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|標題:||Rational Rearing Processes, Harvest, Density Sampling and Monitoring of Amblyseius womersleyi Schicha by Microfeeder and Enclosed Unit
利用密閉瓶及微量給餌器大量飼育Amblyseius womersleyi Schicha之合理飼育流程、收穫、密度取樣及監控技術
|關鍵字:||溫氏捕植;amblyseius womersleyi;大量飼育;二點葉;捕植;mass rearing;Tetranychus urticae;predatory mite||出版社:||昆蟲學系||摘要:||
密閉式飼育瓶以微量給餌器裝填 0.005g、0.01g 及 0.02g 的二點葉卵皆精準，小於 0.02g 給餌量顯著精確（t = 0.1538~1.7480），但 0.03g 則於裝填及給餌量皆有顯著差異（t = 22.3670~23.0071）。微量給餌器提供重複而正確的低給餌量（< 0.02g），然對 0.03g 則需改進其裝填及給餌精準度。每飼育瓶內接 10 隻定齡 Amblyseius womersleyi Schicha 雌捕植，每日給餌量依飼育期之增加而調整：0~3 日給 0.001g，4~5 日給 0.002g，6~9 日給 0.005g，10~15 日給 0.01g，16~19 日給 0.02g 及 20 日以後給 0.03g，於 21 日平均每飼育瓶內可收穫 2,021 隻捕植；另於 23 日後給 0.05g 可收穫 3,150 隻捕植；該等飼育法為目前所知最小密閉飼育單元及最短飼育期內可獲之最高捕植數量。溫氏捕植族群增殖之迴歸方程式，評估得族群密度之增殖自指數型增殖轉換成 S 型增殖的時間點，提供調整每日給餌量及其時機。飼育瓶內溫氏捕植因初期低給餌量使雄性比居高及年齡結構呈不穩定之現象，可因中後飼育期給餌量之增加而提高族群雌性比及穩定年齡結構，進而免於性比及年齡結構影響捕植族群的增殖率及收穫量。花豆葉粉為填充物對捕植族群數量增殖並無影響，但可穩定飼育瓶內濕度性及減少收穫或分裝捕植時因葉絲線糾纏的困擾。自飼育瓶內壁調查計取部分捕植數量的非破壞性取樣技術可精確評估瓶內捕植密度，本取樣法雖已提供監控瓶內族群密度之方法，但仍須人力操作。二氧化碳受捕植密度之每小時增加率可評估密閉飼育瓶內的捕植密度，且精確度高；此評估法提供自動監控捕植數量之空間及生產流程中捕植生產量的控制機制。總之，本研究密閉式飼育瓶、微量給餌器、每日給餌量、飼育期及收穫量等成果，提供了穩定產量的半自動大量繁殖溫氏捕植技術。非破壞性取樣法及二氧化碳評估密閉飼育瓶內溫氏捕植密度技術，除有效監控生產流程中之數量及收穫量，尚可隨時調整給餌量之依據。
The actual feeding rate was done by the micro-feeding apparatus to the rearing chamber. Feeding 0.005g, 0.01g and 0.02g of Tetranychus urticae eggs for mass rearing of Amblyseius womersleyi in a rearing bottle(12 cm × 5.3 cm dia.), we found these feeding were no difference to designed setup (t = 0.1538~1.7480), but 0.03g feeding was significautly difference (t = 22.3670~23.0071). Daily prey eggs offered rationally to gain a better and higher harvested density of predator per bottle is 0.001g at days 0~3, 0.002g at days 16~19, and 0.03g at days 20~21. By these daily prey quotas, we harvested an average 2,021 predators/bottle where we also harvested an average of 3,150 predators/bottle with additional 2 days rearing and feeding 0.05g prey eggs. It is the highest amount of predators harvested in a smallest unit and a shortest rearing duration of the methods. Population growth of the predator in the rearing bottle was an exponential growth function for 0~15 days and then it transformed to sigmoid function. So there were a carrying capacity for the predator population and a refraction point for each estimated equation that was used to adjust the daily ration of prey to the predator. The lower prey ration favors male ratio at early rearing period in the bottle, wherever the daily prey ration is removed it favors female sex ratio. So it was the unstable age structure at early rearing period and low prey ration, but it stabilized with higher daily prey ration. Powder of bean leaves affects not the population growth rate, but stabilizes the relative humidity and removes the difficulties caused by spider mite webbing the harvest and packing processes. Nondestructive sampling method accurately estimates density of predator in a rearing bottle, while the relative density estimated by a change rate of CO2 concentrations also develops CO2 estimation technique, which opens a way to develop automatic monitoring system. Mass rearing of A. womersleyi with enclosed bottle unit, micro-feeding apparatus for accurate daily food supply, daily ration of prey, prey rearing duration and timing of harvest provide a stable and confident CO2 estimation, therefore we could control and monitor the predator's densities during the rearing period which renders us a chance to readjust the daily food ration and manipulate or modify the rearing condition for a better productivity.
|Appears in Collections:||昆蟲學系|
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