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Studies on Fruit Characteristic and Genetic Correlation of Pineapple (Ananas comosus (L.) Merr.)
|關鍵字:||pineapple(Ananas comosus );鳳梨;Fruit Characteristic;genetic;correlation;RAPD;genetic marker;果實形質;遺傳;相關性;逢機擴大多形性DNA;遺傳標誌基因||出版社:||園藝學系||摘要:||
利用RAPD分析Smooth Cayenne的7個營養系，結果DNA條帶幾乎相同，得知RAPD無法鑑定Smooth Cayenne的營養系。以同樣的引子進行RAPD分析11個品種，由呈現的條帶可清楚區分為數個叢群，證實RAPD分析可做為鳳梨的品種鑑別之用。
本研究利用RAPD分析台農4~19號的條帶圖譜，繪製成親緣關係之樹狀圖。另外，在OP P10引子的RAPD分析條帶2000 bp的位置上，發現Clone 3607缺少一條明顯的條帶，推測此一條帶與piping type葉型遺傳基因的表現有關。
The research work made use of the current available pineapple (Ananas comosus (L.) Merr.) varieties in Taiwan for the investigation of the characteristics of pineapple plants, capacity of photosynthesis, mineral nutrient content of leaves and its relations with fruit qualities. It was our hope to utilize the related characters as criteria for selecting the desirable hybrid at an early stage of seedling growth and as index for improving the efficiency of plant breeding. In the mean time, RAPD was employed to estimate the genetic similarities among clones and varieties as well as to search for a reliable marker for the selection of hybridized seedlings.
The qualities of pineapple fruits vary with years, seasons and climatic conditions. Results of fruit-quality analyses of different varieties were comparable with those of pineapple-hybridized seedlings line comparative experiments conducted at the Chia-Yi Agricultural Experiment Station.
The characteristics of plant such as leaf numbers, total fresh weights, total leaf areas and total fresh weights of the above ground portion were found to be irrelevant to the fruit qualities. The relationships between the leaf angle and fruit sugar-acid ratio or between the length of the longest leaf and titratable acid of the fruit was insignificant.
The maximum quantum yield and effective quantum yield of pineapple varied among cultivars: highest in Tainung No.13 but lower in Tainung No.19, Tainung No.6 and Pernambuco. Chlorophyll contents also varied among different varieties. The total chlorophyll content related with neither maximum quantum yield nor effective quantum yield, which in turn had no effect on fruit quality. No significant correlation between total chlorophyll content and fruit quality was found. Sugar content tended to increase with increasing chlorophyll concentration, so was the sugar acid ratio(TSS/TA).
The titratable acid of leaves before sunrise was to a certain degree of proportional to the effective quantum yield but with no relations with maximum quantum yield. The titratable acid before sunset was affected neither by the effective quantum yield nor by the maximum quantum yield. The differences in the amount of titratable acids before sunrise and after sunset had no influence upon fruit qualities.
Nutrient elements concentrations of leaves differed among varieties ; nitrogen concentration correlated with total chlorophyll content while magnesium did not. Leaf nitrogen and phosphorus had no effect on fruit quality whereas potassium had negative correlation with fruit sugars and titratable acids. No consistent relationship was found between potassium and fruit sugar-acid ratio.
Leaf calcium showed a significant negative correlation with titratable acids and a positive correlation with sugar-acid ratio but not with fruit sugars. No meaningful relationship was found between magnesium and fruit quality.
RAPD Analysis was used to assay the 7 clones of Smooth Cayenne. Results indicated that bands of DNA obtained were almost identical. In a separate experiment, RAPD was applied to test 11 varieties with same primer, and bands separated could be classified into several clusters. This proves that RAPD can be adopted to identify different varieties of pineapple.
RAPD was also used to map the dendrogram of Tainung No.4 to No. 19, that a family tree could be constructed. Besides, RAPD on the 2,000 bp position of OP P10 primer revealed that Clone 3607 had lost a distinct band. The finding was taken to mean that it resulted in the gene expression of piping leaf characteristic of Clone 3607.
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