Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/28976
DC FieldValueLanguage
dc.contributor高建元zh_TW
dc.contributor.advisor倪正柱zh_TW
dc.contributor.author張靜誼zh_TW
dc.contributor.authorChang, Ching-Yien_US
dc.contributor.other中興大學zh_TW
dc.date2009zh_TW
dc.date.accessioned2014-06-06T07:31:20Z-
dc.date.available2014-06-06T07:31:20Z-
dc.identifierU0005-2108200822003400zh_TW
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Matsuta. 2001. SSRs isolated from apple can identify polymorphism and genetic diversity in pear. Theor Appl Genet. 102:865-870. Yamamoto, T. and T. Hayashi. 2002. New root-knot nematode resistance genes and their STS markers in peach. Sci. Hort. 96:81-90. Yamamoto, T., M. Yamaguchi and T. Hayashi. 2005. An integrated genetic linkage map of peach by SSR, STS, AFLP and RAPD. J. Japan. Soc. Hort. Sci. 74 (3):204-213. Zietkiewicz, E., A. Rafalski and D. Labuda. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchhored polymerase chain reaction amplication. Genome. 20:176-183.zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/28976-
dc.description.abstract桃(Prnuns persica(L.)Batsch)為常見之落葉果樹,品種眾多,世界第四大落葉果樹。為台灣重要經濟果樹。除了以外表型態特徴為標誌外,應尋找更簡便的方法,應用在育種工作及品種分類分群上。近年來,以PCR為基礎的DNA分子標誌( DNA molecular marker)技術被廣泛應用在生物種間、種內、族群間及族群內的研究,ISSR分子標誌由於具有簡便、穩定、DNA多型性高等優點,多應用在遺傳歧異度分析、指紋圖譜分析及基因圖譜等分析。本研究以26個桃品系(包含22個由農業試驗所選育之栽培品系)為試驗材料。利用ISSR分子標誌進行不同品系遺傳相似性之評估及建立DNA指紋圖譜。藉由DNA條帶圖譜的建立,可利用單一引子區分試驗的26個品系。試驗結果以21條可擴增較多多型性條帶且再現性良好之引子,共產生170個條帶,93條為多型性條帶,多型性條帶比例為54.71%。從UBC-881(720bp)及UBC-889(700bp)引子篩選到具品種專一的二個特殊條帶,其代號為''5-9''之品種所特有。本論文之研究,顯示ISSR分子標誌應用在桃品種(系)之鑑定為有效之分析工具。zh_TW
dc.description.abstractPeach (Prnuns persica (L. ) Batsch ) is a common desiduous fruit tree. It is the fourth largest fruit trees in the world. It is also an important economic plants in Taiwan. Therefore besides morphological characteristics, it is desirable to find other markers which can be obtained easily and used in breeding and in estimating relationships among cutivars. In recent years, DNA molecular markers have been widely used in the interspecific, intraspecific, interpopulational and intrapopulational sudies. ISSR molecular marker has many advantages, including easily, stable, and polymorphism, and is often utilized in genetic diversity analysis, DNA fingerprinting, and genetic mapping. In this study, twenty-six peach cultivars (lines) including 21 hybrids(breeded DNA fingerprinting and assessment of their genetic relationship. The 26 peach cultivars(lines)could be distinguished from each other with just one primer. Twenty-one ISSR primers generated 170 fragments, 93 were polymorphic bands(polymorphism 54.71%). Two specific fragment, UBC-881(720bp)and UBC-889(700 bp), were observed in peach line ''5-9''. In this study suggested that ISSR molecular marker is a reliable tool for the fingerprinting and assessment of the genetic relationship of peach cultivars(lines).en_US
dc.description.tableofcontents中文摘要-------------------------------------------------i 英文摘要-------------------------------------------------ii 目錄 ----------------------------------------------------iii 壹、前言--------------------------------------------------------- 1 貳、前人研究 一. 桃概說--------------------------------------------------- 3 二. 品種鑑定之技術及其發展----------------------------------- 4 三. 分子標誌在桃之研究發展 (一)同功異構酶在桃之應用-------------------------------- 10 (二)DNA分子標誌技術在桃之發展應用---------------------- 11 參、材料及方法 一. 植物材料 ------------------------------------------------ 13 二. 試驗頊目及方法 (一)DNA萃取及定量-------------------------------------- 14 (二)ISSR分析------------------------------------------ 15 肆、結果 一. ISSR分析與條帶記錄--------------------------------------- 17 二. DNA條帶圖譜之建立---------------------------------------- 19 三. ISSR群叢分析-------------------------------------------- 21 伍、討論 ------------------------------------------------------- 73 陸、參考文獻---------------------------------------------------- 77 柒、附錄-------------------------------------------------------- 84 表 目 次 頁次 表一、桃種原之代號及其形態特性。-------------------------------------------------- 13 表二、ISSR分析引子序列及產生總條帶數。---------------------------------------- 23 表三、桃品種以UBC-807為引子之ISSR分析圖譜。----------------------------- 25 表四、桃品種以UBC-809為引子之ISSR分析圖譜。----------------------------- 27 表五、桃品種以UBC-811為引子之ISSR分析圖譜。----------------------------- 29 表六、桃品種以UBC-813為引子之ISSR分析圖譜。----------------------------- 31 表七、桃品種以UBC-815為引子之ISSR分析圖譜。----------------------------- 33 表八、桃品種以UBC-816為引子之ISSR分析圖譜。----------------------------- 35 表九、桃品種以UBC-823為引子之ISSR分析圖譜。----------------------------- 37 表十、桃品種以UBC-825為引子之ISSR分析圖譜。----------------------------- 39 表十一、桃品種以UBC-828為引子之ISSR分析圖譜。--------------------------- 41 表十二、桃品種以UBC-829為引子之ISSR分析圖譜。-------------------------- 43 表十三、桃品種以UBC-840為引子之ISSR分析圖譜。-------------------------- 45 表十四、桃品種以UBC-845為引子之ISSR分析圖譜。-------------------------- 47 表十五、桃品種以UBC-853為引子之ISSR分析圖譜。-------------------------- 49 表十六、桃品種以UBC-854為引子之ISSR分析圖譜。-------------------------- 51 表十七、桃品種以UBC-866為引子之ISSR分析圖譜。-------------------------- 53 表十八、桃品種以UBC-880為引子之ISSR分析圖譜。-------------------------- 55 表十九、桃品種以UBC-881為引子之ISSR分析圖譜。-------------------------- 57 表二十、桃品種以UBC-888為引子之ISSR分析圖譜。-------------------------- 59 表二十一、桃品種以UBC-889為引子之ISSR分析圖譜。----------------------- 61 表二十二、桃品種以UBC-890為引子之ISSR分析圖譜。----------------------- 63 表二十三、桃品種以UBC-891為引子之ISSR分析圖譜。----------------------- 65 表二十四、26桃品種ISSR分析之DNA指紋圖譜。------------------------------ 66 表二十五、第一群雜交組合之7品系(''90-35C'' × ''Premie''r及F1子代)以ISSR分析全部增殖片段所得相似矩陣。-------------------------------------- 67 表二十六、第二群雜交組合之5品系(''12-3'' × ''Tropic Beauty''及F1子代)以ISSR分析全部增殖片段所得相似矩陣。--------------------------------68 表二十七、第三群雜交組合之7品系(''12-3'' × ''UFGold''及F1子代)以ISSR分析全部增殖片段所得相似矩陣。---------------------------------------69 表二十八、第四群雜交組合之3品系(''66'' × ''UFGold'' 及F1子代)以ISSR分析全部增殖片段所得相似矩陣。------------------------------------70 表二十九、第五群雜交組合之4品系(''5-9'' × ''UFGold'' 及F1子代)以ISSR分析全部增殖片段所得相似矩陣。--------------------------------------71 表三十、第六群雜交組合之5品系(''5-9'' × ''Tropic Beauty''及F1子代)以ISSR分析全部增殖片段所得相似矩陣。------------------------------------72 圖 目 次 圖一、桃品種以UBC-807為引子產生之ISSR電泳圖譜。------------------------24 圖二、桃品種以UBC-809為引子產生之ISSR電泳圖譜。------------------------26 圖三、桃品種以UBC-811為引子產生之ISSR電泳圖譜。------------------------28 圖四、桃品種以UBC-813為引子產生之ISSR電泳圖譜。-------------------------30 圖五、桃品種以UBC-815為引子產生之ISSR電泳圖譜。------------------------32 圖六、桃品種以UBC-816為引子產生之ISSR電泳圖譜。------------------------34 圖七、桃品種以UBC-823為引子產生之ISSR電泳圖譜。-------------------------36 圖八、桃品種以UBC-825為引子產生之ISSR電泳圖譜。------------------------38 圖九、桃品種以UBC-828為引子產生之ISSR電泳圖譜。-------------------------40 圖十、桃品種以UBC-829為引子產生之ISSR電泳圖譜。------------------------42 圖十一、桃品種以UBC-840為引子產生之ISSR電泳圖譜。---------------------44 圖十二、桃品種以UBC-845為引子產生之ISSR電泳圖譜。---------------------46 圖十三、桃品種以UBC-853為引子產生之ISSR電泳圖譜。---------------------48 圖十四、桃品種以UBC-854為引子產生之ISSR電泳圖譜。---------------------50 圖十五、桃品種以UBC-866為引子產生之ISSR電泳圖譜。---------------------52 圖十六、桃品種以UBC-880為引子產生之ISSR電泳圖譜。---------------------54 圖十七、桃品種以UBC-881為引子產生之ISSR電泳圖譜。----------------------56 圖十八、桃品種以UBC-888為引子產生之ISSR電泳圖譜。---------------------58 圖十九、桃品種以UBC-889為引子產生之ISSR電泳圖譜。---------------------60 圖二十、桃品種以UBC-890為引子產生之ISSR電泳圖譜。---------------------62 圖二十一、桃品種以UBC-891為引子產生之ISSR電泳圖譜。------------------ 64 圖二十二、第一群雜交組合7品系(''90-35C'' × ''Premier''及F1子代)以ISSR群叢分析樹狀圖。-----------------------------------------------------------67 圖二十三、第一群雜交組合之5品系(''12-3'' × ''Tropic Beauty''及F1子代)以ISSR群叢分析樹狀圖。----------------------------------------------------68 圖二十四、第三群雜交組合之7品系(''12-3'' × ''UFGold''及F1子代)以ISSR群叢分析樹狀圖。----------------------------------------------------69 圖二十五、第四群雜交組合之3品系(''66'' × ''UFGold''及F1子代)以ISSR群叢分析樹狀圖。----------------------------------------------------70 圖二十六、第五群雜交組合之4品系(''5-9'' × ''UFGold''及F1子代)以ISSR群叢分析樹狀圖。----------------------------------------------------71 圖二十七、第六群雜交組合之5品系(''5-9'' × ''Tropic Beauty''及F1子代)以ISSR群叢分析樹狀圖。----------------------------------------------------27zh_TW
dc.language.isoen_USzh_TW
dc.publisher園藝學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2207200811222300en_US
dc.subjectPeachen_US
dc.subjectzh_TW
dc.subjectISSRen_US
dc.subjectFingerprintingen_US
dc.subject簡單序列重複區間zh_TW
dc.subject指紋圖譜zh_TW
dc.title桃的ISSR指紋圖譜分析zh_TW
dc.titleInter-Simple Sequence Repeat Fingerprinting for Peach Cultivars(Prunus perscia (L.) Bastch)en_US
dc.typeThesis and Dissertationzh_TW
item.grantfulltextnone-
item.openairetypeThesis and Dissertation-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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