蘭無菌播種和植株再生. 亞熱帶植物通訊28(1): 34-37. 蕭宇倫. 2000. 培養基成分對報歲蘭 ‘十八學士’ 暨其雜交種根莖增殖生長與芽體誘導分化之影響. 國立台灣大學園藝學研究所碩士論文. 112 pp. Arditti, J. 1992. Reproduction. In Fundamentals of orchid biology. p. 533-569. New York, USA: John Wiley & Sons Inc. Arditti, J., and R. L. Knauft. 1969. The effect of auxin, actinomycin D, ethionine, and puromycin on post-pollination behavior of Cymbidium (Orchidaceae) flower. Amer. J. Bot. 56: 394-397. Arditti, J., D. C. Jeffrey, and B. H. Flick. 1971. Post-pollination phenomena in orchid flowers. III. Effects and interactions of auxin, kinetin or gibberellin. New Phytol. 70(6): 1125-1141. Brewbaker, J. L., and B. H. Kwack. 1963. The essential role of calcium ion in pollen germination and pollen tube growth. Amer. J. Bot. 50: 859-865. Calzoni, G. L., A. Speranza, and N. Bagni. 1979. In vitro germination of apple pollens. Sci. Hort. 10: 49-55. Chang, C., and W. C. Chang. 2000. Effect of thidiazuron on bud development of Cymbidium sinensis Willd. in vitro. Plant Growth Regul. 30: 171-175. Chang, C., Y. C. Chen, and H. F. Yen. 2005. Protocorm or rhizome? The morphology of seed germination in Cymbidium dayanum Reichb. Bot. Bull. Acad. Sin. 46: 71-74. Chen, S. C., and T. Tang. 1982. A general review of orchid flora of China. In J. Arditti (ed.), Orchid biology: Reviews and perspectives, II. Cornell University Press, Ithaca. p. 39-81. Chen, Y. Q., X. Liu, and Y. Liu. 2005. In vitro plant regeneration from the immature seeds of Cymbidium faberi. Plant Cell Tissue Organ Cult. 81: 247-251 Chung M. Y., and M. G. Chung. 2003. The breeding systems of Cremastra appendiculata and Cymbidium goeringii: high levels of annual fruit failure in two self-compatible orchids. Ann. Bot. Fenn. 40: 81-85. Deb, C. R., and Temjensangba. 2006. In vitro propagation of terrestrial orchid, Malaxis khasiana Soland ex. Swartz through immature seed culture. Indian J. Exp. Biol. 44: 762-766. Du Puy, D., and P. Cribb. 1988. The classification of Cymbidium. In The genus Cymbidium. Timber Press, Oregon. pp. 50-194. Huang, B. Q., X. L. Ye, E. C. Yeung, and S. Y. Zee. 1998. Embryology of Cymbidium sinensis: the microtubule organization of early embryo. Ann. Bot. 81: 741-750. Huang, C. L., and H. Okubo. 2005a. Effect of carbon sources in in vitro morphogenesis from rhizomes of Cymbidium sinensis, J. Fac. Agr., Kyushu Univ. 50: 35-40. Huang, C. L., and H. Okubo. 2005b. In vitro morphogenesis from rhizomes of Cymbidium sinensis. J. Fac. Agr., Kyushu Univ. 50: 11-18. Lee, Y. I., and N. Lee. 2005. Embryo development of Cypripedium formosanum in relation to seed germination in vitro. J. Amer. Soc. Hort. Sci. 130(5): 747-753. Lee, Y. I., C. F. Lu, M. C. Chung, E. C. Yeung, and N. Lee. 2007. Developmental changes in endogenous abscisic acid concentrations and asymbiotic seed germination of terrestrial orchid, Calanthe tricarinata Lindl. J. Amer. Soc. Hort. Sci. 132(2): 246-252. Lo, S. F., S. M. Nalawade, C. L. Kuo, C. L. Chen, and H. S. Tsay. 2004. Asymbiotic germination of immature seeds, plantlet development and ex vitro establishment of plants of Dendrobium tosaense Makino – a medicinally important orchid. In Vitro Cell. Dev. Biol. Plant 40: 528-535. Morse, D. H. 1987. Roles of pollen and ovary age in follicle production of the common milkweed Asclepias syriaca. Am. J. Bot. 74: 851-856. Murashige T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473–479. Paek, K. Y., and H. N. Murthy. 2002. Temperate oriental Cymbidium species. In T. Kull and J. Arditti (eds.), Orchid biology: Reviews and perspectives, VIII. Kluwer Academic Publishers, Netherlands. pp. 235-286. Proctor, H. C. 1998. Effect of pollen age on fruit set, fruit weight, and seed set in three orchid species. Can. J. Bot. 76: 420-427. Sauleda, R. P. 1976. Harvesting times of orchid seed capsules for the green pod culture process. Amer. Orchid Soc. Bull. 45: 305-309. Shiau, Y. J., A. P. Sagare, U. C. Chen, S. R. Yang, and H. S. Tsay. 2002. Conservation of Anoectochilus formosanus Hayata by artificial cross-pollination and in vitro culture of seed. Bot. Bull. Acad. Sinica 43: 123-130. Shiau, Y. J., S. M. Nalawade, C. N. Hsai, and H. S. Tsay. 2005. Propagation of Haemaria discolor Via in vitro seed germination. Biol. Plant. 49(3): 341-346. Su, H. J. 2000. Orchidaceae. In Flora of Taiwan, 2nd ed. Vol. 5 pp. 831–833. Department of Botany, National Taiwan University, Taipei, Taiwan. Thomas, T. D., and A. Michael. 2007. High-frequency plantlet regeneration and multiple shoot induction from cultured immature seeds of Rhynchostylis retusa Blume., an exquisite orchid. Plant Biotechnol. Rep. 1:243-249. Van Waes, J.M.,and P.C. Debergh. 1986. In vitro germination of some Western European orchids. Physiol. Plant. 67: 253–261. Yamato, M., and K. Iwase. 2008. Introduction of asymbiotically propagated seedlings of Cephalanthera falcata (Orchidaceae) into natural habitat and investigation of colonized mycorrhizal fungi. Ecol. Res. 23(2): 329-337. Yamazaki, J., and K. Miyoshi. 2006. In vitro asymbiotic germination of immature seed and formation of protocorm by Cephalanthera falcata (Orchidaceae). Ann. Bot. 98: 1197-1206. Yeung, E. C., S. Y. Zee, and Z. L. Ye. 1994. Embryology of Cymbidium sinensis: ovule development. Phytomorphology 44: 55-63. Yeung, E. C., S. Y. Zee, and X. L. Ye. 1996. Embryology of Cymbidium sinensis: embryo development. Ann. Bot. 78: 105-110.
摘要: 
黃道報歲蘭 (Cymbidium sinensis ‘Huang-dao’)花開放7天內的柱頭與花粉進行授粉時可獲得70 %以上的著果率,且授粉後150天齡的種子含胚率高達93 %以上。黃道報歲蘭的種子在授粉後第24週時長度達到1178 μm,胚於20週起長度開始增加,至32週時達206 μm。16週齡以前的種子完全不發芽,直至第18週時才具有發芽的能力。黃道報歲蘭的胚於第20週時已具圓球型的形態且種子含水量開始降低。培養至24週時,32週齡的種子發芽率較高,達7.9 %。150天齡的種子,1天齡 × 1天齡與3天齡 × 3天齡 (柱頭 × 花粉)的組合發芽數較多,分別為4.6與5.2 %,但授粉處理對於224天齡種子完全沒有影響。授粉後不同採收時期與授粉實驗有相同的趨勢,即較幼年的種子培養初期發芽速度較慢,但後期會大幅提升發芽率且根莖形成率較高,而較成熟的種子初期的發芽速度快,但後期根莖形成率較低。
本實驗選用23個不同葉形、葉色、花色與花形的報歲蘭品種進行性狀調查,包括植株性狀12項、花莖性狀10項與小花性狀34項,共56個項目。報歲蘭在株型上有大、中、小3級,葉姿亦有直立、斜出與下垂3種,另外有花形變異品種外,也有花色明亮的品種。無菌播種35種報歲蘭品種,各品種的發芽率從0–27.7 %不等,發芽較早且發芽率較高的品種依序為瑞玉報歲蘭、大勳報歲蘭及兩個不同來源的玉觀音報歲蘭,培養第48週時發芽率分別為27.7 %、17.8 %、13.9 %和11.8 %。

Using stigma and pollen within 7 days of flowering (DAF) could increase fruit set percentage by over 70%. Sample seeds indicated an embryo content exceeding 93%. The seeds length was 1178 μm at 24 weeks after pollination (WAP). Embryo size increased from 20 WAP, and was 206 μm at 32 WAP. In different maturity, the seeds did not germinate before 16 WAP until to 18 WAP. The embryo was globular at 20 WAP, and the water content of seeds began to decrease. The highest germination percentage was 7.9 % of 32 WAP seeds at 24 weeks culture. The germination percentages of 1 day × 1 day and 3 day × 3 day (stigma × pollen; days after flowering) at 150 DAP were 4.6 and 5.2 %, respectively. This percentage was higher than for other combinations, but seeds exhibited no effect at 224 days after pollination (DAP). In conclusion, experiments regarding the maturity and pollination of stigma and pollen exhibited similar tendencies. Younger seeds germinate slowly during early culture, but germination speed significantly increased and more rhizomes formed in later culture. In contrast, older seeds germinate quickly during early culture, but formed less rhizomes in later culture.
This experiment used 23 accessions for character examination. It included 12 items related to plant characteristics, 10 items for stalks and 34 items for flowers. Plants were separated based on height into three groups, large, midium and small. Additionally, leaves could have three different attitudes, upright, tilted, and drooping. Moreover, it had variety of exceptional shapes and colors of flowers. Compared with variation in stripes patterns on leaves, these characteristics could retained or led into later generation by sexual propagation. It tallied with commercial demand following continuous improvement and creation. In all 35 varieties, germination percentages varied between 0-27.7 %. The earlier and higher germination percentages occurred in Cym. sinensis ‘Ruei-yu', Cym. sinensis ‘Da-syun',and Cym. sinensis ‘Yu-guan-yin' I and II, and were 27.7 %, 17.8 %, 13.9 %, and 11.8 %, respectively.
URI: http://hdl.handle.net/11455/28966
其他識別: U0005-1512200815373400
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