Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29193
標題: 東方型百合之鱗莖培育與開花特性
Bulb Cultivation and Flowering Characteristic of Oriental Lily
作者: 張文欣
Chang, Wen-Hsin
關鍵字: oriental lily;東方型百合;bulb cultivation;flowering characteristic;鱗莖培育;開花特性
出版社: 園藝學系所
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摘要: 
鹿子百合(Lilium speciosum Thunb.)因地理區隔而產生不同的變種,分布於台灣及中國大陸。利用原生於台灣、日本和中國大陸之鹿子百合鱗片於器內培養,繁殖再生的小鱗莖出瓶後移至20/15℃的人工氣候室進行栽培,記錄其生育特性。以台灣原生的豔紅鹿子百合的鱗片葉生長勢較強,適合以小苗出瓶生產高品質的小苗;中國大陸的藥百合則以鱗莖生長較好,鱗片葉少,適合以冷藏鱗莖之方式栽培優良的鱗莖球。於20/15℃人工氣候室培養後,豔紅鹿子百合的鱗片葉生長較其他兩者佳,藥百合偏重於鱗莖生長,而日本鹿子百合有鱗片葉及鱗莖。
豔紅鹿子百合鱗片經器內培養所形成之小鱗莖,經剝取其鱗片培養2個月,換置不同蔗糖濃度之MS基礎培養基培養3個月後,以30 g l-1蔗糖處理的小苗生成4.3片鱗片葉為最多,60 g l-1蔗糖處理者產生鱗莖鮮重最大,重量為0.4 g。將鱗片再生之小苗分別以小苗直接移植及鱗莖冷藏,移植出瓶。豔紅鹿子百合小苗在溫室培養7個月後,鱗莖直徑以蔗糖60和90 g l-1處理之鱗莖較大,其中經蔗糖90 g l-1處理後之抽莖率達46%。
在台灣可能因高溫或冬季低溫不足,造成豔紅鹿子百合晚開花。本試驗盆植周徑10-18 cm的鱗莖待露蕾後換到30/25℃、25/20℃、20/15℃之人工氣候室。在30/25℃栽培6週後,花蕾直徑開始快速生長,且花梗長度較長。栽培於30/25℃的花蕾平均達可開花天數為184.3 天,25/20℃的花蕾平均達可開花天數為192.7 天,20/15℃下的花蕾平均達可開花天數為203天。體外花粉培養試驗之結果,以20/15℃的花粉活力較好,在30、25和20℃有發芽,發芽率各別為4、13和30%。
為了改善東方型百合的切花品質,本試驗以東方型雜交種 ‘Medusa’和‘Marco Polo’ 百合進行花芽分化與開花之栽培。以箱植法栽培 ‘Medusa’,定植後觀察花芽分化與花器發育。定植時已有花器原體,定植後第二週外花被100 %發育,定植後第三週70 %的內花被發育及30 %的雄蕊形成,定植後第四週雌蕊發育達100 %。小花梗在第八週與第九週間,長度劇烈增加,第十週花蕾開始變大。花蕾開始轉色時即可採收,切花瓶插壽命為13天。將周徑14-16 cm 的‘Marco Polo’浸泡在不同濃度的激勃素,經不同時間處理後種植,‘Marco Polo’鱗莖以激勃素200 mg l-1和300 mg l-1 經浸泡處理10 min所生長的花蕾有伸長的趨勢。

Lilium speciosum Thunb. evolves variations due to geographic isolation, and distribution in Taiwan and China. We cultivated scales of native L. speciosum Thunb. collected from Taiwan, Japan and China in vitro for reproduction of regenerated bulblets, which were further cultivation in the phytotron (20/15°C) for records of growth characteristics. The status of scale-leaves of L. speciosum Thunb. var. gloriosoides Baker derived from Taiwan was the best and applicable to production of high quality plantlets ; the growth of L. speciosum Thunb. var. gloriosoides Baker derived from China preferring bulbs to scale-leaves was applicably cultivated to excellent bulbs via cold storage bulbs. And the plantlet with the scale-leaves and bulb of L. speciosum Thunb derived from Japan.
With scales derived from L. speciosum Thunb. var. gloriosoides Baker’s bulblets that have been cultivated in vitro, the bulblets cultivated from these scales for 2 months were transplanted to MS culture media containing sucrose with different concentrations. After three months culture, the maximum 4.3 scale-leaves were observed on one plantlet processed in the 30 g l-1 sucrose ; the bulb’s heaviest fresh weight, 0.4 g, was obtained in the 60 g l-1 sucrose. For transplantation, the plantlets reproduced from scales were transplanted through two methods such as directly transplanted plantlets and cold storage bulbs. With plantlets of L. speciosum Thunb. var. gloriosoides Baker having cultivated in the greenhouse for seven months, the diameters of bulbs processed in both 60 g l-1 and 90 g l-1 sucrose were larger than those in other sucrose and the percentage of sprouting for bulbs processed in the 90 g l-1 sucrose was the highest, 46%.
Lilium speciosum Thunb. var. gloriosoides Baker is a late-flowing plant due to high temperature or a short low-temperature period in winter. We planted bulbs with circumferences of 10-18 cm in the greenhouse’s pots, and then transplanted them with buds exposed into phytotrons (30/25, 25/20, and 20/15°C). After planted in an environment (30/25°C) for six weeks, the buds had their diameters rapidly increased and the pedicels extensively elongating over time that demonstrated fast-growing pedicels of plants processed in this environment (30/25°C). Plants processed at 30/25°C, first buds transformed to be in full bloom average number of day 184.3. Plants processed at 25/20°C, first buds transformed to be in full bloom average number of day192.7. Plants processed at 20/15°C had their buds growing slowly. In the experiment for pollen cultivated in vitro, the pollen growing at 20/15°C germinated at the temperature of 30、25 and 20°C, the percentage of germination were 4, 13 and 30, respectively.
Then to improve the quality of cut flowers from riental lily, we took oriental hybrid lily, ‘Medusa’ and ‘Marco Polo’, for differentiation of flower bud and flowering. ‘Medusa’ was planted in boxes to observe differentiation of flower buds, development of floral organs, and vase life of flowering. With primordium having grown originally, the plants completed outer perianth totally developed in week 2, 70% inner perianth developed as well as 30% stamen formed in week 3, and pistil totally developed in week 4. Pedicels extended abruptly between week 8 and week 9 ; flower buds elongated in week 10 ; flowers harvested due to colors changed had the vase life of 13 days. ‘Marco Polo’ with circumferences from 14 to 16cm was dipped in different concentrations of gibberellin, and processed over different periods for later planting ; flower buds growing from bulbs of ‘Marco Polo’ which were dipped in 200 mg l-1 and 300 mg l-1 gibberellin for 10 minutes tended to elongate.
URI: http://hdl.handle.net/11455/29193
其他識別: U0005-2108201000174500
Appears in Collections:園藝學系

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