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|標題:||Expression of BdmGC-1 in three imaginal discs of the
oriental fruit fly, Bactrocera dorsalis (Hendel)
|關鍵字:||Bactrocera dorsalis;鳥苷酸環化酶成蟲盤;BdmGC-1;imaginal disc||出版社:||昆蟲學系所||引用:||Bredt, DS and Snyder, SH (1994) Nitric oxide: a physiologic messenger molecule. Annu Rev Biochem 63: 175-195. Chang, JC, Yang, RB, Adams, ME and Lu, KH (2009) Receptor guanylyl cyclases in Inka cells targeted by eclosion hormone. Proc Natl Acad Sci U S A 106: 13371-13376. Chang, JC, Yang, RB, Chen, YH and Lu, KH (2006) A novel guanylyl cyclase receptor, BdmGC-1, is highly expressed during the development of the oriental fruit fly Bactrocera dorsalis (Hendel). Insect Mol Biol 15: 69-77. Chiu, HT (1987) Studies on the improvement of mass rearing for oriental fruit flies. Plant Prot Bull 20: 87-92. Davies, SA (2006) Signalling via cGMP: lessons from Drosophila. Cell Signal 18: 409-421. Duda, T and Sharma, RK (2008) ONE-GC membrane guanylate cyclase, a trimodal odorant signal transducer. Biochem Biophys Res Commun 367: 440-445. 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Guanylyl cyclases (GCs), kind of enzyme-linked receptor proteins, play important physiological functions, including metamorphosis, olfactory organ and memory, in insects. The BdmGC-1, a receptor form membrane guanylyl cyclase of Bactrocera dosalis (Hendel), has been proved its relationship with eclosion hormone. In this study, we furtherfound that the BdmGC-1 was expressed in the B. dorsalis imaginal discs of wings, legs and compound eyes. Real-time PCR analysis revealed that new (1-day-old) puparium exhibited higher expression level during metamorphosis. Moreover, immunofloruscent staining showed that the BdmGC-1 signals were located in whole imaginal discs. Furthermore, recent studies have showed that p-21 activated protein (PAK) can regulate the mammalian GC activity. Hence, we used a 280-bp BdPAK, cloned from B. dorsalis based on PAK conserved regions, to analyze its expression and location. The results of both real-time PCR analysis and immunofloruscent staining showed that BdPAK has similar expression profiles with BdmGC-1, implying that there some kind of relationships are existed between these two proteins. In summary, BdmGC-1 is indeed expressed in the B. dorsalis wing, compound eye and leg discs and the newly pupated purarium have the higher expression level of BdmGC-1. However, functional interaction of BdmGC-1 with BdPAK has not yet been understood.
鳥苷酸環化酶（guanylyl cyclase, GC）為昆蟲體內一重要受器蛋白（receptor protein），許多生理功能都與其相關，例如變態、嗅覺和記憶等。東方果實蠅（Bactrocera dorsalis (Hendel)）鳥苷酸環化酶，BdmGC-1，已被證實為羽化激素（eclosion hormone）之受器蛋白，並發現其可能與昆蟲蛻皮行為有關。本研究更進一步發現BdmGC-1亦存在東方果實蠅幼蟲體內的翅成蟲盤（wing disc）、眼成蟲盤（eye disc）和足成蟲盤（leg disc）上。以real-time PCR 分析發現，蛹期第一天之翅、眼和足成蟲盤均有較高的BdmGC-1 表現量；以免疫螢光染色（immunofluorescent stain）分析BdmGC-1 在翅、眼和足成蟲盤表現位置，結果顯示其分布位於整個成蟲盤上。另外，從前人研究已知p-21 activated protein（PAK）在哺乳動物上可調節GC 活性。因此，以PAK 保守區域選殖得到一長度280 bp 的東方果實蠅部分PAK 序列，且命名為BdPAK。偵測其基因表現量和BdPAK螢光染色之結果顯示，BdPAK 之表現與位置與BdmGC-1 皆類似，表示BdPAK 與BdmGC-1可能有某種關聯。綜合上述結果可知，BdmGC-1 表現在翅、眼和足成蟲盤上並於蛹期第一天有較高表現，且可能與BdPAK 有某種關聯。
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