Please use this identifier to cite or link to this item:
標題: 麒麟花利用花序培殖體之微體繁殖
Micropropagation of Euphorbia milii Desmoul via Cyathium Explants
作者: 卜莎蒂
Tunjit, Phusadee
關鍵字: Euphorbia milii;麒麟花;micropropagation;inflorescence;微體繁殖;花序
出版社: 園藝學系所
引用: Airo, M., G.V. Zizzo, and B. Ruffoni. 2007. In vitro propagation of an Euphorbia milii hybrid. Acta Hotr. 748: 241-246. Alvard, D., F. Côte, and C. Teisson. 1993. Comparison of methods of liquid medium culture for banana micropropagation. Effects of temporary immersion of explants. Plant Cell Tiss. Org. Cult. 32: 55-60. Amomarco, J. B. and M. R. Ibanez. 1998. Micropropagation of Limonium cavanillesii Erben, a threatened statice, from inflorescence stems. Plant Growth Reg. 24: 49-54. Anderson, H.M., A. J. Abbott, and S. Wiltshire. 1982. Micropropagation of strawberry plants in vitro - Effect of growth regulators on incidence of multi-apexing abnormality. Sci. Hortic. 16: 331-341. Aswath, C. R. and M. L. Choudhary. 2002. Rapid plant regeneration from Gerbera jamesonii bolus callus cultures. Acta Bot. Croat. 61: 125-134. Badzian, T., G. R. Hennen, and J. Fotyma-Kern. 1991. In vitro rooting of clonal propagated miniature rose cultivars. Acta Hort. 289:329-330. Bag, N., S. Chandra, L. M. S. Palni, and S. K. Nandi. 2000. Micropropagation of Dev-ringal (Thamnocalamus Spathiflorus (Trin) Munro) a temperate bamboo, and comparison between in vitro propagated plants and seedling. Plant Science. 156: 125-135. Berthouly, M. and H. Etienne. 2002. Temporary immersion system: A new concept for use liquid medium in mass propagation. 1st International Symposium on Liquid Systems for in vitro Mass Propagation of Plants. Cost 843 Working Group. Aas Norway. P. 37-38. Berthouly, M. and H. Etienne. 2002. Temporary immersion systems in plant micropropagation. Plant Cell .Tiss. Org. Cult. 69: 215-231. Bressan, P. H., Y. J. Kim, S. E. Hyndman, P. M. Hasegawa, and R. A. Bressan. 1982. Factors affecting in vitro propagation of rose. J. Am. Soc. Hort. Sci. 107: 979- 990. Cheng, B., C. M. Peterson and R. J. Mitchell. 1992. The role of sucrose, auxin and explant source on in vitro rooting of seedling explants of Eucalyptus sideroxylon. Plant Sci. 87: 207-214. Chu, C. Y. 1991. The efficient propagation method of miniature roses. Doctor's thesis of Philosophy in Horticulture. Urbana-Champaign. Graduate College of University of Illinois. 113 pp. CITES. 2004. The convention on international trade in endangered species of wild fauna and flora. Consideration of proposals for amendment of appendices I and II. Bangkok. The thirteenth Conference of the Parties 2 - 14 October 2004. ( eng/cop /13/prop/E13-P39.pdf) Davies, D. R. 1980. Rapid propagation of roses in vitro. Sci. Hortic. 13: 385-389. Debergh, P. C., J. Aitken-Christie, D. Cohen, B. Grout, S. Von Arold, R. Zimmerman and M. Ziv. 1992. Reconsideration of the term ‘Vitrification' as used in micro- propagation. Plant Cell Tiss. Org. Cult. 30: 135-140. Debergh, P.C. and P.E. Read. 1991. Micropropagation. In: Debergh, P.C.and R.H. Zimmerman (eds.). Micropropagation Technology and Application. Kluwer Academic Publishers, Dordrecht Boston - London. P. 1-13. Dewir, Y. H., D. Chakrabarty, E. J. Haen and K.Y. Paek. 2005. Reversion of inflorescence development in Euphorbia millii and its application to large-scale micropropagation in an air-lift bioreactor. J. Hort. Sci. Bio. 80 (5):581-587. Dibanuka, C. 2006. Amazing Thai crown of thorns. Royal Flora Ratchaphruek 2006, Horticultural Research Institute (HRI)-Department of agriculture (DOA), and Society for Ornamental Plants of Thailand (SOPT). Bangkok. 20 pp. Douglas, G. C., C. B. Rutledge, A. D. Casey, and D. H. S. Richardson. 1989. Micropropagation of floribunda, ground cover and miniature roses. Plant Cell Tiss. Org. Cult. 19: 55- 64. Dubois, L. A. M., J. Roggemans, G. Soyeurt, and D. P. De Vries. 1988. Comparison of the growth and development of dwarf rose cultivars propagated in vitro and in vivo by soft cuttings. Sci Hort. 35: 293-299. EPI: Economics price index of Taiwan. (From 1990-May 2008 = 1.34) http://www. /public/ data/dgbas03/bs3/inquire/cpispl.xls Escalona, M., J.C. Lorenzo, B. Gonzàlez, M. Daquinta, J.L. Gonzàlez, Y. Desjardins and C.G. Borroto. 1999. Pineapple (Ananas comosus L. Merr) Micropropagation in Temporary Immersion Systems. Plant Cell Rep. 18: 743-748. Feyissa, T., M. Welander, and L. Negash. 2007. Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica. Biologia Plantarum. 51(1): 15-21. Fosket, D. E. 1994. Plant Growth and Development: a Molecular Approach. Academic Press Limited. San Diego, New York, Boston, London, Sydney, Tokyo - Toronto. 580 pp. Gonçalves, J. C., G. Diogo, and S. Amâncio. 1998. In vitro propagation of chestnut (Castanea sativa×C. crenata): Effects of rooting treatments on plant survival, peroxidase activity and anatomical changes during adventitious root formation. Sci Hort. 72 (3-4): 265-275. Gonçalves, S. and, A. Romano. 2007. Ex vitro rooting of Drosophyllum lusitanicum micropropagated shoots improves acclimatization. Acta Hort. 748: 241-246. Grigoriadou, K., M. Vasilakakis, and E. P. Eleftheriou. 2003. Rooting and acclimatization of in vitro propagated olive tree microshoots (Olea europaea sativa L.) cv. Chondrolia Chalkidikis. Acta Hort. 616: 193-197. Hahn, E.J. and K.Y. Paek. 2005. Multiplication of chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stem. Plant Cell Tiss. Org. Cult. 81: 301-306. Hartmann, T. H., D. E. Keater, F. T. Davies, Jr., and R. L. Geneve. 2002. Plant Propagation: Principles and Practices. 2nd eds. New Jersey. Prentice Hall Publisher. 880 pp. Hasegawa, P.M. 1980. Factors affecting shoot and root initiation from cultured rose shoot tips. J. Am. Soc. Hortic. Sci. 105(2): 216- 220. Hatzilazarou, S. and C. Ttooulos. 2003. In vitro and ex vitro rooting and plantlet acclimatization in Nerium oleander. Acta Hort. 616: 221-225. Hatzilazrou, S., M. Kotis, A. S. Economou and N. Rifaki. 2003. Rooting of microcuttings and acclimatization in Bougainvillea. Acta Hort. 616: 265-269. Hatzilazarou, S., C. Ttooulos, A.S. Economou, N. Rifaki, and P. Ralli. 2003. In vitro and ex vitro rooting and plantlet acclimatization in Nerium Oleander. Acta Hort. 616: 221-226. Heloir, M. J. C. Fournioux, L. Oziol, and R. Bessis. 1997. An improved procedure for the propagation in vitro of grapevine (Vitis vinifera cv. Pinot noir) using axillary-bud microcuttings. Plant Cell .Tiss. Org. Cult. 49: 223-225. Huang, F. H., J. M. Al-Khayri, and E. E. Gbur. 1994. Micropropagation of Acaia mearnsii. In Vitro Cell. Dev. Biol. 30: 70-74. Huang, M. C. and C. Y. Chu. 1985. Ascheme for commercial multiplication of gerbera (Gerbera hybrida Hort.) through shoot tip culture. J. Japan. Soc. Hort. Sci. 54 (1): 94-100. Hussey, G. 1976a. In vitro release of axillary shoots from apical dominance in monocotyledonous plantlets. Ann. Bot. 40:1323-1325. Hvoslef-Eide, A. K., C. Munster, P. H. Heyerdahl, R. Lyngved, and O. A. S. Olsen. 2003. Liquid culture system for plant propagation. Acta Hort. 625: 173-183. Illg, R.D. and R. T. Faria. 1995. Micropropagation of Alpinia purpurata from inflorescence bubs. Plant Cell Tiss. Org. Cult. 40:183-185. Jabbarzadeh, Z. and M. K. Khui. 2005. Factors affecting tissue culture of Damask rose (Rosa damascena Mill.). Sci Hort. 105: 475-482. Kaçar, Y. A., M. Mazmanoğlu, Y. Y. Mendi, S. Serçe and S. Çetiner. 2005. The effect of cytokinin type and concentration on multiplication rate of Spathiphyllum (Fam. Araceae). Asian J. Plant Sic. 4(4): 401-404. Khosh-khui M. and K. C. Sink. 1982. Rooting enhancement of Rosa hybrida for tissue culture propagation. Sci. Hort. 17: 371-376. Kim, M.S., N.B. Klopfenstein, and B.M.Cregg. 1998. In vitro and ex vitro rooting of micropropagated shoots using three green ash (Fraxinus pennsylvanica) clones. New Forests. 16 (1): 43-57. Kitin, P., I. Iliev., A. Scaltsoyiannes., C. Nellas, A. Rubos., and R. Funada. 2005. A comparative histological study between normal and fasciated shoots of Prunus avium generated in vitro. Plant Cell Tiss. Org. Cult. 82: 141-150. Lane, D.W. 1978. Regeneration of apple plants from shoot meristem. Plant Sci. Lett. 13: 281-285. Leonardi, C., R. Antoninio, and M. Stefano la. 2001. Hormone effects on in vitro proliferation rooting of Grevillea explants. Sci Hort. . 90: 335-341. Luna C., P. Sansberro, L. Mroginski, and J. Tarragó. 2003. Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system. Biocell. 27(2): 205-212. Nakano, M., Y. Niimi, D. Kobayashi, and A. Watanabe. 1999. Adventitious shoot regeneration and micropropagation of hybrid tuberous begonia (Begonia x tuberhybrida Voss). Sci Hort. . 79: 245-251. Nas, M. N. and P. E. Read. 2003. Ex vitro survival of hybrid hazelnut shoots produced in vitro. Acta Hort. 616: 215-219. Neeta, D. S., L. George, and S. Eapen. 2000. Direct regeneration of shoot from immature inflorescence cultures of turmeric. 62:235-238. Maesato, K., K. S. Sarma, H. Fukui, and T. Hara. 1991. In vitro bulblet induction from shoot apices of Lilium japonicum. HortSci. 26: 221. Marks, T. R. and T. R. Simpson. 1994. Factor affecting shoot development in apically dominant Acer cultivars in vitro. J. Hortic. Sci. 69: 537-543. Martin, K. P. 2003. Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep. 21(5): 415-420. Martin, K. P., M. R. Beena and, D. Joseph. 2003. High frequency axillary bud multiplication and ex vitro rooting of Wedelia chinensis (Osbeck) Merr. a medicinal plant. Indian J. Exp. Biol. 41(3): 262-266. Mauseth, J. D. 2003. Botany: an Introduction to Plant Biology. 3rd ed. Jones and Bartlett Publishers. Sudbury, Mississauga and London. 580 pp. McClelland, M. T., M. A. L. Smith and Z. B. Carothers. 1990. The effects of in vitro and ex vitro root initiation on subsequent microcutting root quality in three woody plants. Plant Cell Tiss. Org. Cult. 23(2): 115-123. McLaughlin, J. and J. Garofalo. 2002. Crown-of-Thorns, Euphorbia milii production & landscape use. Miami Dade. University of Florida. Cooperative extension service. 2 pp. M'Kaba, J., N. Dorion, and C. Bigot. 1991. In vitro propagation of Cistus x purpureus Lam. Sci Hort. 46:155-160. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physio. Plant. 15: 473-497. Offord , C. A. and L. C. Campbell. 1992. Micropropagation of Telopea speciosissima R. Br. (Proteacae). 2: Rhizogenesis and acclimatization ex vitro conditions. Plant Cell Tiss. Org. Cult. 29: 223-230. Pati, P. K., S. P. Rath, M. Sharma, A. Sood, and P. S. Ahuja. 2006. In vitro propagation of rose-a review. Biotechnology Advances. 24: 94-114. Peeters, A. J. M., W. Gerads., G. W. M. Barendse, and G. J. Wullems. 1991. In vitro flower bud formation in tobacco: interaction of hormones. Plant Physiol 97: 402-408. Pierik, R. L. M., H. H. M. Steegmans, A. A. elias, O. T. J. Stiekema, and A. J. ven der Velde. 1988. Vegetative propagation of Syringa vulgaris L. in vitro. Acta Hort. 226: 195-204. Pierik, R. L. M., A. van Voorst, G. Boooy, C. A. M. van Acker, C. L. C. Lelivelt, and J. C. de Wit. 1988. Vegetative propagation of Alstroemeria hybrids in vitro. Acta Hort. 226: 81-89. Poornima, G. N. and V. Ravishankar Rai. 2007. In vitro propagation of wild yams, Dioscorea oppositifolia (Linn) and Dioscorea pentaphylla (Linn). Afr. J. Biotechnol. 6: 2348-2352. Pospíšilová, J., I. Tichá, P. Kadleček, D. Haisel, and Š. Plzáková. 1999. Acclimatization of micropropagated plants to ex vitro conditions. Biologia Plantarum. 42: 481-497. Preece, J.E., and E.G. Sutter. 1991. Acclimatization of micropropagated plants to the greenhouse and field. In: Debergh, P.C., Zimmerman, R.H. (ed.): Micropropagation Technology and Application. Kluwer Academic Publishers, Dordrecht - Boston - London. p. 71-93. Purohit S. D. and A. Singhvi. 1998. Micropapragation of Achras sapota through enhanced axillary branching. Sci. Hort. 76: 219-229. Ripley, K. and J. E. Preece. 1986. Micropropagation of Euphorbia lathyris L. Plant Cell. Tiss. Org. Cult. 5: 213-218. Roels, S., M. Escalona, I. Cejas, C. Noceda, R. Rodriguez, M.J. Canal, J. Sandoval, and P. Debergh. 2005. Optimization of plantain (Musa AAB) micropropagation by temporary immersion system. Plant Cell Tiss. Org. Cult. 82: 57-66. Rodrigues, P. H. V., A. M. L. P. Lima, G. M. B. Ambrosano, M. F. B. Dutra. 2005. Acclimatization of micropropagated Heliconia bihai (Heliconiaceae) plants. Sci. Agric. (Piracicaba, Braz.). 62: 299-301. Rout, G.R., A. Mohapatra, and S. Mohan Jain. 2006. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnology Advances. 24: 531-560. Singh, N. K. and C. B. Sehgal. 1999. Micropropagation of ‘Holy Basil' (Ocimum sanctum Linn.) from young inflorescences of mature plants. Plant Growth Regul. 29: 161-166. Singh, F. and D. Prakash. 1984. In vitro propagation of Thunia alba (Wall.) Reichb. f. through flower stalk cuttings. Sci Hort. 24: 385-390. Taed, A. G. and P. G. Alderson. 1987. Micropropagation of tulip: optimizing shoot propagation from floral stem explants. Acta Hort. 212: 677-681. Taiz, L. and E. Zeiger. 2002. Growth and development: the control flowering. In: Taiz, L. and E. Zeiger (eds.). Plant Physiology. Sinauer Associates Inc Publishers, Sunderland. P. 559-590. Takayama, S., M, Akita. 1994. The Type of Bioreactor used for Shoot and Embryos. Plant Cell Tiss. Org. Cult. 39: 147-156. Tanaka, M. and Y. Sakanishi. 1978. Factors affecting the growth of in vitro culture lateral buds from Phalenopsis flower stalks. Sci Hort. 8:169-178. Toogood, A. 2003. Propagating Plants. 2nd ed. London. The Royal Horticulture Society. A Dorling Kindersley Book Publisher. p. 246. Tomsone, S., A. Galeniece, A. Akere, G. Priede, L. Zira. 2007. In vitro propagation of Syringa vulgaris L. cultivas. Biologija. 53: 28-31. Topoonyanont, N. 2003. Plant propagation by tissue culture. Project for propagation of economic crops. Chaimai. Maejo University. 163 pp. Topoonyanont, N., S. Chongsang, S. Chujan, S. Somsueb, and P. Nuamjareen. 2005. Micropropagation Scheme of Curcuma alismatifolia Gragnep. Acta Hort. 673: 705-712. Topoonyanont, N., R. Ampawan, and P. C. Debergh. 1999. Bushiness in Gerbera jamesonii: abnormal shoot development during micropropagation. J. Hortic Sci. Biotech. 74: 675-679. Topoonyanont, N., R. Ampawan and P.C. Debergh. 2000. Reversion of Limonium hybrid ‘Misty Blue' inflorescence development and its applicability in micropropagation. Sci Hort. 83: 283-299. Topoonyanont, N., R. Ampawan, L. Phetpradap, and Y. Paper. 1996. Research and improvement of Gerbera propagation by using tissue culture for agriculture commercial. Bangkok. (Report of National Ornamental Symposium). p. 135-141. Van der Krieken, W.M., H. Breteler, M. H. M. Visser, and D. Mavridou. 1993. The role of conversion of IBA into IAA on root regeneration in apple: introduction of a test system. Plant Cell Rep. 12: 203-206. Wildi, E., W. Schaffner, and K. B. Buter. 1998. In vitro propagation of Petasites hybridus (Asteraceae) from leaf and petiole explants and from inflorescence buds. Plant Cell Rep. 18: 336-340. Xu, J., Y. Wang, Y. Zhang, and T. Chai. 2008. Rapid in vitro multiplication and ex vitro rooting of Malus zumi (Matsumura) Rehd. Acta Physiologiae Plantarum. 30: 129-132. Ziv, M. 2002. Simple bioreactors for mass propagation plant. 1st International Symposium on Liquid Systems for in vitro Mass Propagation of Plants. Oslo. p. 13-14. Watad, A. A., J. Ben-Jaacov, E. Tal, H. J. Solomom. 1992. In vitro propagation of Grevillea species. Acta Hort. 316: 51-53. Wongsuwan, C. 1996. Euphorbiaceae. 1st ed. Bangkok. Pat Book Co., Ltd. 128 pp. Wulster, G. and J. Sacalis 1980. Effects of auxins and cytokinins on ethylene evolution and growth of rose callus tissue in sealed vessels. Hortic Sci.15: 736-737.
以花麒麟(Euphorbia milii Desmoul)易分枝型(free-branching)之紅花品種‘FBR’和難分枝型(restricted-branching)的綠花品種 ‘RBG’之大戟花序為材料,進行微體繁殖 。將‘FBR’ 和‘RBG’ 第一期大戟花序培養在含5 mg/l BA 的1/2 MS培養基中,可得較多的再生芽體。此外花麒麟 ‘FBR’ 或 ‘RBG’ 之芽體分別繼代培養在含2 mg/l BA 或 4 mg/l BA,芽體增殖數較多。另外將花麒麟 ‘FBR’ 或‘RBG’ 之芽體繼代培養在固體或液體培養基中,以固體培養基的增殖芽體數較多,芽體在液體培養時會有玻璃質化(hyperhydricity)的現象。將‘FBR’ 和 ‘RBG’之叢生芽分離成單枝,將枝梢培養在含IAA、IBA或NAA的發根培養基中,含NAA的培養基會抑制芽體的生長與發根,而以培養在無auxin的培養基中根的生長較佳。取 ‘FBR’ 和 ‘RBG’之長1.5 cm的枝梢進行瓶外發根試驗。以枝梢基部沾2 g/Kg IBA的發根劑之存活率較高。比較利用傳統的扦插或微體繁殖‘FBR’ 和 ‘RBG’的苗上盆2個月後插穗的品質。‘FBR’經微體繁殖的苗較佳,但在 ‘RBG’則無明顯差異。

Euphorbia milii Desmoul micropropagation was successful by using cyathia as explant source in two types of E. milii, free branching type with small red bract (‘FBR') and another restricted-branching type with large green bract (‘RBG'). For both cultivars, the first stage of cyathium cultured on one and half strength of MS medium containing 5 mg/l BA was more effective on vegetative shoots regeneration. The vegetative shoots obtained from cyathium in vitro were used as explants for subculture. The higher number of multiple shoots were achieved with shoots subcultured on full strength of MS medium containing 2 or 4 mg/l BA for E. milii ‘FBR' or ‘RBG', respectively. Two medium phases, semi-solid and liquid culture were compared for shoot multiplication. There were more shoots when subcultured on semi-solid medium in two E. milii cultivars, while shoots were hyperhydricity when subcultured on support with liquid medium. Multiple shoots from multiplication were divided into single shoot and cultured on pretransplanting medium supplement with IAA, IBA, or NAA. NAA was found to inhibit the growth and rooting of shoots. While, two E. milii cultivars rooted well when cultured on medium without auxin. For ex vitro rooting, shoots were harvested from in vitro culture and cut into single shoot of 1.5 cm in length and treated with auxin powder. E. milii cultivars microcutting rooted well and highest survival rate under ex vitro condition when treated with 2 g/Kg IBA powder. The microcutting size of E. milii did not influenced on rooting and survival rate. The performance of pot flower propagated by conventional cutting or micropropagation was compared. There were found ‘FBR' cultivar propagated by micropropagation were better performance than that by conventional cutting. But ‘RBG' cultivar had not differences in performance of pot plant between propagation by micropropagation or conventional cutting. The micropropagation with ex vitro rooting is a simple protocol for production of E. milii plantlets.
其他識別: U0005-2207200811222300
Appears in Collections:園藝學系

Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.