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標題: 一氧化氮自由基在菸草過敏性反應中所扮演之角色
Role of Nitric Oxide Free Radical in the Hypersensitive Reaction of Tobacco
作者: 周俊吉
Chou, Chun-Chi
關鍵字: tobacco;菸草;Ralstonia solanacearum;hypersensitive response;nitric oxide;desferal;PARS;青枯病菌;過敏性反應;一氧化氮
出版社: 植物病理學系
激活態氧 (Reactive oxygen species, ROS) 在植物主動防禦反應過程中
(nitric oxide radical, NO‧),亦為參與植物不親和性反應作用過程之
關鍵分子。NO‧ 生合成作用的前驅物質L-arginine,於室溫下,只要1
mM以上濃度對 Ralstonia solanacearum Ps61- 無毒力菌株於台菸五號菸
葉上所造成之過敏性反應壞疽現象即有明顯促進與提早作用,在24 ℃ 以
病菌Ps61- 注射濃度達 7.0X107 cfu/ml 以上時方可表現,且以下位葉促
進效果較為明顯,於生體外,L-arginine對於Ps61- 並無致死能力,在菸
L-arginine結構類似物如NMA、NAME 及can. 等及類似 guanidino group
結構之 methylguanidine 及 aminoguanidine 則均可延遲或減低過敏性
壞疽現象的發生,其中並以can. 的效果最佳,唯can. 對壞疽病徵呈現的
延遲效果可被 L-arginine 回復;過敏性反應過程中,測量菸草葉片細胞
組織間液,可偵測得 NO2- 含量持續提高的現象;於密閉三角瓶中,接種
Ps61- 之葉盤亦可明顯偵測到 NO‧ 氣體的形成,綜合上述生理和生化證
據,NO‧ 之生合成作用確為伴隨菸草過敏性反應過程發生之現象,其對

The production of reactive oxygen species (ROS) has
beenimplicated to be the determinative factor contributing to
the signaling of the active defense mechanism and the killing of
the invading pathogen. The role of ROS in hypersensitive
response (HR) associated rapid necrogenic tissue collapsing
remained largely to be explored. In this investigation, using
the incompatible host-parasite interaction of tobacco
(Nicotinana tabacum cv. TT-5) and Ralstonia solanacearum (Ps61-
isolate), it was demonstrated that production of nitric oxide
radical (NO‧), one of the key role factors known in immune
response and many other essential physiological function of
animal system, is a critical factor involved in the necrogenic
hypersensitive response. At room temperature (25-28℃), the
supplementation of 1mM (or above) L-arginine, the precursor of
NO‧ biosynthesis, enhanced greatly the earliness and severity
of HR related necrosis caused by the artificial inoculation of
Ps61-. The stimulating effect was even more prominent at 24℃
or a lower temperature. It required the challenge of Ps61- at
concentration no less than 7.0x107 cfu/ml; and the response was
usually more prominent among lower mature leaves. The
supplementation of L-arginine, however, did not affect the
survival nature of Ps61- within the tobacco tissue undergoing
HR. The application of L-arginine analogues like NMA, NAME and
can., compounds with guanidino group like methyl-guanidine or
aminoguanidine, all appeared to be greatly effective in
retarding or reducing the necrosis severity, can. was among them
the most effective; the supplementation of L-arginine reversed
the effect of can. when applied at an equal concentration. An
increased production of nitrite reflecting the production of NO
‧ was consistently detected by Greiss reagent method from the
intercellular fluid of Ps61- inoculated tobacco leaf tissue
undergoing HR. The production of gaseous NO‧ was also detected
by an ozone-mediated fluorescence technique from tobacco leaf
disks immersion inoculated with Ps61-. The accumulated evidence
indicates clearly that the production of NO‧ is a physiological
response accompanied with HR which appeared to be one of the
determinative factors contributing to the rapid necrotic tissue
Appears in Collections:植物病理學系

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