Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31315
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dc.contributor謝廷芳zh_TW
dc.contributorTing-Fang Hsiehen_US
dc.contributor張碧芳zh_TW
dc.contributorPi-Fang Linda Changen_US
dc.contributor.advisor黃振文zh_TW
dc.contributor.advisorJenn-Wen Huangen_US
dc.contributor.author陳睦鈞zh_TW
dc.contributor.authorChen, Mu-Chunen_US
dc.contributor.other中興大學zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-06T07:41:29Z-
dc.date.available2014-06-06T07:41:29Z-
dc.identifierU0005-1808200911210100zh_TW
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Phosphate solubilizing bacteria and their roles in plant growth promotion. Biotechnol. Adv. 17:319-339. Ilbas, A. I. and Sahin, S. 2005. Glomus fasciculatum inoculation improves soybean production. Acta Agr. Scand. B-S. P. 55: 287-292. Jacobsen, B. J., Zidack, N. K. and Larson, B. J. 2004. The role of Bacillus-based biological control agents in integrated pest management systems. Phytopathology 94:1272-1275. John, M. B., Richard, M. B. and Sara, E. S. 1991. Rapid in situ assay for indole-acetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl. Environ. Microbiol., 57(2): 535-538. Jose, L., Juan, C. C., Erasto, H., Crisanto, V., Rodolfo, F., Lourdes, I. M. and Eurduardo, V. 2007. Bacillus megaterium rhizobacteria promote growth and alter root-system architecture through an auxin- and ethylene-independent signaling mechanism in Arabidopsis thaliana. Mol. Plant. Microbe. Inter. 20(2):207-217. Kenerley, C. M., Bruck, R. I. and Grand, L. F.. 1984. 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Microbiol. 64:3860-3868. Solon, A. G. and Robert, P. W. 1951. Colorimetric Estimation Of Indole-acetic Acid. Plant Physiol. 26:192-195. Song, O., Lee1, S. J., Lee1,Y. S., Lee1, S. C., Kim, K. K. and Choi, Y. L. 2008. Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil. Braz. J. Microbiol. 39:151-156. Tejera, N., Lluch, C., Mart’ınez-Toledo, M.V. and Gonz’alez-L’opez, J. 2005. Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere. Plant Soil 270:223-232. Tetsu, H. 1981. Properties of Amino Acid Composition of the Tryptic Fragments of the Heavy Chain of Myosin Subfragment. J. Biochem. 90:785-788. Vivek, K., Rishi, K. B. and Neeru, N. 2001. Establishment of phosphate-solubilizing strains of Azotobacter chroococcum in the rhizosphere and their effect on wheat cultivars under green house conditions. Microbiol. Res. 156:87–93. Wadii, B., Joan, P., Francisco, R. T., Manuel, T. and Eduardo, P. 1997. Pollination increases gibberellin levels in developing ovaries of seeded varieties of citrus. Plant Physiol. 114:557-564. Young, C. C. 1990. Effects of phosphorus-solubilizing bacteria and vesicular-arbuscular mycorrhizal fungi on the growth of tree species in subtropical-tropical soils. Soil Sci. Plant Nutr. 36:225-231. Youssef, Y. A. and Mankarios, A. T. 1974. Production of plant growth substances by rhizosphere mycoflora of broad bean and cotton. Biol. Plantarum 17(3):175-181. Zhen M., Li, G., Anna, S.Y., Leea, J., Wan, H. Y., Swee, N. T. and Eng, S. O.. 2008. Simultaneous analysis of different classes of phytohormones in coconut (Cocos nucifera L.) water using high-performance liquid chromatography and liquid chromatography-tandem mass spectrometry after solid-phase extraction. Anal. Chim. Acta 610:274–281.zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/31315-
dc.description.abstract測試72株細菌產生吲哚乙酸(Indole-3-acetic acid, IAA)的能力,結果發現Gh1與Gh4菌株產生IAA的濃度最高,約61~80 mg/L;其次有Bg12、BSSC-01、Bg31及Bg26等15菌株之產量介於10~60 mg/L。經鑑定Gh1、Gh4、Bg12及Bg31等菌株的學名,分別是Paenibacillus polymyxa、Geobacillus thermoblucosidasius、Bacillus megaterium及Microbacterium resistense。色胺酸 (L-form Tryptophan)是細菌產生IAA的決定性因子。本研究在LB (Luria-Bertani)培養液中添加不同濃度的色胺酸,結果顯示隨著色胺酸濃度的提高,IAA產量也隨著增加。測試不同的培養基質對菌量增殖及產生IAA的影響,發現大豆酪蛋白培養液(Trypic soy broth;TSB)優於其餘培養液。將番茄、甜瓜及蘿蔔種子分別浸泡於上述菌株之細胞懸浮液,並種植於栽培基質中,結果發現Gh1、Gh4及Bg31等三菌株可顯著促進甜瓜及番茄幼苗的生長。另外將四菌株分別培養於TSB後,混拌於介質後並種植不同作物,結果發現菌株Bg31促進蘿蔔生長之效果最佳。將蘿蔔幼苗培養在(Murashige and Skoog medium, MS)植物組織培養液中,分別加入Bg31菌株之TSB培養液與IAA標準品,評估它們對幼苗根系的發育的影響,結果發現兩處理均可促進支根的發育且誘使支根較為粗壯。進一步,將Bg31菌株之花生粕醱酵培養液與味丹公司的糖蜜醱酵液混合施用,證明可顯著促進蘿蔔植株鮮重增加一倍以上。zh_TW
dc.description.abstractSeventy two bacterial isolates were examined the ability of production Indole-3-acetic acid (IAA). Among them, Gh1 and Gh4 isolates produced the highest concentration of IAA (61~80 mg/L), followed by Bg12 and Bg31 isolates. These isolates (Gh1, Gh4, Bg12 and Bg31) were identified as Paenibacillus polymyxa, Geobacillus thermoblucosidasius, Bacillus megaterium and Microbacterium resistense ,respectively. The L-form tryptophan was a determinant factor for these isolates to produce IAA. The production of IAA was increased, with the increase of tryptophan in LB (Luria-Bertani) broth. The ability of producing IAA by bacterial isolates grown in different commercial media was tested. The tryptic soy broth (TSB) showed the best effect for these isolates to produce IAA, and increased the biomass of bacterial isolates. The seeds of tomato, melon and radish were dipped into bacterial cell suspension and sown in peat moss. The results indicated that Bg31, Gh1 and Gh4 isolates could significantly promote the seedlings growth of melon and tomato. The Bg31 isolate showed marked effect on promoting the growth of radish seedlings when its culture filtrate of TSB was added into peat moss before sowing. In addition, the Bg31 isolate cultured in TSB showed similar effect on promoting branch root development of radish seedlings as treatment with IAA standard. For practical use, a formula consisted of molasses and peanut seed pomace meal was used for culturing Bg31 isolate and showed significant effect on promoting the growth of radish plants.en_US
dc.description.tableofcontents中文摘要........................................................................................................................I 英文摘要.......................................................................................................................II 目錄.............................................................................................................................IV 表目錄........................................................................................................................VII 圖目錄...................................................................................................................... VIII 前言................................................................................................................................1 材料與方法....................................................................................................................7 一、供試植株..................................................................................................................7 二、吲哚乙酸的濃度標準曲線製作與檢測..................................................................7 三、篩選具有產生吲哚乙酸能力之菌株測試平台......................................................7 四、具有產生吲哚乙酸能力菌株的分離及篩選..........................................................8 (一). 菌株分離........................................................................................................8 (二). 具有產生吲哚乙酸能力之菌株篩選............................................................8 (三). 菌株產生吲哚乙酸與吲哚乙酸標準品之比較............................................8 五、菌株鑑定 (一). 脂肪酸鑑定系統............................................................................................9 (二). Biolog快速偵測系統...................................................................................10 (三). 16S rRNA 的鑑定.......................................................................................10 六、影響菌株產生吲哚乙酸濃度之因子探討............................................................11 (一). 不同色胺酸濃度對菌株產生吲哚乙酸的影響..........................................11 (二). 光照對Gh1、Gh4、Bg12、Bg31菌株產生吲哚乙酸的影響........................12 (三). 培養時間對Gh1、Gh4、Bg12、Bg31菌株產生吲哚乙酸的影響................12 (四). 溫度對Gh1、Gh4、Bg12、Bg31菌株產生吲哚乙酸的影響........................12 (五). 酸鹼值對Gh1、Gh4、Bg12、Bg31菌株產生吲哚乙酸的影響....................13 (六). 接種菌量濃度對Gh1、Gh4、Bg12、Bg31菌株產生吲哚乙酸的影響........13 (七). 氮素源對Gh1、Gh4、Bg12及Bg31菌株菌量增殖及產生吲哚乙酸的影響..................................................................................................................13 (八). 碳素源對Gh1、Gh4、Bg12及Bg31菌株菌量增殖及產生吲哚乙酸的影響..................................................................................................................14 七、不同培養基質影響Gh1、Gh4、Bg12、Bg31菌株產生吲哚乙酸之探討..............15 八、四菌株之細胞懸浮液對作物生長的影響...........................................................15 九、四菌株之培養液對作物生長的影響....................................................................15 十、菌株培養液內IAA濃度對植物根系生長之影響...............................................16 十一、修正培養基配方對Bg31菌株菌量增殖及產生吲哚乙酸的影響..................16 十二、不同有機添加物對Bg31菌株菌量增殖及產生吲哚乙酸的影響..................17 十三、Bg31菌株之醱酵液對作物生長的影響結果.................................................18 十四、Bg31菌株之醱酵液及微量元素營養液對作物生長的影響..........................18 結果…………………………………………………………………………………..19 一、具有產生吲哚乙酸能力菌株的分離及篩選.......................................................19 二、菌株鑑定...............................................................................................................19 (一). 脂肪酸鑑定系統..........................................................................................19 (二). Biolog快速偵測系統...................................................................................20 (三). 16S rRNA 之鑑定.......................................................................................20 三、影響菌株產生吲哚乙酸濃度之因子探討...........................................................20 (一). 不同色胺酸濃度對菌株產生吲哚乙酸的影響..........................................20 (二). 光照對Gh1、Gh4、Bg12及Bg31菌株產生吲哚乙酸的影響.....................21 (三). 培養時間對Gh1、Gh4、Bg12及Bg31菌株產生吲哚乙酸的影響.............21 (四). 溫度對Gh1、Gh4、Bg12及Bg31菌株產生吲哚乙酸的影響...............21 (五). 酸鹼值對Gh1、Gh4、Bg12及Bg31菌株產生吲哚乙酸的影響.................22 (六). 接種菌量濃度對Gh1、Gh4、Bg12及Bg31菌株產生吲哚乙酸的影響.....22 (七). 氮素源對Gh1、Gh4、Bg12及Bg31菌株菌量增殖及產生吲哚乙酸的影響..................................................................................................................22 (八). 碳素源對Gh1、Gh4、Bg12及Bg31菌株菌量增殖及產生吲哚乙酸的影響..................................................................................................................23 四、不同培養基質對Gh1、Gh4、Bg12及Bg31菌株菌量增殖及產生吲哚乙酸的 影響..........................................................................................................................24 五、四菌株之細胞懸浮液對作物生長的影響...........................................................24 六、四菌株之培養液對作物生長的影響...................................................................25 七、菌株培養液內IAA濃度對植物根系生長之影響.............................................25 八、修正培養基配方對Bg31菌株菌量增殖及產生吲哚乙酸的影響..................... 25 九、不同有機添加物對Bg31菌株菌量增殖及產生吲哚乙酸的影響.....................26 十、Bg31之醱酵液對作物生長的影響.....................................................................26 十一、Bg31菌株之醱酵液與微量元素營養液對作物生長的影響..........................26 討論…………………………………………………………..………………………28 引用文獻………………………………………………..……………………………32 圖表………………………………………………..…………………………………38 附錄………………………………………………..…………………………………66zh_TW
dc.language.isoen_USzh_TW
dc.publisher植物病理學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1808200911210100en_US
dc.subjectrhizosphere bacteriaen_US
dc.subject吲哚乙酸zh_TW
dc.subjectindole-3-acetic aciden_US
dc.subjecttryptic soy brothen_US
dc.subjectradishen_US
dc.subjectpeanut seed pomaceen_US
dc.subject大豆酪蛋白培養液zh_TW
dc.subject蘿蔔zh_TW
dc.subject花生粕zh_TW
dc.title利用根圈細菌研製生物性肥料的評估zh_TW
dc.titleEvaluation for development of a biofertilizer using rhizobacteriaen_US
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
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