Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/30814
標題: 甲基茉莉花酸之施用對蘿蔔及黃條葉蚤(Phyllotreta striolata)的影響
The effects of exogenous methyl jasmonate application induced defense responses in radish and its effects on flea beetle, Phyllotreta striolata
作者: 陳韋辰
Chen, Wei-Chen
關鍵字: 茉莉花酸;Phyllotreta striolata;誘導防禦反應;蛋白酶抑制子;多酚氧化酵素;硫配醣體;jasmonic acid;induced resistance;proteinase inhibitors, PIs;polyphenol oxidase, PPO;glucosinolates, GLS
出版社: 昆蟲學系所
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摘要: 
當植物遭受植食者危害時,會採取防禦措施來降低植食者造成的傷害,而這些反應往往都是多元化的,其中化學防禦物質為主要的方式。控制這些化學物質的主要關鍵為茉莉花酸(jasmonic acid, JA),茉莉花酸屬於一種植物激素能調控植物的誘導防禦反應。而前人研究指出外施茉莉花酸於植物上,可誘導植物產生防禦反應。更有研究指出施用茉莉花酸於植物不同部位時,能夠誘導出不同的化學防禦物質的種類。本試驗利用甲基茉莉花酸(methyl jasmonate, MeJA)施用於蘿蔔不同部位時,來探討處理甲基茉莉花酸後對植物誘導反應的影響,此外也觀察處理後植物對黃條葉蚤(Phyllotreta striolata)成蟲取食表現及其幼蟲存活率的影響。試驗共有四個處裡,分別施用1.5 mM的甲基茉莉花酸溶液於地上部組織、地下部組織、地上與地下部共同處理與對照組。於噴灑甲基茉莉花酸後的第零、一、三、五、七與十天進行葉片的化學物質分析,包括胰蛋白酶抑制子(trypsin inhibitor, TI)、多酚氧化酵素(polyphenol oxidase, PPO)、氮含量(nitrogen)與黑芥籽油(sinigrin),並進行黃條葉蚤成蟲葉面積取食量與幼蟲存活率的試驗。結果發現,於葉面積取食測試方面,黃條葉蚤對於施用甲基茉莉花酸的處理組相較於對照組,於第三、五、七天與十天的取食面積有明顯下降的趨勢。幼蟲存活率在地上地下共同處理組有顯著下降,地下部處理組相較於地上部處理組有延遲羽化的情形。蘿蔔經甲基茉莉花酸誘導後,分別於第5天與第1天後其胰蛋白酶抑制子(TI)與黑芥籽油(sinigrin)有顯著上升¬,而不同部位處理的結果則無顯著的差異。因此由試驗結果得知,蘿蔔經外施甲基茉莉花酸後,能誘導化學防禦物質的改變,並對黃條葉蚤的生長表現造成影響,未來在應用方面可以更深入的探討。

When plants injured by attacker, they would use some strategies to reduce the damage caused by herbivory, and these reactions are diversification; but chemical defense compound maybe the main way. One key compound to controlling of production of chemical defense compound is jasmonic acid (JA). The jasmonic acid is a plant hormone which regulates plant’s induced resistance. Previous studies pointed out that exogenous methyl jasmonate (MeJA) can induce plant defense response. In addition, some studies indicated that applying jasmine acid in different parts of plant can induce different types of chemical defense compound. In this study, applying methyl jasmonate at different parts of radish plants to explore the effects on plant induced resistance and to find the influence on the flea beetle (Phyllotreta striolata) adult feeding performance and larva survival. The treatment included applying the 1.5 mM methyl jasmonate solution on above-ground, below-ground, both above- and below-ground, and control group. Chemical analysis of the leaf on 0, 1st, 3rd, 5th, 7th days and 10th day after applying methyl jasmonate and included trypsin inhibitor (TI), polyphenol oxidase (PPO), nitrogen content, and sinigrin. In addition, the treated plant was used to test effects on flea beetle adults feeding and larva survival rate. The results showed that the flea beetle feeding area decreased significantly on 3rd, 5th, 7th days and 10th day. Survival rate of larva significantly decline in the above and below-ground treatment. The below-ground treatment reveals the delayed eclosion compared to above-ground treatment. In addition, the results also showed that methyl jasmonate can induce trypsin inhibitor (TI) and sinigrin significantly; but have no effect on different plant parts. In summary, the results indicate that exogenous methyl jasmonate on radish can change the chemical defense compound, and affect flea beetle growth performance.
URI: http://hdl.handle.net/11455/30814
其他識別: U0005-2008201210181800
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