Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/30470
標題: Mass rearing propagation of Amblyseius womersleyi Schica (Acari: Phytoseiidae) in a closed system
密閉式飼育瓶繁殖溫氏捕植璊Amblyseius womersleyi Schica (Acari: Phytoseiidae)之探討
作者: 黎家興
Li, Chia Hsing
關鍵字: Mass rearing;大量飼育;Amblyseius womersleyi;Tetranychus urticae;溫氏捕植璊;二點葉璊
出版社: 昆蟲學系
摘要: 
Abstract
Amblyseius womersleyi (Schicha) was reared in a closed system in a vial ( 5.6 cm × 3.7 cm diam.) at 26 ± 1℃, 13L:11D and 50%, 60%, 70%, 80% and 90% RH. The highest egg hatching rate (100%) of A. womersleyi was found at 80% RH, which was not significantly different from those tested at 70%, and 90% RH, but was higher than those at 50% and 60%. The optimal relative humidity to preserve the predaceous mites in a vial without prey and water was 90% RH. The days of 50% mortality reached for larvae, protonymphs, adult males and adult females were 2.6, 2.6, 4.4 and 3.7 days, respectively. With sufficient food supply, a high percentage of eggs, larvae, protonymphs and deutonymphs developed were found at 70 - 90 % RH. Relative humidity significantly influences the daily prey consumption rate of adult females of A. womersleyi, but not the daily fecundity. The maximal number of predators (1296 ± 161.7 mites/vial) were reared and reached at day 21, under a closed propagation conditions with daily food supply of 0.01g and 0.02g of eggs and larvae of Tetranychus urticae for 1-14 days and 15-21 days, respectively. The predator densities reached highest ( 1296±161.7 mites/vial ) at day 21. The advantages and superiorities of using closed rearing unit of a vial rather than open artificial stages were compared and discussed.
2. 往昔研究 3
2.1. 捕植蟎、葉蟎及二者間之相互作用 3
2.2. 二點葉蟎與溫氏捕植蟎之重要性 7
2.2.1. 二點葉蟎之重要性 7
2.2.2. 溫氏捕植蟎之重要性 8
2.3. 影響捕植蟎族群增殖力與捕食力之因素 9
2.3.1. 非生物因素 9
2.3.2. 生物因素 13
2.4. 大量飼育捕植蟎 17
3. 材料與方法 24
3.1. 材料 24
3.1.1. 食餌準備 24
3.1.2. 飼育載台及捕植蟎定齡 24
3.1.3. 育蟎瓶、單隻飼育管與給餌裝置 25
3.1.4. 溼度控制盒 26
3.2. 相對溼度對溫氏捕植蟎各齡期之影響 27
3.2.1. 卵孵化率 27
3.2.2. 各齡期蟎體成長百分率 27
3.2.3. 不給餌處理下各齡期蟎體之存活狀態 27
3.2.4. 雌成蟎之捕食量及產卵量 28
3.2.5. 相對溼度及給餌量對育蟎瓶內溫氏捕植蟎族群密度增殖之影響 28
4. 結果與討論 29
4.1. 相對溼度對溫氏捕植蟎各齡期之影響 29
4.1.1. 卵孵化率 29
4.1.2. 齡期發育成功百分率 29
4.1.3. 不給餌處理下各齡期存活狀態 31
4.1.4. 發育期所需時間 32
4.1.5. 雌成蟎每日捕食量及產卵量 33
4.2. 相對溼度對溫氏捕植蟎族群之影響 34
4.3. 不同給餌量對育蟎瓶內捕植蟎族群增長之影響 35
5. 結論 39
6. 參考文獻 ………………………………………………………………………39
中文摘要…………………………………………………………………….……….55
Abstract…………………………………………………………………………..…...56

中文摘要
在育璊瓶之密閉繁殖系統,於26 ± 1℃及13L:11D下,溫氏捕植璊卵於80% RH時具有最高之卵孵化率(100%),其次為90%RH(98.8±2.8%)及70% RH (96.3±3.4%),此三者間差異不顯著。在26±1℃、不給餌及無水密閉條件下,以90% RH下之50% 死亡率到達日數(DD50)為最長(幼璊為2.6天,前若蟎為2.6天,雄成蟎為4.4天及雌成蟎為3.7天);在充足食餌供應及高溼條件(90%、80%及70% RH)下對保存溫氏捕植蟎之卵孵化率、幼蟎、前若蟎及後若蟎發育至下一齡期之存活率較高。於80% RH之溫氏捕植蟎有最高之理論發育成功百分率(100%)及最短之發育期(5.6日)。溫氏捕植蟎雌成蟎之每日捕食量與相對溼度高低成反比,但產卵量受溼度影響不顯著。於26±1℃及80% RH下以密閉系統(3.7 cm diam. × 5.6 cm)大量繁殖溫氏捕植蟎,以10隻雌成蟎及10隻雄成蟎為起始族群時,每日給餌量以分為二階段給餌最為合適,即第1~14天每日給餌0.01g葉蟎「卵-幼蟎」,第15~27天每日給餌0.02g葉蟎「卵-幼蟎」,並於第21日獲得最高族群密度(1296±161.7(蟎/瓶))。
URI: http://hdl.handle.net/11455/30470
Appears in Collections:昆蟲學系

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