Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31202
標題: 絲瓜萎凋病菌之生理小種分化及嫁接抗病根砧之防治
Race Differential of Fusarium oxysporum f. sp. luffae and its Control by Using Resistant Rootstocks
作者: 張家瑋
Chang, Chia-Wei
關鍵字: loofah;絲瓜;Fusarium wilt;race;Fusarium oxysporum;graft;萎凋病;生理小種;嫁接
出版社: 植物病理學系所
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摘要: 
本論文之研究目的在探討台灣絲瓜萎凋病菌 (Fusarium oxysporum f. sp. luffae) 是否具生理小種分化,以及發展利用抗病圓筒型絲瓜作為根砧,以 1:3 (1株接穗同時嫁接 3 株根砧) 割裂靠接法來防治絲瓜萎凋病。目前台灣地區之絲瓜萎凋病菌,依據在PDA斜面培養基上的色素特徵可區分為二群,其中產生紫黑色素之菌株主要分離自台中市軍功寮、南投縣魚池鄉、高雄縣旗山鎮及大樹鄉等地;產生淡橙色素者則在埔里鎮水蛙窟以及大坪頂地區分離得到。將產生紫黑色素之代表菌株 Fol-638 與產生淡橙色素之代表菌株 Fol-662作比較,發現在各種孢子之型態大小以及菌絲最適生長溫度上差異不顯著。但在混菌土壤接種試驗發現,屬「綠皮種」絲瓜之「東光」品種,接種 Fol-638造成之發勢較Fol-662 嚴重 (發病度分別為 53 和 8%);然而屬「白皮種」絲瓜之「銀光」品種接種後出現之發勢則差異不顯著 (發病度介於為 78-83%)。故在本研究中利用絲瓜「東光」和「銀光」作鑑別品種,可將台灣之絲瓜萎凋病菌區分成兩個生理小種:第一生理小種僅可危害「銀光」,而第二生理小種則可造成此二品種發病。在菌株毒力與色素關係上,將屬於淡橙色素之Fol-167 供試菌株以混土接種至「東光」上,與接種 Fol-638 之結果差異不顯著 (發病度為 42 和 53%),故菌株之色素特徵與對寄主之毒力不相關。而在田間之嫁接防治試驗發現,選用較為抗病之圓筒型絲瓜品種/系作為根砧,利用割裂靠接法嫁接多株根砧,並及時剪除接穗地基部可增進嫁接絲瓜對萎凋病之防治效果。

In this thesis, two subjects were studied: to confer the existence of races of Fusarium oxysporum f. sp. luffae in Taiwan and management of Fusarium wilt of loofah by using 1:3 cleft inarching with resistant cylindrical loofah as rootstock. Fusarium oxysporum f. sp. luffae in Taiwan could be grouped into two types according to their pigmentation on potato dextrose agar medium (PDA): dark purple type (represented by Fol-638) was isolated from Jyungong, Yuchih, Chishan and Dasu and light orange type (represented by Fol-662) was isolated from Puli and Dapingding. There was no difference between Fol-638 and Fol-662 on spores shape, size and the most suitable temperature of the mycelial growth. Disease severity on the cultivar "Cylinder" inoculated with Fol-638 was higher ( 53 and 8 % ) than it inoculated with Fol-662 by soil infestation inoculation method; nevertheless, it was similar ( 78 - 83 % ) that disease severity on the cultivar "Shimmery" inoculated both test isolates by the same method. According to above-listed tests, Fusarium oxysporum f. sp. luffae could be separated into two races: race 1 attacked "Shimmery" only, but race attacked both "Shimmery" and "Cylinder". Besides, the virulence types was not corresponding to the pigmentation types of this fungi in that the cultivar "Cylinder" showed symptoms when it inoculated with the light orange type isolate Fol-167 or the dark purple type isolate Fol-638 by soil infestation inoculation method. Field grafting control experiment showed that the disease will be controlled more by using one scion grafts with numbers of resistant cylindrical loofahs as rootstocks and pruning the basal part of scion in time.
URI: http://hdl.handle.net/11455/31202
其他識別: U0005-1102200915125700
Appears in Collections:植物病理學系

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