Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31164
標題: 番茄內生性拮抗細菌防治番茄青枯病之研究
Studies on control of tomato bacterial wilt by endophytic antagonistic bacteria from tomato
作者: 陳美惠
Chen, Mei-huei
關鍵字: tomato;番茄;endophytic antagonistic bacteria;control;tomato bacterial wilt;內生性拮抗細菌;防治;番茄青枯病
出版社: 植物病理學系
摘要: 
由健康番茄植株莖部內分離到於培養基上對青枯病菌之生長具抑制作用的
拮抗細菌(208個菌株)及其他來源(青蔥根圈、菸草植株)拮抗細菌共227個
菌株,以切斷胚軸浸液法在網室測定對番茄青枯病之防治效果,結果僅
有9個菌株有顯著防治效果,其中以K8-1、EA2-4、KA4-1及A10-5四個菌株
在重複試驗中防治表現較穩定。這四個菌株都由健康番茄莖內分離而得,
本文稱之為內生性拮抗細菌。以Biology測定法鑑定,K8-1菌株屬於
Bacillus sp.;KA4-1及A10-5菌株屬於Pseudomonas spp.,而EA2-4菌株
則尚不能判定其屬名。內生性拮抗細菌以切胚軸浸液法防治番茄青枯病之
效果受到一些因子之影響,而這些影響因拮抗菌株而異。拮抗細菌於番茄
株齡10及13天大時處理,較能表現防治效果;浸液拮抗細菌的時間過長或
過短,較不易達到防治效果,最好的浸液時間為12小時;在抗感病品種或
品系上測試防治效果時,除EA2-4菌株外,都能顯現其防治效果;拮抗細
菌的施用濃度以108cfu/ml的防治效果較佳;拮抗細菌對青枯病菌不同菌
株引起的青枯病之防治效果有差異;土壤中青枯病菌的濃度為107或106
cfu/g dry soil時,拮抗細菌較易表現對青枯病的防治效果;利用不同方
式處理拮抗細菌時,以切斷胚軸浸液接種方式及種子被覆法較能達到防治
病害之目的;溫度亦會影響拮抗細菌防治青枯病之表現,一般在溫度為30
℃時,最能表現其防治效果。拮抗細菌以切胚軸浸液輸入番茄植株體內後
,植株根部及莖部均有拮抗細菌之存在,各拮抗細菌數目皆隨番茄生長期
的增長而緩慢遞減,其中莖部族群量的下降速度較快;拮抗細菌輸入植株
體內42天後,各菌株根部族群量約為104-106cfu/g fresh root,莖部族
群量約為103-105cfu/g fresh stem,其中在根部及莖部以KA4-1菌株族群
量為最高,分別達1.17×106cfu/g fresh root及2.90×105cfu/g fresh
stem,最低者為EA2-4菌株分別為1.80×104cfu/g fresh root及1.35
×103cfu/g fresh stem。拮抗細菌與青枯病菌在番茄植株體內共存競爭
時,拮抗細菌在根部及莖部的族群量變化與上述者相似,而青枯病菌的族
群量則隨時間的增長逐漸遞增。雖然青枯病菌之族群看似無明顯下降趨勢
, 但拮抗細菌處理的番茄植株其發病程度均顯著低於未處理的對照植株

A total of 227 bacterial strains antagonistic to Pseudomonas
solanacearum on media were isolated from interior tissues of
stems of healthy tomato plants ( 208 strains ) and other sources
(welsh onion rhizospheres , tobacco plants ). They were tested
for the ability to control tomato bacteria wilt after
introducing into plants by the Kijima,s hypocotyl-cuttings
method in a screenhouse. Only nine strains can reduce the
disease severity significantly. Among the nine strains, strains
K8-1、EA2-4、KA4-1 and A10-5 were more consistent in their
control efficacy during repeated testing. These four strains
were all isolated internaly from the stem of healthy tomato
plants, and were termed endophytic antagonistic bacteria in this
study. Strain KA8-1 was identified as Bacillus sp., strains
KA4-1 and A10-5 as Pseudomonas spp. , but strain EA2-4 could not
be identified based on the Biolog identification system. Factors
affecting the biocontrol efficacy of the four strains of
endophytic antagonistic bacteria by the hypocotyl-cutting method
were studied in the greenhouse. The influence of each factor on
the control efficacy varied among tested antagonistic bacteria.
The antagonistic bacteria were more effective when using 10 and
13-day old seedlings for hypocotyl-cutting treatment than
seedlings at the other ages. When soaking period during the
hypocotyl-cuttings treatment was too long or short, it was not
suitable for all or some strains of the antagonistic bacteria ;
the best soaking period was 12 hr. Except strain EA2-4,
allstrains showed biocontrol efficacy on susceptible and
resistant varieties ( lines ) of tomato. The best concentration
of the antagonistic bacteria used for dipping was 108 cfu/ml .
Control efficacy of the antagonistic bacteria varied when the
disease was caused by different strains of P. solanacearum. The
control efficacy of antagonistic bacteria was more easily
demonstrable in the soil infested with P. solanacearum at the
density of 107 or 106 cfu/g dry soil. Biocontrol efficacy of the
antagonistic bacteriawas affected by temperature ; Except strain
EA2-4, the best control efficacy was obtained at 30℃. Among
different treatment methods of antagonistic bacteria , the
hypocotyl-cutting and seed bacterization methods resulted the
best control efficacy by all or most strains of the antagonistic
bacteria. The antagonistic bacteria existed in stems and roots
after introducing into tomato plants by the hypocotyl-cutting
method. The populations of antagonistic bacteria declined more
slowly in roots than in stems with the time during the growth of
tomato. Fourty-two days after introduced into plants,
populations of all antagonistic strains in roots and stems
remained as 104-106 cfu/g fresh root and 103-105 cfu/g fresh
stem, respectively. The population of K8-1 was the highest
detected in the plant, which were 1.17×106 cfu/g fresh root and
2.90×105 cfu/g fresh stem, respectively; the population of
EA2-4 was the lowest, which were 1.80×104 cfu/g fresh root and
1.35×103 cfu/g fresh stem, respectively.When the antagonistic
bacteria and P. solanacearum were present together in the tomato
plants, the population dynamics of the antagonistic bacteria in
roots and stems were similar to those described above, whereas
the populations of P. solanacearum increased with the time. Even
though the population of P. solanacearum did not seem to be
declined, the disease severities of the plants treated with all
antagonistic bacteria were significantly lower than control
plants.
URI: http://hdl.handle.net/11455/31164
Appears in Collections:植物病理學系

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