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Solid Matrix Priming of Pea, Snap Bean, and Asparagus Bean Seeds
|關鍵字:||Solid Matrix Priming;固體滲調;Pea;Snap Bean;Asparagus Bean;Seed;豌豆;菜豆;豇豆;種子||出版社:||園藝學系||摘要:||
Solid matrix priming of "Wusui" pea, "Seven-inch snow" snap bean, and "Green-pod" asparagus bean seeds with vermiculites and Agro-Lig at 15℃ improved seed germination. Priming treatment was determinated by water uptake of seeds, characteristics of seeds and media, and water potential of seed and media during priming.
Pea, snap bean, and asparagus bean seeds had 15.1%, 10.8%, and 12.2% moisture content; 45.2%, 54.1%, and 55.5% carbohydrate and protein content. Among the three crops, the weight of one hundred pea seeds was the heaviest at 359.75g and asparagus bean was lightest at 119.36g.
When the vermiculite was saturated by water, the water amount was three times of the weight of the vermiculite. Agro-Lig only needed equal amount of water to be saturated. The bulk density, electrical conductivity and pH of the vermiculite were 0.1g/cm3, 0.05mS/cm, and 7.5; they were 0.5g/cm3, 1.25mS/cm, and 4.4 for Agro-Lig.
The seeds of pea, snap bean, and asparagus bean were primed with the vermiculite. Increasing the amount of water in the vermiculite significantly increased the moisture content of seeds during seed priming. The highest moisture content of primed pea, snap bean, and asparagus bean were 51%, 46%, and 53%, respectively. The water content of seeds was increased to the lag phase. During priming, decreasing the amount of water in the vermiculite significantly reduced the released amount of CO2 among three crops. When the water amount for the seeds of three crops was enough, water potential was achieving and maintaining a near-equilibrium between seeds and vermiculites.
The amount of water, the duration of priming, and the size of vermiculite significantly affected the mean germination days of pea seeds. The mean germination days and GT90-10 of snap bean primed seeds were affected by the interaction between the water amount and the duration of priming. The duration of priming affected the germination percentage of asparagus bean seeds.
Pea seeds were primed by the weight ratio of seed:vermiculite #2:0.1%NaOCl=30:15:35 for two days, when it was 15:15:25 for three days in asparagus bean seeds. Snap bean seeds were primed by the weight ratio of seed:vermiculite #3:0.1%NaOCl=20:15:20 for two days. In three crops, priming reduced the mean germination (emergence) days at 15℃, 25℃, and 35℃. In pea seeds, priming increased the germination (emergence) percentage at 15℃ and 35℃. In asparagus bean seeds, priming increased the germination (emergence) percentage at 15℃.
When seeds of the three crops were imbibed at 25℃, the water absorption rate in the phase I of primed seeds was faster than that of unprimed seeds. The length of the lag phase (phase II) of primed seeds was increased by 12 hours in pea seeds, decreased by 12 hours in snap beans, and unchanged in asparagus beans. The conductivity of primed seeds of pea and asparagus bean was decreased as the amount of water in the vermiculite decreased during priming. The conductivity values of the seeds primed with 35ml and 25ml of water were 20uA/seed/4ml, significantly lower than those of unprimed seeds.
The effective priming treatments were concerned with achieving and maintaining a near-equilibrium water potential between seeds and media, while moisture content of seeds achieved the lag phase. The released amount of CO2 from the seeds during priming could be a index of priming. Priming reduced electrolyte leakage and enhanced seed vigor. The germination percentage and germination rate were improved in primed seeds under adverse environmental conditions.
|Appears in Collections:||園藝學系|
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