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標題: 四季蘭無菌播種及其根莖生長與分化
The Seed Germination,Rhizome Growth and Differentiation of Cymbidium ensifolium in vitro
作者: 張倍瑜
Chang, Bei-Yu
關鍵字: seed germination;種子發芽;rhizome;shoot differentiation;根莖;芽體分化
出版社: 園藝學系所
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摘要: 
授粉後220及250天的‘彩虹’四季蘭種子其發芽率較190天者佳，其發芽率分別為10.8%和10.9%。種子於20g/l蔗糖的培養基中發芽率可達到5.3%，而未添加蔗糖處理者未有種子發芽。將授粉後250天的種子進行前處理，使用超音波震盪處理可有效的提升種子的發芽率，而使用1% NaOCl及0.1 N NaOH處理則會傷害到胚，而使發芽率下降，但是處理授粉後380天之種子30分鐘可提升種子的發芽率，其發芽率分別為4.6 %和4.2%。授粉後380天的種子進行液體培養，培養4週後，有較好的發芽率10.6%，但是也有高的褐化率8.7%。
四季蘭種子萌芽後，形成原球體再形成根莖，根莖具有吸收毛且不斷分枝生長，將根莖培養於1/2MS、20g/l蔗糖的培養基中，有較多的根莖數及鮮重(1.16g)，而根莖培養在1/8 MS培養基中，其芽體形成率高於1/2 MS培養基。根莖培養於含有活性碳之培養基，有較高的芽體形成率，但是多數根莖褐化。根莖培養於0.1 mg/l NAA+ 5 mg/l BA 培養基其芽體形成率達到100%，每根莖有3.3個芽體，但NAA濃度過高時，根莖生長異常且褐化率高。當TDZ的濃度愈高，所形成的芽體數愈多。液體培養試驗中，以TDZ所形成的芽數較BA、kinetin多，但是其芽體不易生長為幼苗，而逆分化為根莖。

Cymbidium ensifolium ‘Tsai Hung’in vitro germination percentage of the seeds of 220 and 250 DAP(day after pollination)were better than those of the seeds of 190 DAP ,gerimination percentage is 10.8% and 10.9%.The seeds sowing on 20g/l sucrose medium, germiniation percentage were 5.3%,but seed germination percentage of medium without sucrose is 0%.The seed of 250 DAP pretreated with ultrasound 45 min. were effective in improving the germination percentage,but seeds pretreated with 1% NaOCl and 0.1 N NaOH may destroy the seeds and resulted in the decline of germination percentage.But seeds of 380 DAP pretreated with 1% NaOCl and 0.1 N NaOH for 30min. were improving the germination percentage,which were 4.6% and 4.2%. The seeds of 380 DAP on liquid culture with 4 weeks, germination percentage were 10.6%,but 8.7% seeds were browning.
Cymbidium ensifolium seeds germinated,then formed rhizome, elongated and branched with white absorb hair. Rhizomes cultured on 1/2 MS with 20 g/l sucrose medium,produced more rhizomes and fresh weight (1.16g) .Rhizome culture on 1/8 MS medium, shoot formation percentage were better than 1/2 MS medium. Rhizome culture on medium with activated charcoal were high shoots formation percentage,but many rhizomes were browned.Rhizome culture on 0.1 mg/l NAA+ 5 mg/l BA medium ,shoot formation percentage were 100%, produced 3.3 shoots per rhizome, but high NAA concentration were produced abnormal rhizome.Media with high TDZ concentration were produced more shoots,and floral bud formation. Liquid culture experiment,TDZ were produced more shoots than BA、kinetin ,but shoot were difficult growth into plantlet,and produced shoot redifferentiation form rhizome.
URI: http://hdl.handle.net/11455/29108
其他識別: U0005-2407200910533400
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