Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31419
標題: Fusarium solani及F. ventricosum菌株間可溶性蛋白與同功異構酵素電 泳圖譜之比較
Comparison the soluble protein and isozyme electrophoresis patterns between Fusarium solani and F. ventricosum
作者: 曾春僑
Tzeng, chun-chiao
關鍵字: Fusarium;鐮孢菌;protein;isozyme;蛋白質;同功構酵素
出版社: 植物病理學系
摘要: 
Fusarium 屬真菌之分類主要是依據其胞子叢、大胞子及小胞子的形態而
來,然而, 其中卻存在著許多極小之差異,以致於在較細微之鑑定上難
以達到理想之效果。近幾年來,為解決形態判定上之難題,利用真菌細胞
體內抽取之各種物質進行電泳,比較彼此間之差異性的方法,已廣泛的被
應用於一些較具爭議性之真菌分類問題上。 在 Fusarium 屬中,F.
sloani及 F. ventricosum 之形態構造極為相似,兩者除產胞方式有些許
不同外,其餘並無大之差異性存在,因此,這兩種真菌是否應分開或合併
成一種目前仍具許多之爭議性。本實驗利用聚丙烯醯胺凝膠電泳比較其電
泳圖譜之差異以及透過 UPGMA 分析法比較兩者間之相似係數。在所有之
分離株中均可發現到三條主要蛋白條帶,80, 60 及 50 kd 之存在;除此
之外,所有之分離株不論在條帶之數目及濃度上均有所差異。在孔雀藍染
色以及銀染上相似度最高值分別為 0.97 及 0.94,最低值分別為 0.59
及 0.56。在二十二種受測試的同功異構酵素中,十六種之結果完全相同
,惟其中的十五種出現不同濃度之差異。所測試到的二十個等位基因中有
十四個完全相同;在反應相異的基因座上分別可觀察到一至三個的等位基
因之存在。 在一些分離株的核酸電泳中,可發現到質體 DNA 之存在。在
所測試的限制酵素中並無法找到一個適合所有菌株之內切酵素以作為限制
片段圖譜分析用。由我們所得知結果看來,此兩種真菌間存在著相當程度
之相似性,因此建議將兩種真菌併稱為 F. solani。
Taxonomy of the genus Fusarium is based primarily on
morphology of theirsporodochia, microconidia and macroconidia,
however, many of their morphologyis hard to identify because
of the small differences between these species.Recently, gel
electrophoresis extracted from various fungal components
hasreceived considerable attention as a possible aid in
resolving some of theproblems inherent in fungal taxonomy. Two
Fusarium species, F. solani and F.ventricosum, their
morphological structure were correspondly only in
thedifferences of conidiophore structure. Some arguments were
raised whether thetwo species are combined or not. These two
Fusarium species were compared byusing SDS-polyacrylamide gel
electrophoresis of total soluble proteins and thesimilarity
coefficients were obtained through the unweight pair-group
methodwith arithmetic average (UPGMA). Three major bands,
80, 60 and 50 kd, wereobserved in all the isolates. And the
differences of total soluble proteinswere not only in their
concentration but also in the location that appeared inthe gel.
The highest similarity value within the Coomassie blue
stainingcluster was 0.97 and silver slat staining cluster
was 0.94. The lowestsimilarity values within the Coomassie
blue stain cluster was 0.59 and silversalt stain cluster 0.56.
The activity of twenty-two enzymes were screened inoptimum
buffer system. The electrophomorphs of all isolates were the
same insixteen isozyme reaction and fifteen enzymes were showed
strong activity andacceptable resolution. Fourteen of the
20 alleles exhibited completehomogeneity among all isolates
surveyed and six of the alleles were variableand revealed one
to three putative alleles per locus. The plasmid were existedin
some isolates and non of suitable restriction enzyme were
found to digestthe DNA of these isolates for restriction
fragment length polymorphisms. Theresults suggested that
these isolates of the two Fusarium species maybecombined
together and named as F. solani due to their high degree
ofsimilarity in soluble proteins and isozymes.
URI: http://hdl.handle.net/11455/31419
Appears in Collections:植物病理學系

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