Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29050
標題: 芭菲爾鞋蘭之微體繁殖
The Micropropagation of Paphiopedilum
作者: 黃慧宜
Huang, Hui-I
關鍵字: Paphiopedilum;芭菲爾鞋蘭;survival rate;composition of medium;organic compound;存活率;培養基組成;有機物
出版社: 園藝學系所
引用: 王美陽、朱建鏞. 1995. 培養基物相對組織培養培植體生長的影響. 農林學報 44:71-77. 王政琳. 1997. 拖鞋蘭微體繁殖技術之建立與改進. 碩士論文. 國立 台灣大學農藝研究所. 64pp. 李秋梅. 1995. 銀葉桉微體繁殖之研究. 碩士論文. 國立中興大學園 藝研究所. 107pp. 李勇毅. 1995. 拖鞋蘭. p.923-926. 刊於農委會臺灣農家要覽增修 訂三版策劃委員會編著. 台灣農家要覽. 豐年社. 台北. 李勇毅. 1997. 拖鞋蘭分類及栽培管理. 台灣花卉園藝 117:40-43. 呂依倫、李志仁、李哖. 1992. 培養基成分對素心蘭種子無菌發芽之 影響. 中國園藝 38:161-169. 吳新棋、李哖. 1991. 紅花鶴頂蘭之無菌發芽. 中國園藝 37:183- 198. 林永浩. 1998. 芭菲爾鞋蘭與石斛蘭之組織培養. 碩士論文. 中國文 化大學生物科技研究所. p.20-30. 林文華. 2003. 淺談拖鞋蘭分類及雜交育種方向. 台灣花卉園藝 187:34-40. 洪寶瑩. 2004. 芭菲爾鞋蘭、文心蘭、軛瓣蘭及堇蜘蘭之微體繁殖. 碩士論文. 國立台灣大學園藝研究所. p.5-12 高水恩. 2006. 國際仙履蘭產業現況與我國仙履蘭外銷前景. 農業世 界. 208:52-56. 高紀清. 2008. 仙履蘭產業發展新展望. 生活蘭藝. 33:75pp. 高景輝. 1994. 植物生理荷爾蒙. 華香園. 台北. p.69-84. 許伊琍. 2007. 仙履蘭芭菲爾鞋蘭屬賞花圖鑑. 日月文化. 台北. p.43-123. 張訓堯. 2000. 營養缺乏及過量對玫瑰花生長之影響. 碩士論文. 國 立中興大學園藝研究所. 114pp. 張仁銓. 2007. 人工培植拖鞋蘭證明文件申請簡介及出口管理現況. 農政與農情. 178:54-57. 陳美惠. 1996. 果莢成熟度、種子前處理及培養基成分對拖鞋蘭無菌 播種與幼苗生長之影響. 碩士論文. 國立台灣大學園藝研究所. 76pp. 陳婷玉. 2000. 六種芭菲爾鞋蘭之組織培養. 碩士論文. 國立台灣大 學園藝研究所. 55pp. 麥奮. 1987. 拖鞋蘭之芭菲爾鞋蘭屬. 淑馨出版社. 台北. 284pp. 廖本裕. 1986. 無土花草栽培. p.41 五洲出版社. 台北. 廖曼利、李哖. 2003. 培養基成分、光線及溫度對報歲蘭根莖生長與 分化的影響. 蘭花類-一葉蘭、國蘭、拖鞋蘭、文心蘭及其他蘭 類. 台灣花卉發展協會. 台北. p.360. 蔡媦婷. 2004. 通氣性和液體培養基對蝴蝶蘭瓶苗生長之影響. 博士 論文. 國立中興大學園藝所. 170pp. 蔡瑜卿. 2006. 我國拖鞋蘭培植場登記制度及拖鞋蘭種苗出口現況. 農業世界. 280:58-62. 蔡瑜卿. 2007. 仙履蘭種苗出口現況. 仙履蘭產品發展座談會書面資 料. p.8-15. 蕭元川. 1996. 蘭界的另一顆新星-拖鞋蘭. 台灣花卉園藝. 110:42- 43. 蕭元川. 2002. 拖鞋蘭種苗產業現況及問題. 植物種苗 4:51-54. 蕭伊芸. 2002. 乳羅黛粉葉誘變種之微體繁殖與再誘變. 碩士論文. 國立中興大學園藝所. 61pp. 河瀨晃四郎. 1988. パフイオペデイルムの組織培養による增殖. J. Jpn. Soc. Hort. Sci. 57(Suppl. 1):376-377. Archibald, J.A. 1964. Wheather effects on leaf-nutrient composition of fruit crops. p.1-24. In: C. Bould , P. Prevot, and J.R. Magness.(eds.), Plant analysis and fertilizer problems, Ⅳ. W. F. Humphrey press, Inc., Geneva, New York. Arigita, L., R. Sánchez Tamés, and A. González. 2003. 1- Methylcyclopropene and ethylene as regulators of in vitro organogenesis in kiwi explants. Plant Growth Regulat. 40:59-64. Bornman, C. H. and T. C. Vogelmann. 1984. Effect of rigidity of gel medium on benzyladenine-induced adventitious bud formation and vitrification in vitro in Picea abies. Physiol. Plant. 61:505-512. Bon, M.C., M. Gendraud, and A. Franclet. 1988. Roles of phenollic compounds on micropropagation of juvenile and mature clones of Sequoiadendron giganteum: influence of activated charcoal. Scientia Hort. 34:283-291. Chen, T.Y., J.T. Chen, and W.C. Chang. 2002. Multiple shoot formatiom and plant regeneration from stem nodal explants of Paphiopedilum orchids. In Vitro Cell Dev. Biol.-plant 38:595-597. Chen, T.Y., J.T. Chen, and W.C. Chang. 2004. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell. Tiss. Org. Cul. 79:11-15. Chu, C.Y., S.L. Knight, and M.A.L. Smith. 1993. Effect of liquid culture on the growth and developmwnt of miniature rose (Rosa chinensis Jacq. ‘Minima’). Plant Cell Tiss. Org. Cult. 32:329-334. Cos Terrer, J. and D. Frutos Tomás. 2001. Determination of macronutrients to be included in in vitro culture media according to leaf concerations. J. Hort. Sci. Biotechnol. 76:484-488. Dahleen, L. S. 1995. Improved plant regeneration from barley callus cultures by increased copper levels. Plant Cell Tiss. Org. Cul. 43:267-269. Debergh, P. C. and L. J. Maene. 1981. A scheme for commercial propagation of ornamental plants by tissue culture. Scientia Hort. 14:335-345. Dimasi-Theriou, K., A. E. Economou, and E. M. Sfakiotakis. 1993. Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene. Plant Cell Tiss. Org. Cult. 32:131-136. Duran-Cresswell, R. and C. Nitsch. 1977. Factors influcing the regeneration of Eucalyptus grandis by organ culture. Acta Hort. 78:149-155. Etienne, H. and M. Berthouly. 2002. Temporary immersion systems in plant micropropagation. Plant Cell Tiss. Org. Cult. 69:215-231. Flamée, M. 1978. Influence of selected media and supplements on the germination and growth of paphiopedilum seedling. Amer. Orch. Soc. Bull. 47:419- 423. Fridborg, G., M. Pedersén, L.E. Landström, and T. Eriksson. 1978. The effect of activated charcoal on tissue cultures : adsorption of metabolites inhibiting morphogenesis. Physiol. Plant. 43:104-106. González, A., L. Arigita, J. Majada, and R. Sánchez-Tamés. 1997. Ethylene in in vitro organogenesis and plant growth of Populus tremula L. Plant Growth Regulat. 22:1- 6. Hu, H. and P. H. Brown. 1994. Localization of boron in cell walls of squash and tobacco and its association with pectin; evidence for a structural role boron in the cell wall. Plant Physiol. 105:681-689. Huang, M.C. and C.Y. Chu. 1985. A scheme for commercial multiplication of gerbera (Gerbera hybrida Hort.) through shoot tip culture. J. Jpn. Soc. Hort. Sci. 54:94-100. Huang, L.C. 1988. A procedure for asexual multiplication of paphiopedilums in vitro. Amer. Orchid Soc. Bull.57: 274–278. Huang, L.C., C.J. Lin, C.I. Kuo, B.L. Huang, and T. Murashige. 2001. Paphiopedilum cloning in vitro. Scientia Hort. 91:111-121. Iwasaki, K., K. Sakurai, and E. Takahashi. 1990. Copper binding by the root cell walls of Italian ryegrass and red clover. Soil Sci. Plant Nutr. 36:431-439. Jiang, Y., L. Pen, and J. Li. 2004. Use of citric acid for shelf life and quality maintenance of fresh-cut Chinese water chestnut. J. Food Eng. 63:325-328. Kawase, K. 1990. Clonal propagation of paphiopedilum by tissue culture. 3. Effect of flower age on PLB formation and vegetative growth of undeveloped buds. J. Jpn. Soc. Hort. Sci. 59(Suppl. 2):666-667. Kawase, K. 1994. Clonal propagation of paphiopedilum by tissue culture. 4. Formation and culture of knot-like- tissues and shoots from ovaries and flower stalks. J. Jpn. Soc. Hort. Sci. 63(Suppl. 2):514-515. Kawase, K. 1995a. Induction of embryoids in shoot apex culture of Paphiopedilum. J. Jpn. Soc. Hort. Sci. 64 (Suppl. 2):52-53. Kawase, K. 1995b. Effects of thidiazuron on shoot propagation in Paphiopedilum. J. Jpn. Soc. Hort. Sci. 64 (Suppl. 2):598-599. Kawase, K. 1997. Inforescence culture in species of Paphiopedilum. J. Jpn. Soc. Hort. Sci. 66(Suppl. 1):512- 513. Kim, E.K., E.J. Hahn, H.N. Murthy, and K.Y. Paek. 2003. High frequency of shoot multiplication and bulblet formation of garlic liquid cultures. Plant Cell Tiss. Org. Cult. 73:231-236. Kintzios, S., J.B. Drossopoulos, and C. Lymperopoulos. 2000. Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from young mature leaves of rose. J. Plant Nutr. 23:1407-1420. Kothari, S. L., K. Agarwal, and S. Kumar. 2004. Inorganic nutrient manipulation for highly improved in vitro plant regeneration in finger millet-Eleusine coracana (L.) Gaertn. In Vitro Cell. Dev. Biol.- Plant 40:515- 519. Li, C., H. Pfeffer, F. Dannel, V. Römheld, and F. Bangerth. 2001. Effect of boron starvation on boron compartmentation, and possibly hormone-mediated elongation growth and apical dominance of pea (Pisum sativum) plants. Physiol. Plant. 111:212-219. Lidon, F. C., M.da G. Barreiro, and F. S. Henriques. 1995. Interactions between biomass production and ethylene biosynthesis in copper-treated rice. J. Plant Nutr. 18:1301-1314. Lin, Y. H., C. Chang, and W.C. Chang. 2000. Plant regeneration from callus culture of a Paphiopedilum hybrid. Plant Cell. Tiss. Org. Cul. 62:21-25. Meira, Z. and G. Gadasi. 1986. Enhanced embryogenesis and plant regeneration from cucumber(Cucumis sativus L.) callus by activated charcoal in solid/liquid double- layer cultures. Plant Sci. 47: 115-122. Morard, P. and M. Henry. 1998. Optimization of the mineral composition of in vitro culture media. J. Plant Nutr. 21:1565-1576. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physi. Plant. 15:473-497. Orlikowska, T., I. Sabala, and D. Kucharska. 2000. The effect of leaf and shoot tip removal and explants orientation on axillary shoot proliferation of Codiaeum variegatum Blume var. pictum Muell. Arg. Cv. Excellent. Scientia Hort. 85:103-111. Purnhauser, L. and G.. Gyulai. 1993. Effect of copper on shoot and root regeneration in wheat, triticale, rape and tobacco tissue cultures. Plant Cell Tiss. Org. Cul. 35:131-139. Rugini, E. 1984. In vitro propagation of some olive (Olea Europaea sativa L.) cultivars with different root- ability , and medium development using analytical data from developing shoots and embryos. Scientia Hort. 24:123-134. Sato, S., M. Hagimori, and S. Iwai. 1993. Recovering vitrified carnation (Dianthus caryophyllus L.) shoots using Bacto-Peptone and its subfractions. Plant Cell Rpt. 12:370-374. SharmA, S. K. and V. Ramamurthy. 2000. Micropropagation of 4-year-old elite Eucalyptus tereticornis trees. Plant Cell Rpt. 19:511-518. Singha, S. 1982. Influence of agar concentration on in vitro shoot proliferation of Malus sp.‘Almey’and Pyrus communis ‘Seckel’. J. Amer. Soc. Hort. Sci. 107:657-660. Stavrianakou, S., G. Liakopoulos, and G. Karabourniotis. 2006. Boron deficiency effects on growth, photosynthesis and relative concentrations of phenolics of Dittrichia viscose (Asteraceae). Environ. Expt. Bot. 56:293-300. Stewart, J. and J. Button. 1975. Tissue culture studies in paphiopedilum. Amer. Orchid Soc. Bull. 44:591-599. Murphy, A. 2002.Auxin: the growth hormone. p423-451. In: L.Taiz and E. Zeiger(ed). Plant physiology(3rd). In Sinauer Associates, Inc., MA, U.S.A.. Tanaka, M., M. Tsutsui, E. Ohno, T.S. Zhou, and T. Takamura. 1995. Micropropagation of Paphiopedilum. 1. Plantlet formation in cultured transverse thin sections from seedlings grown in virto. J. Jpn. Soc. Hort. Sci. 64(Suppl. 2):596-597. Tanaka, M., E. Ohno, T. Takamura, and T.S. Zhou. 1996. Micropropagation of Paphiopedilum. 2. Induction of plantlets as the TTS sources from cultured terminal buds of flower stalks. J. Jpn. Soc. Hort. Sci. 65 (Suppl. 2):630-631. Warrag, E. I., M. S. Lesney, and D. L. Rockwood. 1990. Microprogation of field tested superior Eucalyptus grandis hybrid. New Forests 4:67-79. Welch, R. M. 1995. Micronutrient nutrition of plants. Cri. Rev. Plant Sci. 14:49-82. Yasugi, S. and N. Yagi. 1995. Mericlonal plantlet formation in Paphiopedilum by shoot tip culture. 1. Some effecting factors on shoot and root formation. J. Jpn. Soc. Hort. Sci. 64(Suppl. 1):516-517. Zaghmout, O. M. F. and W. A. Torello. 1988. Enhanced regeneration in long-term callus cultures of red fescue pretreatment with activated charcoal. HortScience 23:615-616. http://kmweb.coa.gov.tw/subject/mp.asp?mp=129 (2009/5/1)
摘要: 
本試驗以芭菲爾鞋蘭雜交種(Paph. delenatii × Paph. armeniacum)已開過花之分植株營養系苗株作為試驗材料,成功地培養去葉植株之短縮莖,並從腋芽發育小苗。試驗中將培養材料在滅菌前先預處理乾燥2週,可提高成功率。而以含0.1% Tween 20之1% 次氯酸鈉溶液震盪滅菌9分鐘成活率較高。培養材料以選擇帶葉片5片者,成活率較高。另外培養基無生長調節劑者,初代培養之成功率可提高至66.7%。
MS培養基中CuSO4 .5H2O濃度提高至0.75 mg/l,可長出1個芽。在液體培養基中培養新增芽體數較在固體培養基中多,並可以減少根之形成。MS配方中H3BO3濃度降至原來的2/3,可促使芽體提早形成。
當MS培養基減去硝酸銨與硝酸鉀時,添加酵母萃取物取代氮源能新增3.67個芽體。雖然在高濃度(8或16 mg/l)的TDZ較低濃度者可促進芭菲爾鞋蘭芽體增生,但仍比添加BA 50 mg/l之效果低。此外,除葉處理可增加芽體之形成並抑制培植體長根。

The clonal plants of blossomed Paphiopedilum (Paph. delenatii × Paph. armeniacum) hybrids propagated by division were used as materials for initial culture. It succeeded to develop lateral shoots from the stem explants.When the material drought for two weeks before culture, the survival rate increased. To sterile by 10% NaOCl with 0.1% Tween 20 for 9 minutes resulted in higher survival rate. Also, material with five leaves resulted in higher survival rate. Moreover, explants cultured on hormone free medium enhanced the survival rate to 66.7%.
When the concentration of CuSO4 .5H2O in MS medium raised to 0.75 mg/l, explant sprouted 1 shoot. New shoots developed more shoots, but shorter roots from explants in liquid medium than that in solid medium. In the case of decreasing 1/3 the boron concentration of MS medium promoted shoots earlier.
When yeast extract as nitrogen source in the MS medium without NH4NO3 and KNO3, proliferated 3.67 shoots. Although explants on the medium containing higher concentration of TDZ (16 mg/l) proliferated more shoots than less one (8 mg/l ). The proliferated shoots were fewer than on medium containing BA 50 mg/l. In addition, explants excised leaves enhance new shoots production and inhibited rooting.
URI: http://hdl.handle.net/11455/29050
其他識別: U0005-0408200918330800
Appears in Collections:園藝學系

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.