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標題: 機械化大量飼育二點葉之研究
Automation of mass rearing for Tetranychus urticae Koch
作者: 蘇紀昌
Su, Chi-Chang
關鍵字: mass rearing;大量飼育;automation;flooding culture;air-drying;自動機械化
出版社: 昆蟲學系
大量飼育寄主植物(花豆Phaseolus vulgaris L)及二點葉(Tetranychus urticae Koch)之過程中,測定四種浸種催芽方式、二種植株種植法、六種種植密度、三種接種密度及二種收穫後卵之處理方式。機械式浸種法之發芽率達88 % 以上。發芽之豆種以每塑膠籃(孔徑1×0.5 cm,網底32×25 cm,籃緣30×25 cm,高9 cm)30顆之密度種植,存活率可達91 %,每塑膠籃之豆葉面積為3994.9 cm2 。葉之產量與寄主植物之葉面積相關而與種植密度無關。每塑膠籃接種0.03 g 二點葉成-若可產生0.2 g之二點葉,且收穫高原期長達五天。接種0.05 g 二點葉成-若/塑膠籃則因接種量便已超過植株所能負荷,無法達到縮短收穫期及增加產量。二點葉經機械式葉收穫機以強力水柱沖洗至分析篩中(網目100及170),其中卵及幼被收集於170網目之分析篩,成及若於網目100之分析篩,再經由氣流(100ι/ min.)將其吹乾。此種操作較以往人工操作節省四到六倍之時間。二點葉之卵可經UV或微波照射使其不孵化以供純化之捕植飼育。使用氣動式微量葉接種機以供植株接種二點葉或供捕植大量飼育時定量食餌之供給。

The mass rearing of Tetranychus urticae Koch included host plant seeding, spider mite inoculation and mites harvesting with water-jet washing and extra water removing by air-jet. The lima bean seeds (500 seeds) were evenly spreaded on the floor of basket (32cm25cm with 10.5cm holes) with a water-submerging system to produce 88.0 % to 89.6 % of germination rate and 91% of survival rate of seedlings at a density of 30 seeds per basket. The total leaf area produced by 20-d plants with a submerging system was 3,995 cm2. While aero-planting with water spraying system was also tested but produced a low survival rate (73 %) and a low leaf area (1,881 cm2). We inoculated 0.03 g instead of 0.01 g of T. urticae adults and nymphs per basket to produce a higher amount of T. urticae (0.2 g / basket) in a shorter rearing period (11~13 d) and longer harvesting duration with large spider mites (5 days). Inoculating 0.05 g / basket of adults and nymphs, we obtained a low number of T. urticae due to the early senescence of plants. The T. urticae were washed from bean leaves down into air-turbulence compartments of 100 and 170 meshes / inch by a water-jet washing machine. The extra water on adults-nymphs and egg-larvae of T. urticae were removed by air-turbulence in this compartment in which the mites were separate individually from the tangled balls. The sterile T. urticae were obtained from UV or microwave radiation and then refrigerated before feeding to the predators. An air-jet micro-feeding-gun designed for T. urticae to mass rear predaceous mites on a flat universe as also introduced here-in. The procedures of non-soil and hydroponics planting host plant with even and unique host plants (in terms of leaf area and stem strengthness) and stable spider mites production available. In addition , water-washing with auto-collecting and separating the mites with air-turbulence to remove extra water for refrigeration also makes the prey food available at a longer period which makes mass rearing predaceous mites possible.
Appears in Collections:昆蟲學系

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