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標題: 利用疊氮化鈉培養基誘變長壽花
The Mutation of Kalanchoe via Sodium Azide in vitro
作者: 蘇姫泰
Tejakhod, Sujittra
關鍵字: Shoot regeneration;芽體再生;Flower bud explant;Leaf explant;Mutant;花芽培植體;葉培植體;誘變
出版社: 園藝學系所
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本試驗以疊氮化鈉為誘變劑對長壽花‘燈塔’和 ‘日出’進行誘變。以兩品種的二級小花蕾為培植體,培養在½ MS鹽類濃度、 BA 1mgl-1 和NAA 0.5mgl-1的培養基中有較佳的芽體增殖率。以‘燈塔’的葉緣為培植體培養在含疊氮化鈉之½ MS鹽類濃度、BA 1 mgl-1 和NAA 0.5 mgl-1培養基時有較佳的增殖率;以‘日出’的葉中肋為培植體,培養在含疊氮化鈉之½ MS鹽類濃度、2 mgl-1 BA 和 NAA 0.5 mgl-1培養基時,有較佳的增殖率。
以疊氮化鈉處理後,兩品種的組織培養均有較低的存活率。在誘變試驗中,以‘燈塔’花蕾或葉為培植體之誘變率分別為77.8 或 53.1%;而以‘日出’花蕾或葉為培植體在誘變試驗中之誘變率分別為53.1%或 41%。

The application of induced mutation in vitro by using sodium azide (SA) consisted of culturing explant in SA medium, and soaking explant in mutagen solution treatment. Floret bud from 2nd level of Kalanchoë blossfeldiana ‘Beacon' and ‘Sunrise cyme inflorescence were used as explant that culture media was used ½ MS containing BA 1mgl-1 combined NAA 0.5mgl-1 as presented as the most proliferated shoots medium in pretreatment. Whereas, in leaf experiment were used marginal leaf explant of ‘Beacon' cultured on basal medium consisted of BA 1 mgl-1 combined 0.5 mgl-1 NAA, and leaf vein explant of ‘Sunrise' cultured in media supplemented with 2 mgl-1 BA and 0.5 mgl-1 NAA, combined with SA treatment to induced mutation.
Plantlets from control groups of ‘Beacon' and ‘Sunrise' were found up to 90% of survival, whereas both of application of culturing explants in SA medium or soaking in this mutagen solution had lower shoot regeneration frequency. The mutation rate via flower buds or leaf explant of ‘Beacon' was 77.8 or 53.1%, respectively; whereas the rate of mutation cv. Sunrise was 53.1% from flower buds as well as 41% from leaf explants.
The results of this experiment proved that SA regenerated plants showed greater variation in the leaf phenotype compared to the plants regenerated from the control. Furthermore, mutants of floral change were accompanied with leaf change. The original of ‘Beacon' cultivar had flower color as bright orange, but mutant plants were found ‘lighter orange' and ‘yellow'. In addition, the petal colour of ‘Sunrise' mutant changed from bright red into ‘deep red', ‘dull red', or ‘orange'. Early flowering was one of induced mutation in physiological trait that obtained from both of ‘Beacon' and ‘Sunrise' mutants.
其他識別: U0005-1107200916023400
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