Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/30560
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dc.contributor.advisorChain-Ing Shihen_US
dc.contributor.advisor施劍鎣zh_TW
dc.contributor.authorChiang, Chung-Hengen_US
dc.contributor.author蔣仲桓zh_TW
dc.date2004zh_TW
dc.date.accessioned2014-06-06T07:39:39Z-
dc.date.available2014-06-06T07:39:39Z-
dc.identifier.urihttp://hdl.handle.net/11455/30560-
dc.description.abstract密閉式飼育瓶以微量給餌器裝填 0.005g、0.01g 及 0.02g 的二點葉卵皆精準,小於 0.02g 給餌量顯著精確(t = 0.1538~1.7480),但 0.03g 則於裝填及給餌量皆有顯著差異(t = 22.3670~23.0071)。微量給餌器提供重複而正確的低給餌量(< 0.02g),然對 0.03g 則需改進其裝填及給餌精準度。每飼育瓶內接 10 隻定齡 Amblyseius womersleyi Schicha 雌捕植,每日給餌量依飼育期之增加而調整:0~3 日給 0.001g,4~5 日給 0.002g,6~9 日給 0.005g,10~15 日給 0.01g,16~19 日給 0.02g 及 20 日以後給 0.03g,於 21 日平均每飼育瓶內可收穫 2,021 隻捕植;另於 23 日後給 0.05g 可收穫 3,150 隻捕植;該等飼育法為目前所知最小密閉飼育單元及最短飼育期內可獲之最高捕植數量。溫氏捕植族群增殖之迴歸方程式,評估得族群密度之增殖自指數型增殖轉換成 S 型增殖的時間點,提供調整每日給餌量及其時機。飼育瓶內溫氏捕植因初期低給餌量使雄性比居高及年齡結構呈不穩定之現象,可因中後飼育期給餌量之增加而提高族群雌性比及穩定年齡結構,進而免於性比及年齡結構影響捕植族群的增殖率及收穫量。花豆葉粉為填充物對捕植族群數量增殖並無影響,但可穩定飼育瓶內濕度性及減少收穫或分裝捕植時因葉絲線糾纏的困擾。自飼育瓶內壁調查計取部分捕植數量的非破壞性取樣技術可精確評估瓶內捕植密度,本取樣法雖已提供監控瓶內族群密度之方法,但仍須人力操作。二氧化碳受捕植密度之每小時增加率可評估密閉飼育瓶內的捕植密度,且精確度高;此評估法提供自動監控捕植數量之空間及生產流程中捕植生產量的控制機制。總之,本研究密閉式飼育瓶、微量給餌器、每日給餌量、飼育期及收穫量等成果,提供了穩定產量的半自動大量繁殖溫氏捕植技術。非破壞性取樣法及二氧化碳評估密閉飼育瓶內溫氏捕植密度技術,除有效監控生產流程中之數量及收穫量,尚可隨時調整給餌量之依據。zh_TW
dc.description.abstractThe actual feeding rate was done by the micro-feeding apparatus to the rearing chamber. Feeding 0.005g, 0.01g and 0.02g of Tetranychus urticae eggs for mass rearing of Amblyseius womersleyi in a rearing bottle(12 cm × 5.3 cm dia.), we found these feeding were no difference to designed setup (t = 0.1538~1.7480), but 0.03g feeding was significautly difference (t = 22.3670~23.0071). Daily prey eggs offered rationally to gain a better and higher harvested density of predator per bottle is 0.001g at days 0~3, 0.002g at days 16~19, and 0.03g at days 20~21. By these daily prey quotas, we harvested an average 2,021 predators/bottle where we also harvested an average of 3,150 predators/bottle with additional 2 days rearing and feeding 0.05g prey eggs. It is the highest amount of predators harvested in a smallest unit and a shortest rearing duration of the methods. Population growth of the predator in the rearing bottle was an exponential growth function for 0~15 days and then it transformed to sigmoid function. So there were a carrying capacity for the predator population and a refraction point for each estimated equation that was used to adjust the daily ration of prey to the predator. The lower prey ration favors male ratio at early rearing period in the bottle, wherever the daily prey ration is removed it favors female sex ratio. So it was the unstable age structure at early rearing period and low prey ration, but it stabilized with higher daily prey ration. Powder of bean leaves affects not the population growth rate, but stabilizes the relative humidity and removes the difficulties caused by spider mite webbing the harvest and packing processes. Nondestructive sampling method accurately estimates density of predator in a rearing bottle, while the relative density estimated by a change rate of CO2 concentrations also develops CO2 estimation technique, which opens a way to develop automatic monitoring system. Mass rearing of A. womersleyi with enclosed bottle unit, micro-feeding apparatus for accurate daily food supply, daily ration of prey, prey rearing duration and timing of harvest provide a stable and confident CO2 estimation, therefore we could control and monitor the predator's densities during the rearing period which renders us a chance to readjust the daily food ration and manipulate or modify the rearing condition for a better productivity.en_US
dc.description.tableofcontents1. 前言 1 2. 前人研究 3 2.1. 葉之崛起 3 2.2.葉的防治 3 2.3. 捕植之發育、繁殖、性比及競爭 5 2.3.1. 溫度、溼度及光照對捕植之影響 5 2.3.2. 捕植之雌雄性比 6 2.3.3. 捕植的種內與種間競爭 7 2.4. 大量飼育捕植 7 2.4.1. 大量飼育捕植之過程 7 2.4.2. 飼育捕植所需之食物 8 2.4.3. 捕植飼育之單元 8 2.4.4. 評估單元上捕植之族群數量 9 2.4.5. 大量飼育捕植之記錄 10 3. 材料與方法 11 3.1. 食餌之準備 11 3.1.1. 花豆種植方法 11 3.1.2. 飼育葉之方法 11 3.1.3. 葉食餌之準備 12 3.2. 捕植試材之準備 12 3.2.1. 捕植之定齡 12 3.2.2. 密閉飼育瓶之製作 13 3.2.3. 溫氏捕植接入飼育瓶之方法及飼育條件 13 3.3. 給餌方法、給餌量及填充物 13 3.3.1. 每日給餌量 13 3.3.2. 填充物之準備與給予方法 14 3.3.3. 微量給餌器精確度之測定 14 3.3.4. 食餌餵食之方法 15 3.4. 計算密閉飼育瓶內捕植族群數量方法 15 3.4.1. 破壞性計算法 15 3.4.2. 非破壞性取樣單元規劃之方法 16 3.4.3. 非破壞性取樣操作流程 16 3.5. 捕植族群數量之分析 16 3.6. 二氧化碳變化量測定方法 17 4. 結果 18 4.1. 微量給餌量精確度測定 18 4.2. 給餌量及花豆葉粉填充物對捕植族群數量之影響 18 4.2.1. 於 9 日內低給餌量對捕植族群數量之影響 18 4.2.2. 於 10 日後不同給餌量對捕植數量之影響 18 4.2.3. 添加花豆葉粉對捕植族群之影響 19 4.3. 給餌量及飼育期對捕植族群年齡結構之影響 19 4.4. 給餌量及飼育期對捕植成性比之影響 20 4.5. 食餌匱乏對捕植族群內之影響 20 4.6. 累積給餌量轉換捕植族群數量 20 4.7. 擬合生長模式 21 4.8. 非破壞性取樣之精準度 22 4.8.1. 重複取樣計算捕植族群密度之精準度 22 4.8.2 每小時二氧化碳濃度改變量及每飼育瓶內捕植密度之關係 22 5. 討論 23 5.1. 自動給餌器精準度測試 23 5.2. 於前 9 日內低給餌量對捕植族群數量之影響 23 5.3. 不同給餌量及飼育期對捕植族群年齡結構之影響 24 5.4. 不同給餌量對捕植成性比之影響 24 5.5. 食餌匱乏推論及其對捕植族群之影響 26 5.6. 增殖模式之計算與收穫期之評估 27 5.7. 花豆葉粉對捕植繁殖量及收穫捕植之操作影響 28 5.8. 最適給餌量及收穫期之關係 29 5.9. 添加花豆葉粉對捕植族群之影響 29 5.10. 非破壞性取樣之利用 30 5.10.1. 非破壞性取樣評估飼育瓶內捕植族群之密度 30 5.10.2. 利用二氧化碳變化率評估捕植數量 31 6. 結論 33 7. 參考文獻 36 中文摘要 49 ABSTRACT 51zh_TW
dc.language.isoen_USzh_TW
dc.publisher昆蟲學系zh_TW
dc.subject溫氏捕植zh_TW
dc.subjectamblyseius womersleyien_US
dc.subject大量飼育zh_TW
dc.subject二點葉zh_TW
dc.subject捕植zh_TW
dc.subjectmass rearingen_US
dc.subjectTetranychus urticaeen_US
dc.subjectpredatory miteen_US
dc.titleRational Rearing Processes, Harvest, Density Sampling and Monitoring of Amblyseius womersleyi Schicha by Microfeeder and Enclosed Uniten_US
dc.title利用密閉瓶及微量給餌器大量飼育Amblyseius womersleyi Schicha之合理飼育流程、收穫、密度取樣及監控技術zh_TW
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairetypeThesis and Dissertation-
Appears in Collections:昆蟲學系
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