Please use this identifier to cite or link to this item:
標題: 利用疊氮化鈉培養基誘變長壽花
The Mutation of Kalanchoe via Sodium Azide in vitro
作者: 蘇姫泰
Tejakhod, Sujittra
關鍵字: Shoot regeneration;芽體再生;Flower bud explant;Leaf explant;Mutant;花芽培植體;葉培植體;誘變
出版社: 園藝學系所
引用: Aida, R. and M. Shibata. 1996. Transformation of Kalanchoe blossfeldiana mediated by Agrobacterium tumefaciens and transgene silencing. Plant Science 121: 175- 185. Ahmed, E.U., T. Hayashi and S. Yazawa. 2004. Auxins increase the occurrence of leaf- colour variants in Caladium regenerated from leaf explants. Scientia Hort. 100:153-159. Avan, M.A., C.F. Konzak, J.N. Rutger and R.A. Nilan. 1980. Mutagenic effects of sodium azide in rice. Crop Sci 20: 633(Abstract). Bailey, L.H. 1949. Manual of Cultivated Plants: Most commonly grown in the continental United States and Canada. Macmillan Publishing Company, New York, USA. P. 466-467. Bhagwat, B. and E.J. Duncan. 1998. Mutation breeding of banana cv. Highgate (Musa spp., AAA Group) for tolerance to Fusarium oxysporum f.sp. cubense using chemical mutagens. Scientia Hort. 73:11-22. Bhate, R.H. 2001. Chemically induced floral morphological mutations in two cultivars of Ipomoea purpurea (L.) Roth. Scientia Hort. 88: 133-145. Bhat, T.A., M. Sharma and M. Anis. 2007. Comparative analysis of mitotic aberrations induced by diethyl sulphate (DES) and sodium azide (SA) in Vicia faba L. (Fabaceae). Pak J Biol Sci 10:783-787. Broertjes, C., and L. Leffring. 1972. Mutation breeding of Kalancho&euml;. Euphytica 21:415-423. Brulfert, J., J. Vidal, P. Gadal and O. Queiroz. 1982. Daily rhythm of phosphoenolpyruvate carboxylase in Crassulacean acid metabolism plants. Planta 156:2(Abstract). C&eacute;sar, E.G. and P. R. Morales. 1997. Sodium azide induces mitotic recombination in Drosophila melanogaster larvae. Mutation Research 389:157–165. Chang, H.C. and C. Y. Chu. 2005. Gerbera mutation breeding via floral culture with SA.植物種苗 Seed & Nursery (Taiwan) 7:23-33. Chen, C., G.L. Wang, L.M. Tian and R.S. Cui. 2004. Embryoid induction and regeneration in callus of Kalanchoe blossfeldiana. Acta Horticulturae Sinica 31:249-252. Chu, C. Y. 2004.Application of biotech on the breeding of poinsettia. Project report of 93 AS-4.2.1.T-a1(2), Agricultural Council, R.O.C. Chu, C. Y., Chiang, C. Y. , Huang, T. H. and Chang, H. C. 2006. Application of plant tissue culture on ornamentals’ breeding. (2006/3/8) Japan/Taiwan Symposium, Application of Biotechnology to Horticulture Industry. Saba University, Japan. Christensen,B. and R. M&uuml;ller. 2008. Kalanchoe blossfeldiana transformed with rol genes exhibits improved postharvest performance and increased ethylene tolerance. Postharvest Biology and Technology. doi:10.1016/j.postharvbio.2008. 08.010. Crispi, M.L., S.E. Ullrich and R.A. Nilan. 1987. Investigation of partial sterility in advanced generation, sodium azide-induced lines of spring barley. Theor Appl Genet 74:402-408. Dickens, C.W.S. and J. van Staden. 1988. The in vitro flowering of Kalanch&ouml;e blossfeldiana Poellniz. Journal of Experimental Botany 39:61-471. Dole, J.M. and H. F. Wilkins. 1999. Floriculture : Principles and Species. Prentice Hall, NJ, U.S.A. P. 403-408. Engvild, K.C. 2005. Mutagenesis of the model grass Brachypodium distachyon with sodium azide. Ris&oslash; National Service Department., Roskilde, Denmark. Ris&oslash;-R- 1510(EN) Report. Frello, S., E. Venerus and M. Serek. 2002. Regeneration of various species of Crassulaceae, with special reference to Kalancho&euml;. J. Hortic. Sci. Biotech. 77: 204–208. Ganesan, M., P. Bhanumathi and N. Jayabalan. 2005. Mutagenic effect of sodium azide on somatic embryo regeneration and root growth of cotton (Gossypium hirsutum L. CV. SVPR2). Journal of Agricultural Technology 1:365-380. Gao, M.W., Q.H. Cai and Z.Q. Liang. 1992. In vitro culture of hybrid indica rice combined with mutagenesis. Pl. Breed., 108:104-110. Garc&ecirc;s, H. M. P., C. E. M. Champagne, B. T. Townsley, S. Park, R. Malho, M. C. Pedroso, J. J. Harada, and N. R. Sinha. 2007. Evolution of asexual reproduction in leaves of the genus Kalancho&euml;. PNAS 103:15578-15583. Gavazzi, G., C. Tonelli, G. Todesco, E. Arreghini, F. Raffaldi, F. Vecchio, G. Barbuzzi, M. G. Biasimi, and F. Sala. 1987. Somaclonal variation versus chemically induced mutagenesis in tomato (Lycopersicon esculentum L.). Theoret. Appl. Genet. 74:733-738. Gonz&aacute;lez, G., M. P&eacute;rez, I. Santana, M. Marcet, J. Staněk, J. Moravcov&aacute; and J. Jarv. 1990. Mutagenic activity of 3-azido-1,2-propanediol and sodium azide applied to sugar cane callus cells. Biologia Planarum 35: 388(Abstract). Graf, A. B. 1976. Exotica, Series 3: Pictorial Cyclopedia of Exotic Plants from Tropical and Near Tropic Regions. 9th ed. Roehrs Company Inc, NJ. P. 1637-1638. Guo, D.P., Z.J. Zhu, X.X. Hu and S.J. Zheng. 2005. Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai). Plant Cell, Tissue and Organ Culture 83:123-127. Hasegawa, H. and M. Inoue. 1980. Mutation effect of sodium azide in barley. Japan J. Breed 30: 20-26. Hern&aacute;dez, J.R., M.C. Aime and B. Newbry. 2004. Aecidium kalanchoe sp. nov., a new rust on Kalanchoe blossfeldiana (Crassulaceae). Mycological Research 108: 846-848. Hwang, S.G., M.Y. Lee, Y.H. Park, I. Sivanesan and B.R. Jeong. 2008. Suppression of stem growth in pot kalanchoe ‘Gold Strike’ by recycled subirrigational supply of plant growth retardants. African Journal of Biotechnology 7:1487–1493. Ilbas, A.I., Y. Eroglu and H.E. Eroglu. 2005. Effects of the application of different concentrations of NaN3 for different times on the morphological and cytogenetic characteristics of barley (Hordeum vulgare L.) seedlings. Journal of Integrative Plant Biology Formerly Acta Botanica Sinica 47:1101-1106. Izumikawa. Y., S. Takei, I. Nakamura and M. Mii. 2008. Production and characterization of inter-sectional hybrids between Kalanchoe spathulata and K. laxiflora ( = Bryophyllum crenatum ). Euphytica 163:123-130. Jain, S.M. 2005. Major mutation-assisted plant breeding programs supported by FAO/IAEA. Plant Cell, Tissue and Organ Culture 82:113-123. Jaiswal, S. and S. Sawhney. 2006. Correlation of epiphyllous bud differentiation with foliar senescence in crassulacean succulent Kalanchoe pinnata as revealed by thidiazuron and ethrel application. Journal of Plant Physiology 163:717-722. Jaiswal, S. and S. Sawhney. 2008. Thidiazuron-induced hypertrophic growth from foliar disks of Kalanchoe pinnata leads to visualization of a bioactive auxin gradient across the leaf plane. In Vitro Cell.Dev.Biol.-Plant 44:65–68 Johnson, E.L. 1948. Response of Kalanchoe tubiflora to x-radiation. Plant Physiology 23: 544-556. Kebenei, Z., E.C. Sisler, T. Winkelmann and M. Serek. 2003. Efficacy of new inhibitors of ethylene perception in improvement of display life of kalancho&euml; (Kalancho&euml; blossfeldiana Poelln.) flowers. Postharvest Biology and Technology 30: 169-176. Kakeni, M. 1979. Studies on the tissue culture of carnation V. Induction of redifferentiated plants from petal tissue. Bulletin of the Hiroshima Agricultural Collage 6: 159-166. Kantia, A. and S.L. Kothari. 2002. High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of Dianthus chinensis L. Scientia Hort. 96:205-212. Kawamitsu, Y., T. Tokeshi, K. Kawamoto, A. Nose and S. Murayama. 2000. On the production of photosynthesis mutant in Kalanchoe pinnata using the gamma-ray irradiation. In case of a acute irradiation. The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus,Okinawa, Japan 47:17-28. Koh, Y.C., F.T. Davies Jr. 2001. Mutagenesis and in vitro culture of Tillandsia fasciculata Swartz var. fasciculata (Bromeliaceae). Scientia Hort. 87:225-240. Kulka, R.G. 2008. Hormone control of root development on epiphyllous plantlets on Bryophyllum (Kalancho&euml;) marnierianum: role of auxin and ethylene. Journal of Experimental Botany 59:2361-2370. Levi, C. and M. Gibbs. 1975. Carbon dioxide fixation in isolated Kalanchoe chloroplasts. Plant physiol 56:164-166. Love, J.W. 1980. Kalanchoe. In: R.A., Larson (eds.) Introduction To Floriculture. Academic Press Inc., New York, U.S.A. P. 411-433. Mahna, S.K. and R. Garg. 1898. Induced mutation in Petunia nyctaginiflora Juss. Biologia plantarum 31:152-155. Merkle, S.A., W.A. Parrot and B.S. Flinn. 1995. Morphogenic aspects of somatic embryoenesis. In: T.A. Thrope (eds.) In vitro embryogenesis in plants. Kluwer academic publishers., The Netherland. P.155-203. Mensah, J.K. and B. Obadoni. 2007. Effects of sodium azide on yield parameters of groundnut (Arachis hypogaea L.) African Journal of Biotechnology 6:668-671. Miller, R., V. Kaul, J.F. Hutchinson, G. Maheswaran and D. Richards. 1991. Shoot Regeneration from Fragmented Flower Buds of Carnation (Dianthus caryophyllus).Annals of Botany 68:563-568. Mitsukuri, K., G. Mori, M. Johkan, Y. Shimada, K.-I. Mishiba, T. Morikawa and M. Oda. 2009. Effects of explant position and dark treatment on bud formation in floret culture of Ponerorchis graminifolia Rchb.f. Scientia Hort. 243-247. Mondal, S., A.M. Badigannavar, D.M. Kale and G.S.S. Murty. 2007. Induction of genetic variability in a disease-resistant groundnut breeding line. BARC Newsletter 285: 237-347. Modgil, M., R. Handa and D.R. Sharma. 1999. Directshoot regeneration from excised leaves of in vitro raised shoots of clonal apple rootstock, MM106. Scientific correspondence 76:278-279. Murashige, T., F. Skoog. 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiolol Plant 15:472-497. Muthusamy, A., K. Vasanth, D. Sivasankari, B. R. Chandrasekar and N. Jayabalan. 2007. Effects of mutagens on somatic embryogenesis and plant regeneration in groundnut. Biologia Plantarum 3: 430-435. Nakano, M., M. Nagai, S. Tanaka, M. Nakata and T. Godo. 2005. Adventitious shoot regeneration and micropropagation of the Japanese endangered Hylotelephium sieboldii (Sweet ex Hook.) H. Ohba and H. sieboldii var. ettyuense (Tomida) H. Ohba. Plant Biotechnology 22:221-224. Nakornthap, A. 1973. Radiation-induced somatic mutation in Kalanchoe (Kalanchoe laciniata). The Kasetsart Journal 7: 13-18. Neyland, M., Y.L. Ng, and K.V. Thimann. 1963. Formation of anthocyanin in leaves of Kalanchoe blossfeldiana, a photoperiodic response. Plant Physiol. 38: 447–451. Nielsen, A.H., C.E. Olsen and B.L. M&oslash;ller. 2005. Flavonoids in flowers of 16 Kalancho&euml; blossfeldiana varieties. Phytochemistry 66: 2829-2835. Olsen, O., X. Wang and D. von Diter. 1993. Sodium azide mutagenesis: Preferential generation of AT-->GC transitions in the barley Antl8 gene. Proc. Natl. Acad. Sci 90: 8043-8047. Osborn, M., Weber, K., 1980. Damaged cellular function by trifluoperazine, a calmodulin specific drug. Experimental Cell Research 130: 484–489. Owais, W.M., M.A. Zarowitz and R.A. Gunovich. 1978. A mutagenic in vitro metabolite of sodium azide. Mutation Reseach 53: 355(Abstract). Papafotiou, M., A. N. Martini. 2009. Effect of position and orientation of leaflet with respect to plant growth regulatios on micropropagation of Zamioculcas zamiifolia Engl. (ZZ). Scientia Hort. 120: 115-120. Pertuit, A.J. 1997. Kalanchoe. In: Gaston, M.L., S. A. Carver, C.A. Irwin, and A. Larson (eds.) Tips on growing specialty potted crops. Ohio Florists'' Association, Columbus, Ohio, USA. P.94-101. Prina, A.R. and E.A. Favret. 1983. Parabolic effect in sodium azide mutagenesis in barley. Hereditas 98:89-94(Abstract). Ragunathan, I., and N. Panneerselvam. 2007. Antimutagenic potential of curcumin on chromosomal aberrations in Allium cepa. Journal of Zhejiang University SCIENCE B 8:470-475. Rank, J. and M. H. Nielsen. 1997. Allium cepa anaphase–telophase root tip chromosome aberration assay on N-methyl-N-nitrosourea, maleic hydrazide, sodium azide, and ethyl methanesulfonate. Mutation Research 390: 121-127. Rascio, A., M. Russo, L. Mazzucco, C. Platani, G. Nicastro and N. D. Fonzo. Enhanced osmotolerance of a wheat mutant selected for potassium accumulation. 2001. Plant Science 160: 441-448. Roy, S. and A.K. Biswas. 2005. Isolation of a white flowered mutant through seed culture in Spathoglottis plicata Blume. Cytologia 70: 1-6. Sander, C., R.A. Nilan, A. Kleinhofs and B.K. Vig. 1978. Mutagenic and chromosome- breaking effects of azide in barley and human leukocytes. Mutation Research 50:67(Abstract). Sanikhani, M., H. Mibus, B.M. Stummann and M. Serek. 2008. Kalanchoe blossfeldiana plants expressing the Arabidopsis etr1-1 allele show reduced ethylene sensitivity. Plant Cell Rep 27: 729-737. Sanikhani, M., S. Frello and M. Serek.2006. TDZ induces shoot regeneration in various Kalancho&euml; blossfeldiana Poelln. cultivars in the absence of auxin. Plant Cell, Tissue and Organ Culture 85:75-82. Schum, A. 2003. Mutation Breeding in Oramentals: An Efficient Breeding Method?. Proc. 21st IS on Classical/Molecular Breeding. Acta Hort 612: 47-60. Schum, A. and W. Preil. 1998. Induced mutation in ornamental plants. In: Jain, S.M., D.S. Brar, B.S. Ahloowalia. (eds.) Somaclonal variation and induced mutation in crop improvement. Kluwer Academic Publishers, The Netherland. P. 322- 365. Schwabe, W. W. 1985. Kalanchoe blossfeldiana. In: Halevy, A.H. (eds.) Handbook of Flowering. Vol. 3. CRC Press, Florida, U.S.A. P. 217-235. Siddiqui, S., M.K. Meghvansi and Z. Hasan. 2007. Cytogenetic changes induced by sodium azide (NaN3) on Trigonella foenum-graecum L. seeds. South African Journal Botany 73: 632-635. Slamenova, D. and A. Gabelova. 1980. The effects of sodium azide on mammalian cells cultivated in vitro. Mutat Res. 71:253-61.(Abstract). Smith, R.H. and A.E., Nightingale. 1979. In vitro propagation of Kalancho&euml;. Hort Science 14: 20. Srivastava, A. and K. Kapoor.2008. Seed yield is not impaired by chromosome stickiness in sodium azide treated Trigonella foenum-graecum. Cytologia 73:115–121. Szarejko, I. and B.P. Forster. 2007. Doubled haploidy and induced mutation. Euphytica 158:359-370. Tiwari, V., B.D. Singh and K.N. Tiwari. 1998. Shoot regeneration and somatic embryogenesis from different explants of Brahmi [Bacopa monniera (L.) Wettst.]. Plant Cell Reports 17:538-543. Thomas, M. and S.L. Ranson. (1954) Physiological Studies on Acid Metabolism in Green Plants. III. Further Evidence of CO2-Fixation During Dark Acidification of Plants Showing Crassulacean Acid Metabolism. New Phytologist 53: 1(Abstract). Tomsone, S., D. Gertnere and D. Novikova. 2004. The influence of thidiazuron on shoot regeneration and proliferation of rhododendrons in vitro. Acta Universitatis Latviensis, Biology 676:239–242. Topoonyanont, N., R. Ampawan, and P. C. Debergh. 2000. Reversion of Limonium hybrid `Misty Blue'' inflorescence development and its applicability in micropropagation. Scientia Hort. 83:283-299. Tunjit, P. and C.Y. Chu. 2008. Micropropagation of Euphorbia milii Desmoul via cyathium explants. Ms Diss., Dept. of Hort., The National Chung Hsing Univ. Taichung, Taiwan. T&uuml;rkan, A.D., K.M. Khawar, C.Y. &Ccedil;iftҫi and S. &Ouml;zcan. 2006. Effects of Mutagenic Sodium Azide (NaN3) on In Vitro Development of Four Pea (Pisum sativum L.) cultivars. International Journal of Agriculture & Biology 8:349-351. Vendrame, W.A., I. Maguire and V.S. Carvalho. 2007. In vitro propagation and plantlet regeneration from Doritaenopsis purple gem ‘Ching Hua’ flower explants, Hort. Sci 42:1256–1258. Van Harten, A.M. 1998. Mutation Breeding: Theory and Practical Applications. Cambridge University Press, United Kingdom. 353pp. Van Voorst, A. and J.C. Arends. 1982. The origin and chromosome numbers of cultivars of Kalanchoe blossfeldiana Von Poelln.: their history and evolution. Euphytica 31:573-584. Varga, A., L.H. Thoma and J. Bruinsma.1988. Effects of auxins and cytokinins on epigenetic instability callus-propagated Kalanchoe blossfeldiana Poelln. Plant Cell, Tissue and Organ culture 15:223(Abstract). Walther, F. and A. Sauer. 1986. In vitro mutagenesis in roses. Acta Hort. 189:37-46. Wetherall, D.F. and D.K. Dougall. 1976. Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue. Physiol. Plant. 37: 97- 103 Wu, D.L., S.W. Hou, P.P. Qian , L.D. Sun, Y.C. Zhang and W.J. Li. 2009. Flower color chimera and abnormal leaf mutants induced by 12C6+ heavy ions in Salvia splendens Ker-Gawl. Scientia Hort. doi:10.1016/j.scienta.2009.02.022. Xu, L.F., F.W. Ma and D. Liang. 2009. Plant regeneration from in vitro cultured leaves of Lanzhou lily (Lilium davidii var. unicolor). Scientia Hort 119:459-461. Yang, L.J., M. Hidaka, H. Masaki and T. Uozumi. 1995. In vitro plant regeneration from leaf and petiole explants of Hibiscus syriacus L. Plant Tissue Culture Letters 12:173-177. Zamora, A.B., S.S. Gruezo and S.V. Siar. 1998. Somaclonal variation: breeder’s tool for recovering potential cultivars in ornamentals (Kalanchoe). Annual Scientific Conference of the Federation of Crop Science Societies of the Philippines 23:71. Zhang, L., Li, L., Ban, L., An, W., Liu, S., Li, X., Xue, B., Xu, Y., 2000. Effect of sodium azide on mitochondrial membrane potential in SH-SY5Y human neuroblastoma cells. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 22, 436–439. CBI: Centre for the promotion of imports from developing countries, CBI market survey: the plant material market in the Netherland. 24 February 2009. ( nthenetherlands) FAO/IAEA Mutation Variety Database. 12 February 2009. < http://www- /default.htm>. Kalanchoe Blossfeldiana Kalanchoe. The Environmental Horticulture Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. 12 February 2009. < FP309>. Taichung City General Information, Taichung City’s Flower. 24 February 2009.(
本試驗以疊氮化鈉為誘變劑對長壽花‘燈塔’和 ‘日出’進行誘變。以兩品種的二級小花蕾為培植體,培養在½ MS鹽類濃度、 BA 1mgl-1 和NAA 0.5mgl-1的培養基中有較佳的芽體增殖率。以‘燈塔’的葉緣為培植體培養在含疊氮化鈉之½ MS鹽類濃度、BA 1 mgl-1 和NAA 0.5 mgl-1培養基時有較佳的增殖率;以‘日出’的葉中肋為培植體,培養在含疊氮化鈉之½ MS鹽類濃度、2 mgl-1 BA 和 NAA 0.5 mgl-1培養基時,有較佳的增殖率。
以疊氮化鈉處理後,兩品種的組織培養均有較低的存活率。在誘變試驗中,以‘燈塔’花蕾或葉為培植體之誘變率分別為77.8 或 53.1%;而以‘日出’花蕾或葉為培植體在誘變試驗中之誘變率分別為53.1%或 41%。

The application of induced mutation in vitro by using sodium azide (SA) consisted of culturing explant in SA medium, and soaking explant in mutagen solution treatment. Floret bud from 2nd level of Kalanchoë blossfeldiana ‘Beacon' and ‘Sunrise cyme inflorescence were used as explant that culture media was used ½ MS containing BA 1mgl-1 combined NAA 0.5mgl-1 as presented as the most proliferated shoots medium in pretreatment. Whereas, in leaf experiment were used marginal leaf explant of ‘Beacon' cultured on basal medium consisted of BA 1 mgl-1 combined 0.5 mgl-1 NAA, and leaf vein explant of ‘Sunrise' cultured in media supplemented with 2 mgl-1 BA and 0.5 mgl-1 NAA, combined with SA treatment to induced mutation.
Plantlets from control groups of ‘Beacon' and ‘Sunrise' were found up to 90% of survival, whereas both of application of culturing explants in SA medium or soaking in this mutagen solution had lower shoot regeneration frequency. The mutation rate via flower buds or leaf explant of ‘Beacon' was 77.8 or 53.1%, respectively; whereas the rate of mutation cv. Sunrise was 53.1% from flower buds as well as 41% from leaf explants.
The results of this experiment proved that SA regenerated plants showed greater variation in the leaf phenotype compared to the plants regenerated from the control. Furthermore, mutants of floral change were accompanied with leaf change. The original of ‘Beacon' cultivar had flower color as bright orange, but mutant plants were found ‘lighter orange' and ‘yellow'. In addition, the petal colour of ‘Sunrise' mutant changed from bright red into ‘deep red', ‘dull red', or ‘orange'. Early flowering was one of induced mutation in physiological trait that obtained from both of ‘Beacon' and ‘Sunrise' mutants.
其他識別: U0005-1107200916023400
Appears in Collections:園藝學系

Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.