Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/29399
標題: 扇形文心蘭與文心蘭亞族植物之形態、雜交及其子代之葉片培養
The morphology and hybridization between Erycina pusilla and Oncidiinae and the leaf culture in vitro of hybrid offspring
作者: 劉冠廷
Liu, Kwan-Ting
關鍵字: 扇形文心蘭;Erycina pusilla;雜交;葉片培養;hybrid;leaf culture
出版社: 園藝學系所
引用: 1. 文紀鑾. 2009. 組織培養苗之健康管理. 花卉健康管理研討會專刊:129-135. 2. 邱金春. 2003. 文心蘭盆花是未來產業發展趨勢. 台灣花卉園藝 184:30-35. 3. 邱金春、劉政道. 2004. 文心蘭有性繁殖技術. 豐年 54(24):23-26. 4. 邱金春. 2005. 文心蘭雜交育種技術. 農業世界 267:19-23. 5. 邱金春、王三太. 2011. 遠緣雜交育種障礙及克服策略. 農業試驗所技術服務季刊.台中. 85:19-22 6. 邱翊恬. 2011. 扇形文心蘭試管內開花授粉及與劍葉文心蘭屬間雜交之研究. 國立中興大學園藝學系碩士論文. 台中. 7. 邱翊恬、張正. 2011a. 扇形文心蘭生物學研究概況. 植物種苗 13(1):21-30. 8. 邱翊恬、張正. 2011b. 三種劍葉文心蘭栽培種之雜交潛力和近交弱勢. 興大園藝. 36(1):31-38. 9. 邱翊恬、張正. 2012. 扇形文心蘭與劍葉文心蘭屬間雜交. 台灣園藝 58(2):117-124. 10. 易美秀. 2007. 文心蘭開始轉黃的蒴果適合無菌播種. 台中區農情月刊第 90 期. 11. 易美秀. 2000. 文心蘭授粉、結實與無菌播種之研究. 國立中興大學園藝學系碩士論文. 台中, 136p 12. 易美秀. 2003. 文心蘭雜交育種. 台中區農情月刊. 47:102-115. 13. 易美秀、王寅東. 2010. 文心蘭盆花育種. 台中區農情月刊第134期. 14. 胡正榮. 2001. 文心蘭類之花粉發育、花粉活力、結實及無菌播種之研究. 國立台灣大學園藝學系碩士論文. 台北, 94p. 15. 陳健忠. 1999. 文心蘭之體胚發生與植株再生. 國立台灣大學園藝學系博士論文. 台北, 5p. 16. 黃勝忠、蔡奇助、易美秀. 2002. 利用分子標誌分析rDNA之ITS區域鑑定文心蘭亞族品種. 台中區農業改良場研究彙報 74:1-15. 17. 黃慧宜、朱建鏞. 2009. 芭菲爾鞋蘭無菌培養之建立. 興大園藝 34(1):37-46. 18. 劉冠廷、邱翊恬、張正. 2011. 扇形文心蘭與文心蘭亞族成員之屬間雜交. 植物種苗 13(1):31-39. 19. 蔡奇助、莊畫婷. 2009. 遠緣雜交與分子遺傳鑑定技術在蝴蝶蘭育種之應用潛力. 植物種苗生技 17:37-45. 20. 蔡雅竹. 2010. 紅鶴頂蘭遠緣雜交及花梗培養. 國立中興大學園藝學系碩士論文.台中. 21. Ackerman, J. D., A. Sabat and J. K. Zimmerman. 1996. Seedling establishment in an epiphytic orchid: an experimental study of seed limitation. Oecologia 106:162–98. 22. Baker, M. R. L. and C. R. O. Baker. 2006. Orchid species culture Oncidium/Odontoglossum alliance. 992p., Timber Press, Oregon. 23. Begum, A. A., M. Tamaki, M. Tahara and S. Kato. 1994. Somatic embryogenesis in Cymbidium through in vitro culture of inner tissue of protocorm-like bodies. J. Jpn. Soc. Hort. Sci. (63):419–427. 24. Catling, P. M., V. R. Brownell and L. P. Lefkovitch. 1986. Epiphytic orchids in a Belizean grapefruit orchard:distribution, colonization, and association. Lindleyana 1(3):194-202. 25. Chang, W. C. 2007. In vitro morphogenesis and micro-propagation of orchids. In: W. H. Chen and H. H. Chen (eds), Orchid Biotechnology. World Scientific, Singapore, pp. 45-64. 26. Chang, Y. K., L. A. Blischak, R. E. Veilleux and M. J. Iqbal. 2010. Effects of temperature on gametophytic selection in a Phalaenopsis F1 population. Euphytica 171: 251-261. 27. Chase, M.W. 1986. A reappraisal of the oncidioid orchids. Syst. Bot. 11:477-491. 28. Chase, M.W. 1987. Obligate twig epiphytes: a distinct subset of Neotropical orchidaceous epiphytes. Selbyana 10:24-30. 29. Chase, M.W. and J. D. Palmer. 1997. Leapfrog radiation in floral and vegetative traits among twig epiphytes in the orchid subtribe Oncidiinae. In: T. J. Givnish and K. J. Sytsma, eds. Molecular evolution and adaptive radiation.Cambridge:Cambridge University Press, pp. 331-352. 30. Chase, M.W. and J. S. Pippen. 1988. Seed morphology in the Oncidiinae and related subtribes (Orchidaceae). Syst. Bot. 13(3): 313-323. 31. Chase, M. W., L. Hanson, V. A. Albert, W. M. Whitten and N. H. Williams. 2005. Life history evolution and genome size in subtribe Oncidiinae (Orchidaceae). Ann. Bot. 95:191-199. 32. Chase, M. W., H. W. Norris, M. N. Kurt and W. M. Whitten. 2008. Taxonomic transfers in Oncidiinae to accord with genera Orchidaceae. Lindleyana 21(3): 20-31. 33. Chen, J. T., C. Chen and W. C. Chang. 1999. Direct somatic embryogenesis on leaf explants on Oncidium Gower Ramsey and subsequent plant regeneration. Plant Cell Rep. 19:143-149. 34. Chen, J. T. and P. I. Hong, 2012. Cellular origin and development of secondary somatic embryos in Oncidium leaf cultures. Biol. Plantarum 56(2):215-220. 35. Chen, J. T. and W. C. Chang. 2001. Effects of auxins and cytokinins on direct somatic embryogenesis on leaf explants of Oncidium Gower Ramsey. Plant Growth Regul. 34:229-232. 36. Chen, J. T. and W. C. Chang. 2002. Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium Gower Ramsey. Plant Cell Tiss. Org. Cult. 69:41-44. 37. Chen, J. T. and W. C. Chang. 2000. Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium(Orchidaceae). Plant Sci. 160:87-93. 38. Chen, J. T., Y. J. Su, I. F. Wu, C. M. Chueh, H. L. Kuo and W. C. Chang. 2005. Somatic embryogenesis in Oncidium. Acta Hort. 692:81-83. 39. Chen, J. T. and W. C. Chang. 2004. TIBA affects the induction of direct somatic embryogenesis from leaf explants of Oncidium. Plant Cell Tiss. Org. Cult. 79:315-320. 40. Chen, J. T. and W. C. Chang. 2003a. 1-aminocyclopropane-1-carboxylic acid enhanced direct somatic embryogenesis from Oncidium leaf cultures. Biol. Plantarum 46(3):455-458. 41. Chen, J. T. and W. C. Chang. 2003b. Effects of GA3, ancymidol, cycocel and paclobutrazol on direct somatic embryogenesis of Oncidium in vitro. Plant Cell Tiss. Org. Cult. 72:105-108. 42. Chiu, Y. T., C. S. Lin, and C. Chang. 2011. In vitro fruiting and seed production in Erycina pusilla (L.) N. H. Williams & M. W. Chase. Propag. Ornam. Plants. 11(3):131-136. 43. Dalstrom, S. 2003. Orchids smarter than scientists-an approach to Oncidiinae (Orchidaceae) taxonomy. Lankesteriana 7:33-36. 44. Damon, A. and J. Valle-Mora. 2008. Retrospective spatial analysis of the pollination of two miniature epiphytic orchids with different pollination strategies in a coffee plantation in Soconusco, Chiapas, Mexico. Bot. J. Linn. Soc.158:448-459. 45. Dodson, C. H. and R. L. Dressler. 1972. Two undescribed genera in the Orchidaceae-Oncidiinae. Phytologia 24(4):285-292. 46. Dressler, R. L. 1990. The Orchids. 142-233p., Harvard University Press, London. 47. George, E. F. 2008. Plant Propagation by Tissue Culture. 508p., Basingstoke. 48. Felix, L. P. and M. Guerra. 1999. Chromosome analysis in Psygmorchis pusilla (L.) Dodson and Dressler: the smallest chromosome number known in Orchidaceae. Caryologia 52(3-4):165-168. 49. Felix, L. P. and M. Guerra. 2000. Cytogenetics and cytotaxonomy of some Brazilian species of Cymbidioid orchids. Genet. Mol. Biol. 23(4):957-978. 50. Filonova, L. H. P. V. Bozhkov and S. von Arnold. 2000. Developmental pathway of somatic embryogenesis in Picea abies as revealed by time-lapse tracking. J. Exp. Bot. 51(343):249-264. 51. Freudenstein, J. V. and F. N. Rasmussen. 1999. What does morphology tell us about orchid relationships?-A cladistic analysis. Am. J. Bot. 86(2):225-248. 52. Goh, C. J., M. S. Strauss, and J. Arditti. 1982. Floral induction and physiology in orchids. p.213-241. In:Arditti, J. (eds). Orchid biology-reviews and perspectives. V2. Cornell University Press. 53. Govaerts, R. 2004. World Checklist of Monocotyledons Database in ACCESS: 1-54382. The Board of Trustees of the Royal Botanic Gardens, Kew. 54. Gravendeel, B., S. Ann, J. W. Ferry and A. Schuiteman. 2004. Epiphytism and pollinator specialization: drivers for orchid diversity? Phil. Trans. R. Soc. Lond. B. 359: 1523-1535. 55. Hong, P. I., J. T. Chen and W.C. Chang. 2008. Promotion of direct somatic embryogenesis of Oncidium by adjusting carbon sources. Biol. Plantarum 52(3):597-600. 56. Hormaza, J. I. and M. Herrero. 1992. Pollen selection. Theor. Appl. Genet. 83(6-7):663-672. 57. Karasawa, K. 1989. Oncidium and Odontoglossums. Orchid Atlas vol. 7. Orchid Atlas Society, Tokyo, Japan. 58. Livingston, R. B. 1962. Oncidium pusillum-blooming in flask. Amer. Orchid Soc. Bul. 31: 1007. 59. Mayer, J. L. S., G. C. Stancato and B. Appezzato-Da-Glo΄ria. 2010. Direct regeneration of protocorm-like bodies (PLBs) from leaf apices of Oncidium flexuosum Sims (Orchidaceae). Plant Cell Tiss. Org. Cult. 103:411-416. 60. Mckenzie Black, P. and W. Rittershausen. 1988. The complete book of orchid growing. 160p. Trafalgar Square Pub. North Pomfret, Vt. 61. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497. 62. Namasivayam, P. 2007. Acquisition of embryogenic competence during somatic embryogenesis. Plant Cell Tiss. Org. Cult. 90:1-8. 63. Pan, I. C., D. C. Liao, F. H. Wu, H. Daniell, N. D. Singh, C. Chang, M. C. Shih, M. T. Chan and C. S. Lin. 2012. Complete chloroplast genome sequence of an orchid model plant candidate: Erycina pusilla apply in tropical Oncidium breeding. PLoS One 7(4):e34738. 64. Proctor, H. C. 1998. Effect of pollen age on fruit set, fruit weight, and seed set in three orchid species. Can. J. Bot. 76(3):420-427. 65. Pupulin, F. and G. Merino. 2010. Comparettia sotoana (Orchidaceae: Oncidiinae), a new Ecuadorian species. Lankesteriana 9 (3): 399-402. 66. Rodri’guez-Robles, J. A., E. J. Mele’ndez and J. D. Ackerman. 1992. Effects of display size, flowering phenology, and nectar availability on effective visitation frequency in Comparettia falcata (Orchidaceae). Ann. Bot. 79 (9):1009-1017. 67. Royal Horticultural Society. <http://apps.rhs.org.uk/horticulturaldatabase/orchidregister/> 68. Sacher, R. F., D. L. Mulcahy and R. C. Staples. 1983. Developmental selection during self-pollination of Lycopersicon × Solanum F1 for salt tolerance of F2. In: Mulcahy, D. L., E. Ottaviano (eds) Pollen: biology and implication for plant breeding. Elsevier Press, New York, NY, pp 329–334. 69. Sanford, W. W. 1964. Sexual compatibility relationships in Oncidium and related genera. Amer. Orchid Soc. Bul. 33:1035-1048. 70. Sheehan, T. and M. Sheehan. 1989. Orchid genera illustrated 127-Psygmorchis. Amer. Orchid Society Bul. 58: 24-25. 71. Stort, M. N. S. 1984. Sterility barriers of some artificial F1 orchid hybrids: male sterility. I. microsporogenesis and pollen germination. Amer. J. Bot. 71(3):309-318. 72. Singer, R. B. and S. Koehler. 2003. Notes on the pollination biology of Notylia nemorosa (Orchidaceae): do pollinators necessarily promote cross pollination? J. Plant Res. 116:19-25. 73. Singh, R. J. and T. Hymowitz. 1987. Intersubgeneric crossability in the genus Glycine Willd. Plant Breed. 98:171–173. 74. Tangley, L. 1982. Bee fore sex: Its orchid fragrance. Science 121(8):117. 75. Van Tuyl, J. M. and H. C. M. van Holsteijn. 1996. Lily breeding research in the Netherlands. Acta. Hort. 414:35-45. 76. Vaz, A. P. A., R. D. C. L. Figueiredo-Ribeiro and G. B. Kerbauy. 2004. Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid. Plant Physiol. Biochem. 42:411-415. 77. Vaz, A. P. A. and G. B. Kerbauy. 2000. Effects of mineral nutrients on in vitro growth and flower formation of Psygmorchis pusilla (Orchidaceae). Acta Hort. 520: 149-156. 78. Vaz, A. P. A. and G. B. Kerbauy. 2008. In vitro flowering studies in Psygmorchis pusilla. Glob. Sci. 42:427-432. 79. Von Arnold, S., I. Sabala, P. Bozhkov, J. Dyachok and L. Filonova. 2002. Develpomental pathways of somatic embryogenesis. Plant Cell Tiss. Org. Cult. 69:233-249. 80. Williams, N. H., M. W. Chase, T. Fulcher and M. W. Whitten. 2001. Molecular systematics of the Oncidiinae based on evidence from four DNA sequence regions: expanded circumscriptions of Cyrtochilum, Erycina, Otoglossum, and Trichocentrum and a new genus (Orchidaceae). Lindleyana 16: 113–139. 81. Yang, X. Y. and X. L. Zhang. 2010. Regulation of somatic embryogenesis in higher plants. Crit. Rev. Plant Sci. 29:36-57.
摘要: 
本文探究扇形文心蘭和文心蘭亞族的原種或栽培種之屬間雜交,測試稔實性,並調查性狀,評估其在育種上利用價值,並嘗試建立扇形文心蘭雜交子代之大量繁殖方法。
扇形文心蘭與細枝附生蘭中的數屬具有雜交親合性,包含劍葉文心蘭屬(Tolumnia)、長腺蘭屬(Macradenia)、凹唇蘭屬(Comparettia)等,部分雜交組合可產生種子經無菌播種後可發芽,其中劍葉文心蘭屬與扇形文心蘭屬親合性最高,凹唇蘭次之,長腺蘭最低。扇形文心蘭與凹唇蘭雜交子代種子在播種後 4 週發芽,期間每 1 個月繼代一次,並在繼代的過程有2株雜交子代抽出花梗。
扇型文心蘭與劍葉文心蘭子代之根段、短縮莖及葉片作為培植體,以葉片污染率最低,僅有36.7%,經過誘導後可以在瓶內再生成植株,以再生植株的葉片為次級培植體,進行第二次的誘導,所形成的類原球體經過4週的培養後可以達到2倍的增殖倍率,屬間雜交的實生子代中 Erycina pusilla × Tolumnia Jairak Firm ‘Brown White’ 與 Tolumnia Jairak Firm ‘Brown White’ × Erycina pusilla 或是葉片再生的 Erycina pusilla × Tolumnia Fire Ring 的植株,分別各有5株、5株或10株,經馴化出瓶後皆能存活。

This study is based on Erycina pusilla, using the native species or cultivars in Oncidiinae to cross with Erycina pusilla, in order to test its crossability. Cultivation characteristics of each germplasms were also investigated to evaluate its application value in breeding. Also, this study attempted to set up the mass propagation system of the hybrids.
Several genera in obligate twig epiphyte have crossability with Erycina pusilla, seeds can be formed in some of the crosses, and germinate under asymbiotic conditions. These genus contain Tolumnia, Macradenia, Comparettia and etc. Among those genuses, Tolumnia has the highest crossability with Erycina pusilla, Comparettia has lower and Macradenia has the lowest.
Among all the crosses, hybrid seeds of Erycina pusilla × Comparettia ignea germinated four weeks after sowing, has the fan-like plant morphology. Subculture was carried out each month. During the subculture process two of the six plantlets spiked, but further floral characteristic remains observation.
Root segments, compressed stem and leaf segments of Erycina pusilla × Tolumnia Fire Ring were used as explants. The leaf segments as explants has the lowest contaminated rate among three kinds of explants, only 36.7%. In vitro plantlets could regenerate under induction conditions. Take the leaf of those plantlets as secondary explant to undergo the second induction, PLBs formed from these leaves could achieve two folds of the multiplication rate after 4-week culture. Not only the hybrid seedlings of Erycina pusilla × Tolumnia Jairak Firm ‘Brown White’ and Tolumnia Jairak Firm ‘Brown White’ × Erycina pusilla or the plantlets regenerated from leaf segments of Erycina pusilla × Tolumnia Fire Ring could survive after acclimation, each has five, five and ten plantlets irrespectively, showing the mass propagation system has its feasibility.
URI: http://hdl.handle.net/11455/29399
其他識別: U0005-1308201215003200
Appears in Collections:園藝學系

Files in This Item:
File SizeFormat Existing users please Login
nchu-101-7099032023-1.pdf991.47 kBAdobe PDFThis file is only available in the university internal network    Request a copy
Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.