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標題: 利用形態性狀、RAPD及SCAR分子標誌分析butterhead與crisphead萵苣遺傳歧異度
Genetic Diversity in butterhead and crisphead lettuce (Lactuca sativa L.) by using Morphological Traits, Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Regions (SCAR) Markers.
作者: 蔡宗育
Tsai , Tzung-Yu
關鍵字: lettuce;萵苣;genetic diversity;RAPD;SCAR;遺傳歧異度;RAPD;SCAR
出版社: 園藝學系所
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利用植株形態、RAPD (Random Amplified Polymorphic DNA)和SCAR (Sequence Characterized Amplified Regions)三種方法進行萵苣31個crisphead形態品系及26個butterhead形態品系之遺傳歧異度研究。以19項外表性狀指數所得資料進行UPGMA群叢分析,可將57個品系區分成二個群組,遺傳相似度為0.38。第一群組中21個品系皆為crisphead品系,不僅包含全部15個台南結球萵苣品系還包含3個美國品系,第一群組可細分5個亞群,遺傳相似度在0.61以上;第二群組中包含了10個crisphead品系與26個butterhead品系,第二群組可分為6個亞群,遺傳相似度在0.70以上。

The genetic diversity of 31 butterhead (BUT) and 26 crisphead (CRP) accessions were evaluated by morphological traits, RAPD (Random Amplified Polymorphic DNA) and SCAR (Sequence Characterized Amplified Regions). Data for 19 morphological traits were subjected to a genetic diversity analysis, after which a UPGMA cluster analysis was performed. The 57 accessions were clustered into 2 groups according to type with genetic similarity of 0.38. The first group included 15 accessions form Tainan and 3 accessions form the National Germplasm of the USA, which were clustered into 5 subgroups with the similarity beyond 0.61. The second group included 10 crisphead and 26 butterhead accessions, which were clustered into 6 subgroups with the similarity beyond 0.70.
A total of 271 RAPD bands, with a mean band of 9.3 for each primer, were generated using 29 out of 130 primers in the RAPD analysis. The polymorphism was 53.9%. All accessions were clustered into 3 groups in the RAPD analysis. The first group included 13 crisphead accessions form the Taiwan agricultural research institute(TARI) with the similarity 0.86. The second group including 15 accessions form the Tainan district agricultural research and extention station(TDARES)、3 accessions form USA and 3 butterhead accessions with the similarity 0.88. The first, second and fourth subgroups included crisphead accessions. The third and fifth subgroups included 3 butterhead accessions. The third group was composed of 23 butterhead accessions with a the similarity of 0.82.
In analyzing results from SCAR of butterhead and crisphead accessions, a total of 100 bands were generated using 21 out of 51 primer pairs. The means of bands generated by a primer pairs was 4.8 and the polymorphism was 73%. The 57 accessions were clustered into 3 groups. The first group included 13 crisphead accessions form the TARI with the similarity 0.76. The second group including 15 accessions form TDARES with a similarity of 0.88. The third group was composed of 26 butterhead and 3 accessions the USA with a similarity of 0.88. The analysis of RAPD and SCAR result in the main group of the dendrogram showed a similarity of 0.78~0.86 based on RAPD markers and which the SCAR markers displayed a similarity 0.68~0.78. The analysis of RAPD and SCAR result in the subgroup of the dendrogram showed a similarity of 0.84~0.96 based on RAPD markers and which the SCAR markers displayed a similarity 0.88~0.97.
Lettuce germplasm could be identified by morphological characteristics such as leaf color, leaf texture and head type. The molecular markers could be used to cluster accessions into groups by SCAR and subgroups by RAPD successfully.
其他識別: U0005-2208200809332600
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