Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/30949
標題: 台灣產胡瓜尖鐮胞菌之病原性、營養體親和群以及逢機增幅多形性DNA特性
Characterization of Fusarium oxysporum isolates obtained from cucumber in Taiwan by pathogenicity, VCG and RAPD
作者: 黃明進
Huang, Ming-Jin
關鍵字: forma specialis;生理分化型;Fusarium wilt;root and stem rot;vegetative compatibility group;random amplification of polymorphic DNA;萎凋病;根腐及莖腐病;營養體親和群;逢機增幅多形性DNA
出版社: 植物病理學系所
引用: 沈家昇。2001。利用抑病土中無病原性尖鐮孢菌防治胡瓜萎凋病。國立中興大學植物病理學系第三十一屆碩士論文。台中市。 林益昇、張宏仁。1980。胡瓜鐮刀菌萎凋病之初報。植保會刊 22: 437頁(摘要) 林益昇、黃家興、郭孟祥。1996。台灣苦瓜萎凋病之發生與傳播。植物病理學會刊。5: 38-46。 陳任芳。1999。胡瓜萎凋病之鑑定及篩選無病原性尖鐮胞菌之防治研究。國立中興大學植物病理學系第二十九屆碩士論文。台中市。 黃仲生、楊玉茹。1992。中國黃瓜枯萎病菌致病性小種鑑定。中國植物病理學報22: 204。 梁繼農、王連榮、伊長清、朱國強。1996。南通市黃瓜死藤病原鑑定與發生規律。中國上海農業學報。12: 90-94。 孫守恭、黃振文。1996。台灣植物鐮胞菌病害。世維出版社。台中。170頁。 Agrios, G. N. 2005. Plant Pathology (fifth edition), Page 134. Academic Press, California, USA. 922p. Ahmad, S., and Fliege, F. 1981. Effect of incubation temperatures on growth of Fusarium oxysporum Schlecht f. sp. cucumerinum Snyder and Hansen isolates. Journal of Agricultural Research 19: 45-48. Ahn, I. P., Chung, H. S., and Lee, Y. H. 1998. Vegetative compatibility groups and pathogenicity among isolates of Fusarium oxysporum f. sp. cucumerinum. Plant Dis. 82: 244-246. Appel, D. J., and Gordon, T. R. 1994. Local and regional variation in populations of Fusarium from agricultural field soils. Phytopathology 84: 786-791. Armstrong, G. M., and Armstrong, J. K. 1978. Formae speciales and races of Fusarium oxysporum causing wilts of the Cucurbitaceae. Phytopathology 68: 19-28. Armstrong, G. M., and Armstrong, J. K. 1981. Fusarium: Disease, Biology and Taxonomy. In Anonymous, Formae speciales and races of Fusarium oxysporum causing wilt disease, Pages 391-399. The Pennsylvania University Press, University Park. Assigbetse, K. B., Fernandez, D., Dubois, M. P., and Geiger, J. P. 1994. Differentiation of Fusarium oxysporum f. sp. vasinfectum race on cotton by random amplified polymorphic DNA (RAPD) analysis. Phytopathology 84: 622-626. Bae, Y. S., Kim, H. K., and Park, C. S. 1990. An improved methods for rapid screening and analysis methods of root colonizing biocontrol agents. Korean J. Plant Pathology 6: 325-332. Bentley, S., Pegg, K. G., and Dale, J. L. 1995. Genetic variation among a world-wide collection of isolates of Fusarium oxysporum f. sp. cubense analyzed by RAPD-PCR fingerprinting. Mycol. Res. 11: 1378-1384. Cerkauskas, R. F., and Brown, J. 2001. First report Fusarium stem and root rot of greenhouse cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum in Ontario. Plant dis. 85: 1028. Correll, J. C., Klittich, C. J. R., and Leslie, J. F. 1987. Nitrate nonutilizing mutants of Fusarium oxysporum and their use in vegetative compatibility tests. Phytopathology 77: 1640-1646. Correll, J. C. 1991. The relationships between formae speciales, races, and vegetative compatibility groups in Fusarium oxysporum. Phytopathology 81: 1061-1064. Crowhurst, R. N., Hawthorne, B. T., Rikkerink, E. H. A., and Templeton, M. D. 1991. Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA. Current Genetics 20: 391-396. Crowhurst, R. N., King, F. Y., Hawthorne, B. T., Sanderson, F. R., and Choi-Pheng, Y. 1995. RAPD characterization of Fusarium oxysporum associated with wilt of angsana (Pterocarpus indicus) in Singapore. Mycol. Res. 99: 14-18. Elias, K. S., and Schneider, R. W. 1991. Vegetative compatibility groups in Fusarium oxysporum f. sp. lycopersici. Phytopathology 91: 159-162. Fletcher, J. T., and Kingham, H. G. 1996. Fusarium wilt of cucumbers in England. Plant Pathol. 15: 85-89. Gaber, R. C., and Yoder, O. C. 1983. Isolational of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal, and plasmid components. Anal. Biochem. 135: 416-422. Garrett, R. H., and Amy, N. K. 1978. Nitrate assimilation in Fungi. Adv. Microb. Physiol. 18: 1-65. Gordon, T. R., and Okamoto, D. 1992. Variation in mitochondrial DNA among vegetatively compatible isolates of Fusarium oxysporum. Experimental Mycology 16: 245-250. Gordon, T. R., and Martyn, R. D. 1997. The evolutionary biology of Fusarium oxysporum. Annu. Rev. Phytopathology 35: 111-128. Grajal-Martin, M. J., Simon, C. J., and Muehlbauer, F. J. 1993. Use of random amplified polymorphic DNA (RAPD) to characterize race 2 of Fusarium oxysporum f. sp. pisi. Phytopathology 83: 612-614. Hendrix, J. W., Ducharme, E. P., and Murakishi, H. 1948. Pathogenicity of single-spore isolates of the Fusarium wilt organisms of muskmelon and watermelon. Phytopathology 38: 13. (Abstract) Huang, Z. X., Zhao, J. H., and Liu, Z. L. 1988. Observation on spore germination and growth of cucumber wilt disease fungus and ultrastructural study on fungal colonization in host tissue. Acta Agriculturae Shanghai. 4: 29-36. Jarvis, W. R., and Shoemaker, R. A. 1978. Taxonomic status of Fusarium oxysporum causing foot and root rot of tomato. Phytopathology 68: 1679-1680. Katan, T. 1996. Vegetative-compatibility groups in populations of Fusarium oxysporum in Israel. Phytoparasitica 24: 2. (Abstract) Katan, T., and Primo P. D. 1999 Current status of vegetative compatibility groups in Fusarium oxysporum: supplement. Phytoparasitica 27: 273-277 Kelly, A., Alcala-Jimenez, A. R., Bainbridge, B. W., Heale, J. B., Perez-Artes, E., and Jimenez-Diaz, R. M. 1994. Use of genetic fingerprinting and random amplified polymorphic DNA to characterize pathotypes of Fusarium oxysporum f. sp. ciceris infecting chickpea. Phytopathology 84: 1293-1298. Kistler, H. C. 1997. Genetic diversity in the plant-pathogenic fungus Fusarium oxysporum. Phytopathology 87: 474-479. Latin, R. X., and Snell, S. J. 1986. Comparison of methods for inoculation of muskmelon with Fusarium oxysporum f. sp. melonis. Plant Dis. 70: 297-300. Leslie, J. F. 1993. Fungal vegetative compatibility. Annu. Rev. Phytopathol. 31: 127-150. Manicom, B. Q., Bar-Josph, M., Kotze, J. M.,and Becker, M. M. 1990. A restriction fragment length polymorphism probe relating vegetative compatibility groups and pathogenicity in Fusarium oxysporum f. sp. dianthi. Phytopathology 80: 336-339. Manulis, S., Kogan, N., Reuven, M., and Ben-Yephet, Y. 1994. Use of the RAPD technique for identification of Fusarium oxysporum f. sp. dianthi from carnation. Phytopathology 84: 98-101. Marzluf, G. A. 1981. Regulation of nitrogen metabolism and gene expression in fungi. Microbiol. Rev. 45: 437-461. McDonald, B. A. 1997. The population genetics of fungi: Tools and techniques. Phytopathology 84: 98-101. Mckeen, C. D. 1951. Fusarium wilt of muskmelons and watermelons in southwestern Ontario. Phytopathology 41: 26. (Abstract) McMillan, R. T. 1986. Cross pathogenicity studies with isolates of Fusarium oxysporum from either cucumber or watermelon pathogenic to both crop species. Ann. Appl. Biol. 109: 101-105. Moreno, A., Alférez, A., Avilés, M., Diánez, F., Blanco, R., Santos, M., and Tello, J. C. 2001. First report of Fusarium oxysporum f. sp. radicis-cucumerinum on cucumber in Spain. Plant Dis. 85: 1206. Nelson, P. E., Toussoun, T. A., and Marasas, W. F. O. 1983. Fusarium Species: An illustrated manual for identification. The Pennsylvania University Press, University Park and London. Nelson, H., Ouchi, S., Shiraishi, T., and Oku, H. 1992. Induced resistance to Fusarium wilt of tomato and cucumber: symptoms and pathogen proliferation. Ann. Phytopathol. Soc. Japan 58: 659-663. Owen, J. H. 1955. Fusarium wilt of cucumber. Phytopathology 45: 435-439. Owen, J. H. 1956. Cucumber wilt, caused by Fusarium oxysporum f. cucumerinum n. f. Phytopathology 46: 153-157. Phae, C. C., Shoda, M., Kita, N., Nakano, M., and Ushiyama, K. 1992. Biological control of crown and root and bacterial wilt of tomato by Bacillus subtilis NB22. Ann. Phytopathol. Soc. Japan 58: 329-339. Puhalla, J. E. 1985. Classification of strains of Fusarium oxysporum on the basis of vegetative compatibility. Can. J. Bot. 36: 179-183. Puhalla, J. E., and Hummel, M. 1983. Vegetative compatibility groups within Verticillium dahliae. Phytopathology 73: 1305-1308. Punja, Z. K., and Parker, M. 2000. Development of Fusarium root and stem rot, a new disease on greenhouse cucumber in British Columbia, caused by Fusarium oxysporum f. sp. radicis-cucumerinum. Can. J. Plant Pathol. 22: 349-363. Rowe, R. C. 1995. Recent progress in understanding relationships between Verticillium species and subspecific groups. Phytoparasitica 23: 31-38. Snyder, W. C., and Hansen, H. N. 1940. The species concept in Fusarium. Amer. J. Bot. 27: 64-67. Vakalounakis, D. J. 1993. Inheritance and genetic linkage of Fusarium wilt (Fusarium oxysporum f. sp. cucumerinum race 1) and scab (Cladosporium cucumerinum) resistance genes in cucumber (Cucumis sativus). Ann. Appl. Biol. 123: 359-365. Vakalounakis, D. J. 1995. Allelism of the Fcu-1 and Foc genes conferring resistance to Fusarium wilt in cucumber. Eur. J. Plant Pathol. 102: 855-858. Vakalounakis, D. J. 1996. Root and stem rot of cucumber caused by Fusarium oxysporum f. sp. radicis-cucumerinum f. sp. nov. Plant Dis. 80: 313-316. Vakalounakis, D. J., and Fragkiadakis, G. A. 1999. Genetic diversity of Fusarium oxysporum isolates from cucumber: Differentiation by pathogenicity, vegetative compatibility, and RAPD fingerprinting. Phytopathology 89: 161-168. Vakalounakis, D. J. and Chalkias, J. 2004. Survival of Fusarium oxysporum f. sp. radicis-cucumerinum in soil. Crop Protection 23: 817-873. Vakalounakis, D. J., Wang, Z., Fragkiadakis, G. A., Skaracis, G. N., and Li, D.-B. 2004. Characterization of Fusarium oxysporum isolates obtained from cucumber in China by pathogenicity, VCG, and RAPD. Plant Dis. 88: 645-649. Weimer, J. L. 1944. Some root rots and a foot rot of lupines in the southeastern part of the United States. Journal of Agricultural Research 68: 441-457. Welsh, J., and McClelland, M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213-7218. Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingay, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535. Woo, S. L., Zoina, A., Sorbo, G. D., Lorito, M., Nanni, B., Scala, F., and Noviello, C. 1996. Characterization of Fusarium oxysporum f. sp. phaseoli by pathogenic races, VCGs, RFLPs, and RAPD. Phytopathology 86: 966-973. Wright, G. F. K., Guest, D. I., Wimalajeewa, D. L. S., and van Heeswijck, R. 1996. Characterisation of Fusarium oxysporum isolated from carnation in Australia based on pathogenicity, vegetative compatibility and random amplified polymorphic DNA (RAPD) assay. Eur. J. of Plant Pathol. 102: 451-457. Yildiz, M., and Delen, N. 1977. Studies on the occurrence of Fusarium wilt of cucumber Ege region of Turkey. J. Turk. Phytopathology 6: 111-117. Yoon, C. S., Glawe, D. A., and Shaw, P. D. 1991. A method for rapid small scale preparation of fungal DNA. Mycologia 83: 835-838. Zitter, T. A., Hopkins, D. L., and Thomas, C. E. eds 1996. Compendium of Cucurbit Diseases. APS, Minnesota, USA.
摘要: 
危害胡瓜的尖鐮胞菌(Fusarium oxysporum)有兩個生理分化型,分別是引起萎凋病的F. oxysporum f. sp. cucumerinum(F.o.c.)和引起根腐及莖腐病的F. oxysporum f. sp. radicis-cucumerinum(F.o.r.c.)。F.o.c.與F.o.r.c.之區別主要乃基於病徵、寄主範圍、最適發病溫度和營養體親和群(vegetative compatibility group, VCG)的不同,以及可用逢機增幅多形性DNA(random amplification of polymorphic DNA, RAPD)技術加以區別。在台灣,筆者於2003-2005年的田間病害調查中、南部的胡瓜田,發現罹患萎凋病的罹病株有的萎凋,有的表現根腐與莖腐的病徵。收集胡瓜產之尖鐮胞菌10個菌株加上購自美國菌種保存中心(American Type Culture Collection, ATCC)之F.o.c.標準菌株(ATCC 16416)以及12個F.o.c.與F.o.r.c.之VCG標準菌株來進行接種試驗,比較他們引起的病徵、寄主範圍與最適發病溫度,並測定VCG與RAPD等特性。結果顯示利用土壤混菌法接種胡瓜苗「萬吉」,除ATCC 16416外,其餘台灣產菌株均會造成根腐與莖腐的病徵。台灣產2個菌株(Foc-150與Foc-151)可感染胡瓜、洋香瓜、香瓜與西瓜,但ATCC 16416只能感染前三者,而不會感染西瓜。台灣產3個菌株(Foc-100、Foc-183與Foc-829)在17和28℃條件下均會使胡瓜發病,其餘台灣產供試菌株及ATCC 16416均在28℃為最適發病溫度。又在VCG的測試顯示,台灣產只有1個菌株(Foc-100)是屬於F.o.c.中VCG 0180的一群,其餘供試菌株則尚無法鑑定。利用UBC438、UBC489與UBC890三個引子作RAPD分析,結果顯示台灣產供試菌株與ATCC 16416及F.o.r.c.之VCG標準菌株(ATCC 204369與ATCC 204371)間的相似度僅56%,然而從引子UBC489和UBC890所增幅的RAPD指紋型來看,有4個菌株(Foc-100、Foc-151、Foc-183及Foc-106)與ATCC 16416類似。從上述接種病徵、寄主範圍、最適發病溫度、VCG及RAPD的測試結果指出,台灣產胡瓜萎凋病菌仍鑑定為Fusarium oxysporum f. sp. cucumerinum。

Two forma specialis (f. sp.) of Fusarium oxysporum were reported to be able to infect cucumber. One, which was Fusarium oxysporum f. sp. cucumerinum, caused Fusarium wilt of cucumber, and the other, F. oxysporum f. sp. radicis-cucumerinum (F.o.r.c.), caused root and stem rot of cucumber. Fusarium oxysporum f. sp. radicis-cucumerinum could be distinguished from F.o.c. with symptoms, host range, optimum infection temperature and vegetative compatibility group (VCG). Also, some scholars used random amplification of polymorphic DNA (RAPD) assay to distinguish these two forma specialis. In field of central and southern Taiwan, we found some of the diseased cucumber plants expressed wilt and some showed root and stem rot from 2003 to 2005. Ten pathogenic isolates of F. oxysporum which we collected from diseased cucumber were compared with the type strain of F.o.c. (ATCC 16416) and 12 VCG testers of F.o.c. and F.o.r.c. obtained from American Type Culture Collection (ATCC) according to symptoms, host rang, optimum infection temperature, VCG and RAPD fingerprinting. All Taiwan isolates could cause root and stem rot of cucumber plants (Vantage) by using soil infestation inoculation method, but ATCC 16416 did not. Two Taiwan isolates Foc-150 and Foc-151 could infect cucumber, muskmelon, melon and watermelon, but isolate ATCC 16416 could only infect cucumber, muskmelon and melon, not watermelon. Although 3 Taiwan isolates Foc-100, Foc-183 and Foc-829 could infect cucumber at both 17 and 28℃, the optimum infection temperature of other Taiwan isolates and isolate ATCC 16416 was 28℃. Vegetative compatibility tests indicated that 100M16, the nit mutant of isolate Foc-100 was VCG 0180 of F.o.c. The similarity ratios of RAPD of Taiwan isolates compared with ATCC 16416 and VCG testers of F.o.r.c. were only 56% by using primer UBC438, UBC489 and UBC890. But RAPD fingerprinting of 4 isolates Foc-100, Foc-151, Foc-183 and Foc106 by using primers UBC489 and UBC890 were similar to isolate ATCC 16416. Based on the results of symptoms, host range, optimum infection temperature, VCG and RAPD indicated that the Fusarium wilt pathogen of cucumber obtained from Taiwan was still identified as Fusarium oxysporum f. sp. cucumerinum.
URI: http://hdl.handle.net/11455/30949
其他識別: U0005-2707200617330000
Appears in Collections:植物病理學系

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.