Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29072
標題: 梨莖頂培養品種間差異之研究
Studies on difference of shoot tips cultured in vitro in pear cultivars
作者: 朱秀雯
Chu, Hsiu-Wen
關鍵字: pear;梨;shoot tip culture;double phase culture;莖頂培養;雙相培養
出版社: 園藝學系
摘要: 
本研究利用組織培養技術,採用 6種華北梨、25種砂梨、2種西洋梨、4種
台灣在來梨及雜交梨之莖頂加以培養,觀察各品種生長點之形態並探討各
種群最適當的培養方法,期以建立大量迅速之繁殖方法。由不同種間莖頂
觀察之結果顯示,華北梨、中國砂梨、西洋梨及台灣梨之生長點較小,鳥
梨 A及雜交梨之太平洋則較大;不同種類及品種間之絨毛量無法明顯歸納
出特定的區別。 1/2MS添加BA 1 mg/l、kinetin 0.5 mg/l、IBA 0.2 mg/
l及adenine sulfate 4 mg/l之液態濾紙橋培養基行初代培養時,中國砂
梨、台灣在來梨及雜交梨之太平洋的莖頂培養30日後,可獲得較高的成活
率。在平均大小方面,以華北梨、西洋梨及雜交梨較小,而平均鮮重則以
華北梨較低,其他各種內之品種間差異頗大。而不同品種間生長點大小及
絨毛量對培植體之生長量並無明顯的影響。在芽體增殖培養方面,台灣梨
為增殖率最高者,鳥梨A、鳥梨B、鳥梨 C及雜交梨之太平洋次之,砂梨再
次之,西洋梨為最差者;而枝梢長度在不同種類間則無一明顯之趨勢。各
品種間生長點大小及絨毛量與芽體增殖並無明確的關係存在。當芽體增殖
率低時,華北梨及日本砂梨部份品種以雙相培養,中國砂梨以kinetin
0-0.1 mg/l及日本砂梨之廿世紀、幸水、新星等品種以kinetin 1-2 mg/l
添加於培養基中,均可提高芽體之增殖率。對於枝梢不易伸長之品種,可
添加 GA3 0.5-2 mg/l於培養基即可增加枝梢長度。在發根培養時,枝梢
基部以IBA 200 ppm溶液浸漬24小時處理再扦插於不含 auxin之1/2 MS培
養基內可獲得較高的發根率;發根不良時,可將枝梢扦插於1/2MS內添加
NAA 2 mg/l之培養基中,經暗處理6日後再移入不含auxin之 1/2MS培養基
內則可提高發根率。一般而言,台灣在來梨及雜交梨之太平洋發根情形較
佳,中國砂梨次之,而日本砂梨則屬於較難發根的種類。

This study is conducted to investigate the morphology of
the apical meristems of six sinensis pears, twenty-five sand
pears, two pears, four Taiwan native pears and one hybrid pear.
The observation showed that the apical meristems of sinensis
pears, China sand pears, pears and evergreen pear were smaller,
NiauliA and hibrid pear were bigger. The higher survival
percentage were achieved when shoot tip explants of China sand
pears, Taiwan native pears and hybrid pear which cultured on
the paper bridge medium with 1/2MS containing 1 mg/l BA, 0.5 mg/
l kinetin, 0.2 mg/l IBA and 4 mg/l adenine sulfate for 30 days.
A smaller average size of bud were observed in sinensis pears,
pears and hybrid pears than other varieties. Additionally,
shoot tip explants was found to be the lowest in the average
fresh weight in sinensis pears. In the case of shoot
proliferation, the multiplication rate of the evergreen pear
was the highest among all, followed by NiauliA, NiauliB,
NiauliC, hybrid pear, sand pears, and pears is the lowest.
Sinensis and some Japanese sand pears could increase the shoot
multiplication by using double-phase medium culture. Axillary
shoots proliferation was increased when China sand pear
explants were cultured on MS with 0-0.1 mg/l of kinetin,
although 1-2 mg/l kinetin favored multiplication of explants
for Japanese sand pears. Minimal shoot elongation in vitro of
pear varieties, which cultured on MS with 0.5-2 mg/l GA were
obtained enchancment of shoot elongation. In rooting culture,
the bases of the microshoot were dipped in 200 ppm IBA solution
by 24 hours resulted in better rooting rate. In the case of
poor rooting, shoots were placed on a 1/2MS medium with 2 mg/l
NAA and incubated in darkness for 6 days, the propagules
transplanted to an auxin-free 1/2MS medium increased rooting
rate after 24 days.
URI: http://hdl.handle.net/11455/29072
Appears in Collections:園藝學系

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