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標題: 許旺細胞與幹細胞共培養殖入神經導管應用於坐骨神經修復
Sciatic Nerve Regeneration by Co-Cultured Schwann Cells and Stem Cells on Microporous Nerve Conduits
作者: 戴念國
Dai, Lien-Guo
關鍵字: 許旺細胞;Schwann cells;脂肪幹細胞;牙髓幹細胞;周邊神經損傷;神經再生;神經導管;adipose-derived adult stem cells;dental pulp stem cells;peripheral nerve injury;nerve regeneration;conduits
出版社: 化學工程學系所
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此研究中,不同型態之同物種細胞包含許旺細胞、脂肪幹細胞、牙髓幹細胞以及許旺細胞與脂肪幹細胞或牙髓幹細胞之共培養,移植進入神經導管來修復大鼠坐骨神經大間距(15 mm)之神經缺損。並經由組織切片染色、電生理、步跡與步態分析來評估導管植入8週期間與之後其神經再生能力與功能性恢復情形。同時體外細胞培養實驗來瞭解神經滋養因子(如神經生長因子、腦衍生神經滋養因子、膠細胞衍生神經滋養因子)之協同作用。

Cell transplantation is a useful therapy for treating peripheral nerve injuries. The clinical use of Schwann cells (SCs), however, is limited because of their limited availability. An emerging solution to promote nerve regeneration is to apply injured nerves with stem cells derived from various tissues. In this study, different types of allogenic cells including SCs, adipose-derived adult stem cells (ASCs), dental pulp stem cells (DPSCs) and the combination of SCs with ASCs or DPSCs were seeded on nerve conduits to test their efficacy in repairing a 15 mm-long critical gap defect of rat sciatic nerve. The regeneration capacity and functional recovery were evaluated by the histological staining, electrophysiology, walking track and functional gait analysis after 8 weeks of implantation. An in vitro study was also performed to verify if the combination of cells led to synergistic neurotrophic effects (NGF, BDNF and GDNF). Experimental rats receiving conduits seeded with a combination of SCs and ASCs had the greatest functional recovery, as evaluated by the walking track, functional gait, nerve conduction velocity (NCV) and histological analysis. Conduits seeded with cells were always superior to the blank conduits without cells. Regarding NCV and the number of blood vessels, conduits seeded with SCs and DPSCs exhibited better values than those seeded with DPSCs only. Results from the in vitro study confirmed the synergistic NGF production from the co-culture of SCs and ASCs. It was concluded that co-culture of SCs with ASCs or DPSCs in a conduit promoted peripheral nerve regeneration over a critical gap defect.
其他識別: U0005-3110201210590900
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