Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29104
標題: 玉花四季蘭無菌播種與根莖繁殖
Seed Germination and Rhizome Propagation of Cymbidium ensifolium 'Yuh Hwa' in vitro
作者: 林淑婷
Lin, Shu-Ting
關鍵字: Cymbidium ensifolium (L.) Sw;四季蘭;seed germination;integument;種子發芽;珠被
出版社: 園藝學系所
引用: 王博仁. 1981. 蕙蘭屬的無菌播種與器官分化. 中央研究院植物研究所專刊第四號: 22-30. 王博仁、邱金春. 1984. 蘭的繁殖法. 台灣省農業試驗所特刊. 14: 73-108. 利幸貞. 1992. 一、素心蘭與四季蘭之無菌播種. 二、溫度對四季蘭開花之研究. 國立台灣大學園藝研究所碩士論文. 台北. 呂依倫、李志仁、李哖. 1992. 培養基成分對素心蘭種子無菌發芽之影響.中國園藝 38: 161-169. 呂依倫、李哖. 1990. 鳳蘭之無菌發芽.中國園藝 36: 198-209. 呂依倫. 1988a. 素心蘭與鳳蘭之無菌播種與器官分化. 國立台灣大學園藝研究所碩士論文. 台北. 呂依倫. 1988b. 素心蘭種子發芽及器官分化. 中國園藝 33: 336. 李志仁、李哖. 1991. 報歲蘭和素心蘭種子無菌發芽. 中國園藝 37: 289. 李志仁. 1991. 報歲蘭與素心蘭之開花與種子無菌發芽之研究. 國立台灣大學園藝研究所碩士論文. 台北. 李勇毅. 1998. 原生托鞋蘭胚發育與無菌發芽之研究. 國立台灣大學園藝研究所碩士論文. 台北. 李勇毅. 1999. 果莢成熟度、培養基組成份與液體培養對芭菲爾鞋蘭原生種種子發芽之影響. 台灣拖鞋蘭專集Ⅱ: 10-12. 中華民國台灣省拖鞋蘭協會. 台北. 李勇毅. 2001. 果莢成熟度、培養基組成份與液體培養對芭菲爾鞋蘭屬之Paphiopedium primulinum種子發芽之影響. 中國園藝 47: 147-156. 李哖、陳美惠. 1997. 拖鞋蘭之果莢成熟度和種子前處理對無菌發芽之影響.園藝種苗科技術研究成果發表會專集 630-644. 李哖. 1990. 蘭之胚培養. 中國園藝 36: 223-244. 金花、樸炫春、廉美蘭、潘虹虹. 2007a. 玉花蘭組培根狀莖的增殖因素研究. 林業應用技術 4: 8-9. 金花、樸炫春、廉美蘭、楊金鳳、樸美英. 2007b. 玉花蘭綠芽分化及生根的組織培養條件研究. 安徽農業科學 35: 376-377, 379. 林讚標. 1988. 蕙蘭屬. 台灣蘭科植物Ⅱ: 101-108. 南天書局. 台北. 邱金春、王博仁. 1985. 嘉德麗雅蘭與蕙蘭種子發芽與芽的形成. 中國園藝 31: 10-22. 易美秀、王才義、蔡宛育. 2005. 文心蘭蒴果和胚的成熟度對種子發育的影響. 台中區農業改良場研究彙報 86: 37-45. 凃美智、李哖. 1987. 蝴蝶蘭授粉適期與果莢成熟度對種子發芽之影響. 中國園藝 33: 190-200. 徐權君. 2008. 黃道報歲蘭之授粉、種子發育與發芽以及報歲蘭之品種特性比較. 國立中興大學園藝研究所碩士論文. 台中. 莊錦華. 1983. 台灣一葉蘭無菌播種發芽與幼苗生長之研究. 國立台灣大學園藝研究所碩士論文. 台北. 莊錦華、李哖. 1983.台灣一葉蘭無菌發芽與幼苗生長.中國園藝 29: 314. 張耀乾. 1989. 台灣一葉蘭之組織培養與報歲蘭根莖之器官分化. 國立台灣大學園藝研究所碩士論文. 台北. 張莉欣. 1994. 培養基成分對素心蘭根莖生長與分化及光度對幼苗生長之影響. 國立台灣大學植物研究所碩士論文. 台北. 張正、陳盈君、嚴新富. 2005. 金稜邊蘭種子發芽形態研究. 植物種苗 7: 47-55. 陳任芳. 1992. 蘭花病毒害診斷與預防. 花蓮區農業推廣簡訊 9: 18-19. 陳任芳. 1994. 國蘭病蟲害種類消長調查及其防治方法研究. 花蓮區農業改良場研究彙報 10: 113-123. 郭珮琪. 2006. 蕙蘭產業現況與發展. 蕙蘭栽培管理手冊: 4-11. 行政院農業委員會動植物防疫檢疫局. 台北. 黃秀華. 1994. 國蘭病害之簡介. 台中區農推專訊 138期. 黃瑞啟. 2005. 蕙蘭產銷概況. p. 2-10. 蕙蘭病蟲害防治講習會-中華盆花發展協會. 南投. 楊昭容. 1982. 玉華蘭根莖分化成芽體的形態變化過程的研究. 國立台灣大學植物研究所碩士論文. 台北. 葉淑如. 1990. 黃根節蘭、繡邊根節蘭及白鶴蘭植株之生長習性與種子發芽生理. 國立台灣大學園藝研究所碩士論文. 台北. 廖敏君. 1992. 素心蘭與報歲蘭根莖器官分化與芽生長之研究. 國立台灣大學園藝研究所碩士論文. 台北. 廖曼利. 1995. 壹、光度及肥料濃度對報歲蘭營養生長與生殖生長的影響. 貳、培養基成分、光線及溫度對報歲蘭根莖生長與分化的影響. 國立台灣大學園藝研究所碩士論文. 台北. 廖曼利、張利欣、李哖. 1997. 素心蘭與報歲蘭根莖之器官分化與大量繁殖. 園藝種苗科技術研究成果發表會專集 593-629. 蔡淑華. 1975. 植物組織切片技術綱要. 茂昌圖書有限公司. 台北. 劉黃碧圓. 2000. 滲調處理對巴菲爾鞋蘭(Phiopedilum delenatii)種子發芽及幼苗生育之影響.嘉義大學學報71: 1-13. 潘貞嫈. 2007. 培養條件對報歲蘭根莖分化之影響及培植期間瓶內氣體之變化. 國立台灣大學園藝研究所碩士論文. 台北. 蕭揚區. 1978. 觀音素心蘭子房發育與種子形成之研究. 國立台灣大學植物研究所碩士論文. 台北. Arditti, J. and A. K. A. Ghani. 2000. Tansley review No. 110-Numerical and physical properties of orchid seeds and their biological implications. New Phytol. 145: 367-421. Bewley, J. D. 1997. Seed germination and dormancy. Plant Cell 9: 1055-1066. Buyun, L., A. Lavrentyeva, L. Kovalska, and R. Ivannikov. 2004. In vitro germination of seeds of some rare tropical orchids. Acta Universitatis Latviensis, Biology. 676: 159-162. Chang, C. and W. C. Chang. 1998. Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Rep. 17: 251-255. Chang, C. and W. C. Chang. 2000a. Effect of thidiazuron on bud development of Cymbidium sinense Willd in vitro. Plant Growth Regul. 30: 171-175. Chang, C. and W. C. Chang. 2000b. Micropropagation of Cymbidium ensifolium var. misericors through callus-derived rhizomes. In Vitro Cell. Dev. Biol. - plant 36:517-520. Chang, C., Y. C. Chen, and H. F. Yen. 2005. Protocorm or rhizome? The morphology of seed germination in Cymbidium dayanum Reichb. Bot. Bull. Acad. Sin. 46: 71-74. Chu, C. and K. W. Mudge. 1994. Effects of pre-chilling and liquid suspension culture on seed germination of the yellow Lady''s slippers orchid (Cypripedium calceolus var. puvescens). Lindleyana 9: 153 -159. Chung, S. K., M. Y. Chung, C. K. Kim, K. B. Lim, and J. D. Chumg. 2006. Effect of culture media on rhizome formation and subsequent growth from shoot tip culture of Cymbidium goeringii. Flower Res. J. 14: 289-295. Chung, S. K., M. Y. Chung, C. K. Kim, K. B. Lim, and J. D. Chung. 2007. Effect of medium composition on multiple shooting and subsequent growth from rhizome via shoot tip culture of Cymbidium goeringii. Flower Res. J. 15: 111-116. Copeland, C. D. and M. B. Mcdonal. 1995. Seed science and technology. Cnampman and Han. New York. De Pauw, M. A., W. R. Remphrey, and C. E. Palmer. 1995. The cytokinin preference for in vitro germination and protocorm growth of Cypripedium candidum. Ann. Bot. 75: 267-275. Debeaujon, I. and M. Koornneef. 2000. Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid. Plant Physiol. 122: 415-424. Devlin, P. F., P. R. H. Robson, S. R. Patel, L. Goosey, R. A. Sharrock, and G. C. Whitelam. 1999. Phytochrome D acts in the shade-avoidance syndrome in Arabidopsis by controlling elongation growth and flowering time. Plant Physiol. 119: 909-915. Druart, P. H. and O. De Wulf. 1992. Activated charcoal catalyses sucrose hydrolysis during autoclaving. Plant Cell Tissue Organ. Cult. 32: 97-99. Du Puy, D. and P. Cribb. 2007a. The Cymbidium seed. pp.4-8. In The genus Cymbidium. Timber Press, Oregon. Du Puy, D. and P. Cribb. 2007b. The classification of Cymbidium. pp.67-330. In The genus Cymbidium. Timber Press, Oregon. Fujii, K., M. Kawano, and S. Kako. 1999. Effects of benzyladenine and α-naphthaleneacetic acid on the formation of protocorm-like bodies (PLBs) from explants of outer tissue of Cymbidium PLBs cultures in vitro. J. Jpn. Soc. Hortic. Sci. 68: 35-40. Fukai, S., A. Hasegawa, and M. Goi. 2000. Morphological characteristics and shoot regeneration potential of the fasciated rhizome of Cymbidium kanran Makino. Plant Biotechnol. 17: 259- 262. Hasegawa, A. and M. Goi. 1987. Rhizome formation in Cymbidium goeringii Reichenbach fil, and Cymbidium kanran Makino in shoot-tip cultures. J. Jpn. Soc. Hortic. Sci. 56: 70-78. Hasegawa, A., H. Ohashi, and M. Goi. 1985. Effects of BA, rhizome length, mechanical treatment and liquid shaking culture on the shoot formation from rhizome in Cymbidium faberi Rolfe. Acta Hortic. 166: 25-40. Heydecker and Coolbear. 1977. Seed treatments for improved performance-survey and attempted prognosis. Seed Sci. Technol. 5: 353-425. Hsieh, C. F., C. F. Shen, and K. C. Yang. 1994. Introduction to the flora of Taiwan: floristics, phytogeography, and vegetation. In: Huang, T. C. (ed.). Flora of Taiwan. volI 2nd Ed. Huan, L. V. T., and M. Tanaka. 2004. Callus induction from protocorm-like body segments and plant regeneration in Cymbidium (Orchidaceae). J. Hortic. Sci. Biotechnol. 79: 406-410. Huan, L. V. T., T. Takamura, M. Tanaka. 2004. Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid. Plant Sci. 166: 1443-1449. Huang, C. L. and H. Okubo. 2005a. In vitro morphogenesis from rhizomes of Cymbidium sinense. J. Fac. Agr., Kyushu Univ. 50: 11-18. Huang, C. L. and H. Okubo. 2005b. Effect of carbon sources in in vitro morphogenesis from rhizomes of Cymbidium sinense. J. Fac. Agr., Kyushu Univ. 50: 35-40. Islam, M. O., S. Matsui, and S. Ichihashi. 1999. Effects of light quality on seed germination and seedling growth of Cattleya orchids in vitro. J. Jpn. Soc. Hortic. Sci. 68: 1132-1138. Johansen, B. and H. Rasmussen. 1992. Ex situ conservation of orchids. Opera Bot. 113: 43-48. Kano, K. 1968. Acceleration of the germination of so-called "hard to germinate" orchid seeds. Amer. Orchid Soc. Bull. 37: 690-698. Khan, A. A., J. D. Maguire, G. S. Abawi, and S. Ilyas. 1992. Matriconditioning of vegetable seeds to improve stand establishment in early field plantings. J. Amer. Soc. Hort. Sci. 117: 41-47. Kim, K. W. and S. Kako. 1982. Morphological studies on organ formation in the Cymbidium shoot apex culture in vitro. J. Jpn. Soc. Hortic. Sci. 51: 443-448. Kitsaki, C. K., S. Zygouraki, M. Ziobora, S. Kintzios. 2004. In vitro germination, protocorm formation and plantlet development of mature versus immature seeds from several Ophrys species (Orchidaceae). Plant Cell Rep. 23: 284-290. Kokubu, T. Y. Kaoeda, Y. Higashi, T. Kitano, and K. Fukamizu. 1980. Organogenesis in sterile culture of oriental Cymbidium, Cymbidium kanran Makino. Mem. Fac. Agr. Kagoshima Univ. 16: 53-64. Lakon G. 1949. The topographical tetrazolium method for determining the germination capacity of the seed. Plant Physiol. 24: 389-394. Lauzer, D., S. Renaut, M. St-Arnaud, and D. Barabe′. 2007. In vitro asymbiotic germination, protocorm development, and plantlet acclimatization of Aplectrum hyemale (Muhl. ex Wild.) Torr. (Orchidaceae). J. Torrey Bot. Soc. 134: 344-348. Lee, I. L., C. Y. Edward, N. Lee, and M. C. Chung. 2008. Embryology of Phalaenopsis amabilis var. formosa: embryo development. Bot. Stud. 49:139-146. Lee, Y. I., C. F. Lu, M. C. Chung, E.C. Yeung, and N. Lee. 2007. Developmental changes in endogenous abscisic acid concentrations and asymbiotic seed germination of a terrestrial orchid, Calanthe tricarinata Lindl. J. Amer. Soc. Hort. Sci. 132: 246-252. Lee, Y. I., E. C. Yeung, N. Lee, and M. C. Chung. 2006. Embryo development in the lady’s slipper orchid, Paphiopedilum delenatii, with emphasis on the ultrastructure of the suspensor. Ann. Bot. 98: 1311-1319. Lee, Y. I., N. Lee, E. C. Yeung, and M. C. Chung. 2005. Embryo development of Cypripedium formosanum in relation to seed germination in vitro. J. Amer. Soc. Hort. Sci. 130: 747-753.. Lu, I. L., E. Sutter, and D. Burger. 2001. Relationships between benzyladenine uptake, endogenous free IAA levels and peroxidase activities during upright shoot induction of Cymbidium ensifoilum cv. Yuh Hwa rhizomes in vitro. Plant Growth Regul. 35: 161-170. Manroque, J. P. and Y. M. Gutierrez. 2006. Asymbiotic germination of Odontoglossum gloriosum Rchb. f. (Orchidaceae) under in vitro conditions. In Vitro Cell. Dev. Biol. - Plant 42: 543–547. Mensuali-Sodi, A., M. Panizza, G. Serra, and F. Tognoni. 1993. Involvement of activated charcoal in the modulation of abiotic and biotic ethylene levels in tissue cultures. Sci. Hort. 54: 49-57. Miyoshi, K. and M. Mii. 1988. Ultrasonic treatment for enhancing seed germination of terrestrial orchid, Calanthe discolor in asymbiotic culture. Sci. Hort. 35: 127-130. Miyoshi, K. and M. Mii. 1995a. Phytohormone pre-treatment for the enhancment of seed germination and protocorm formation by the terrestrial orchid, Calanthe discolor (Orchidaceae), in asymbiotic culture. Sci. Hort. 63: 263-267. Miyoshi, K. and M. Mii. 1995b. Enhancement of seed germination and protocorm formation in Calanthe discolor (Orchidaceae) by NaOCl and polyphenol absorbent treatments. Plant Tissue culture letters 12: 267-272. Miyoshi, K. and M. Mii. 1998. Stimulatory effects of sodium and calcium hypochlorite, pre-chilling and cytokinins on the germination of Cypripedium macranthos seed in vitro. Physiol. Plant. 102: 481-486. Mohamed-Yasseen, Y., S. A. Barringer, W. E. Splittstoesser, and S. Costanza. 1994. The role of seed coats in seed viability. Bot. Rev. 60: 426-439. Morel, G. M. 1960. Producting virus-free Cymbidium. Am. Orchid Soc. Bull. 29: 495-497. Morel, G. M. 1964. Tissue culture-a new means of clonal propagation of orchids. Am. Orchid Soc. Bull. 33: 473-478. Nagashima, T. 1982a. Studies on the seed germination and embryogenesis in the Cymbidium goeringii Rchb. f. and Paphiopedium insigne var. sanderae Rchb. f. J. Jpn. Soc. Hortic. Sci. 51: 94-105. Nagashima, T. 1982b. Studies on the seed germination and embryogenesis in the Bletilla striata Rchb. f. and Calanthe discolor Lindl. J. Jpn. Soc. Hortic. Sci. 51: 82-93. Nagashima, T. 1985. Embryogenesis, and seed formation and germination in Cymbidium koran Makino. J. Jpn. Soc. Hortic. Sci. 67: 792-797. Nayak, N. R., P. K. Chand, S. P. Rath, and S. N. Patnaik. 1998. Influence of some plant growth regulators on the growth and organogenesis of Cymbidium aloifolium (L.) Sw. seed-derived rhizomes in vitro. In Vitro Cell. Dev. Biol. -Plant 34:185-188. Nayak, N. R., S. Sahoo., S. Patnaik, S. P. Path. 2002. Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. (Orchidaceae). Scientia Horticulturae 94: 107-116. Ogura, Y. and H. Okubo. 2003. In vitro shoot formation from rhizome apices of Cymbidium ensifolium and C. kanran. J. Fac. Agr., Kyushu Univ. 47: 301-306. Paek, K. Y. and E. C. Yeung. 1991. The effects of 1-naphthaleneacetic acid and N6-benzyladenine on the growth of Cymbidium forrestii rhizomes in vitro. Plant Cell Tissue Organ. Cult. 24: 65-71. Paek, K. Y. and T. Kozai. 1998. Micropropagation of temperate Cymbidium via rhizome culture. Hort Technol. 8: 175-180. Peidgeon, A. 1992. The illustrated encyclopedia of orchids. P.78-81. Timber Press, Portland. Purves, S. and G. Hadley. 1976. The physiology of symbiosis in Goodyera repens. New Phytol. 77: 689-96. Rasmussen, H. N. 1992. Seed dormancy patterns in Epipactis palustris (Orchidaceae): Requirements for germination and establishment of mycorrhiza. Physiol. Plant. 86: 161-167. Rasmussen, H. N. 1995. Terrestrial Orchids from Seed to Mycotrophic Plant. Cambridge University Press, Cambridge, GreatBritain. Reigosa, M.J., X.C. Souto, and L. Gonz′alez. 1999. Effect of phenolic compounds on the germination of six weeds species. Plant Growth Regul. 28: 83-88. Rolston, M. P. 1978. Water impermeable seed dormancy. Bot. Rec. 44: 365-395. Roy, J. and N. Banerjee. 2002. Rhizome and shoot development during in vitro propagation of Geodorum densiflorum (Lam.) Schltr. Sci. Hort. 94: 181-192. Shimasaki, K. 1995. Interactive effects between cytokinin and ethephon on shoot formation in rhizome cultures of Cymbidium kanran Makino. Plant Tissue Cult. Letters 12: 27-33. Shimasaki, K. and S. Uemoto. 1990. Micropropagation of a terrestrial Cymbidium species using rhizomes developed from seeds and pseudobulbs. Plant Cell Tissue Organ. Cult. 22: 237-244. Shimasaki, K. and S. Uemoto. 1991. Rhizome induction and plantlet regeneration of Cymbidium goeringii from flower bud cultures in vitro. Plant Cell Tissue Organ. Cult. 25: 49-52. Shimura, H. and Y. Koda. 2005. Enhanced symbiotic seed germination of Cypripedium macranthos var. rebunense following inoculation after cold treatment. Physiol. Plant. 123: 281-287. Silva, J. A. T. and M. Tanaka. 2006. Multiple regeneration pathways via thin cell kayers in hybird Cymbidium (Orchidaceae). J. Plant Growth Regul. 25: 203-210. Silva, J. A. T., N. Singh, and M. Tanaka. 2006. Priming biotic factors for optimal protocorm-like body and callus induction in hybrid Cymbidium (Orchidaceae), and assessment of cytogenetic stability in regenerated plantlets. Plant Cell Tissue Organ. Cult. 84: 135-144. Steber, C. M. amd P. McCourt. 2001. A role for brassinosteroids in germination in Arabidopsis. Amer. Soc. Plant Biol. 125: 763-769. Stewart, S. L. and M. E. Kane. 2006. Symbiotic seed germination of Habenaria macroceratitis (Orchidaceae), a rare Florida terrestrial orchid. Plant Cell Tissue Organ. Cult. 86: 159-167. Su, H. J. 2000. Orchidaceae. In Flora of Taiwan, 2nd ed. Vol. 5 pp. 820–831. Department of Botany, National Taiwan University, Taipei, Taiwan. Torikata, H., Y. Sawa, and M. Sisa. 1965. Non symbiotic germination and growth of the orchid seeds. I. Studies on the medium and additive for germination of seed in Cymbidium. J. Jpn. Soc. Hortic. Sci. 34: 63-70. Tsujita, Y. O. and H. Okubo. 2006a. Effects of low nitrogen medium on endogenous changes in ethylene, auxins, and cytokinins in in vitro shoot formation from rhizomes of Cymbidium kanran. In Vitro Cell. Dev. Biol.-Plant 42: 614-616. Tsujita, Y. O. and H. Okubo. 2006b. Promotion of in vitro shoot formation from protocorm-like bodies of a hybird between tropical and temperatate Cymbidium species. J. Jpn. Soc. Hortic. Sci. 75: 334-336. Tsujita, Y. O., A. Tatsumi, S. Hayashida, and H. Okubo. 2007. Interconversion between protocorm-like-bodies (PLBs) and rhizomes in Cymbidium. J. Fac. Agr., Kyushu Univ. 52: 325-330. Ueda, H. and H. Torikata. 1968. Organogenesis in the meristem cultures of Cymbidiums. I. Studies on the effects of growth substances added to culture media under continuous illumination. J. Jpn. Soc. Hortic. Sci. 37: 56-64. Ueda, H. and H. Torikata. 1970a. Organogenesis in the meristem cultures of cymbidiums. Ⅳ. Study on cytokinin activity in the extracts from the protocorms. J. Jpn. Soc. Hortic. Sci. 39: 202-205. Ueda, H. and H. Torikata. 1970b. Organogenesis in the meristem cultures of cymbidiums. V. Anatomical and histochemical studies on phagocytosis in the mycorrhizome of Cymbidium goeringii Rhichb. F. (C. virescens Lindl.). J. Jpn. Soc. Hortic. Sci. 39: 254-260. Van der Kinderen, G. 1987. Absisic acid in terrestrial orchid seeds: a possible impact on their germination. Lindleyana 2: 84–87. Van Waes, J. M. and P. C. Debergh. 1986a. Adaptation of the tetrazolium method for testing the seed viability, and scanning eletron microscopy study of some Western European orchids. Physiol. Plant. 66: 435-442. Van Waes, J. M. and P. C. Debergh. 1986b. In vitro germination of some Western European orchids. Physiol. Plant. 67: 253-261. Vujanovic, V., M. St-Arnaud, D. Barabe, and G. Thibeault. Viability testing of orchid seed and the promotion of colouration and germination. Ann. Bot. 86: 79-86. Wang, X. 1988. Tissue culture of Cymbidium: plant and flower induction in vitro. Lindleyana 3: 184-189. Wimber, D. E. 1963. Clonal multiplication of Cymbidium through tissue culture of the shoot meristem. Amer. Orchid Soc. Bull. 32: 105-107. Yamazaki, J. and K. Miyoshi. 2006. In vitro asymbiotic germination of immature seed and formation of protocorm by Cephalanthera falcate (Orchidaceae). Ann. Bot. 98: 1197-1206. Yeung, E. C., S. Y. Zee, and X. L. Ye. 1996. Embryology of Cymbidium sinense: embryo development. Ann. Bot. 78: 105-110.
摘要: 
玉花四季蘭(Cymbidium ensifolium ‘Yuh Hwa’)授粉後14週之少數種子具有發芽能力,以授粉後22-24週之種子的發芽率最快,此時期的胚的長寬仍在持續生長,胚發育至授粉第28週後趨於平緩,授粉24週後的發芽率隨著成熟度增加而下降,授粉36週之種子具有木質化的外種皮。成熟種子使用細胞分裂素或MS溶液進行水平震盪處理一週,以1 ppm thidiazuron、1-10 ppm 2-isopentenyl adenine或0-0.2 MS鹽類溶液處理可有較高的發芽率。使用次氯酸鈉溶液或超音波震盪處理可破壞外種皮構造,利於胚對水分及養分的吸收,進而獲得較高的發芽率,顯示木質化的外種皮構造會限制種子發芽,但與TTC所測得之活力值仍有差距。
以玉花四季蘭由莖頂培養所獲得之根莖為試驗材料,將根莖培養1/6-1/2 MS鹽類濃度的培養基,有利於根莖的增殖生長。培養基含有BA可促進根莖抽芽,添加NAA可促進根系形成,根莖培養於含有0.1 mg/l BA及5 mg/l NAA的培養基則有利於根莖增殖,在含有1 mg/l Kinetin、2 mg/l BA、0.5 mg/l NAA或1 mg/l Kinetin、1 mg/l BA、2 mg/l NAA的培養基可誘導最多的芽體形成。
URI: http://hdl.handle.net/11455/29104
其他識別: U0005-2307200916253500
Appears in Collections:園藝學系

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.