Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/31453
標題: 自蝴蝶蘭分離之葉芽線蟲Aphelenchoides bicaudatus與Aphelenchoides sp. 之鑑定與生態研究
The identification and ecology of Aphelenchoides bicaudatus and Aphelenchoides sp. isolated from Phalaenopsis
作者: 簡凡喻
Jain, Fan-Yu
關鍵字: Aphelenchoides;葉芽線蟲;identification of nematodes;phylogenetic analysis;Phalaenopsis;線蟲鑑定;形態發生學;蝴蝶蘭
出版社: 植物病理學系所
引用: 參考文獻 王貴美, 蔡東纂, 林奕耀. 1993. 台灣草莓葉芽線蟲病之發生及其生態研究. 植保會刊 35:14-29. 林奕耀, 王貴美, 蔡東纂. 1992. 秋石斛葉芽線蟲病之發生. 植保會刊 34:202-215. 楊宗勳. 2005. 自水蘊草 Hydrilla verticillata 分離之葉芽線蟲 Aphelenchoides sp. 之鑑定與生態研究. 國立中興大學植物病理研究所第三十五屆碩士論文 周國梁. 1992. 台灣蘭花植物寄生線蟲的鑑定. 中國植物病理學報 22: 234-239 游培琪. 2002. 山蘇花葉芽線蟲病害在台灣之發生. 國立中興大學植物病理研究所第三十二屆碩士論文 蔡東纂. 1999. 植物寄生性線蟲病害之化學防治. 植物病理學會刊 8:41-50. 蔡東纂. 1995. 植物病原線蟲檢疫. 植病會刊 4:43-59. 蔡東纂. 1998. 植物寄生性線蟲之鑑定與病害診斷. 種苗檢查技術訓練講義 (線蟲之鑑定):110-124. 蔡東纂, 程永雄. 1994. 台灣花卉線蟲問題. 台灣花卉病蟲害研討專刊 pp:225-241. 蔡東纂, 程永雄, 陳弘毅, 林奕耀, 吳文希. 1995. 球根花卉線蟲病害之發生及防治. 植物病理學會刊 4:180-192. 蔡雲鵬. 1971. 台灣之植物寄生性線蟲. 台灣農業 7(3):903-914 行政院農業委員會動植物防疫檢疫局. 2004. 台灣輸美附帶栽培介質植物工作計畫. 行政院農業委員會動植物防疫檢疫局. 2004. 輸美附帶介質蝴蝶蘭指定栽培溫室定期檢查表. 行政院農業委員會. 2007. 95年農業統計年報. Bos, W. S. 1977. Aphelenchoides arachidis n. sp. (nematoda: Aphelenchoidea), an endoparasite of the testa of groundnuts in Nigeria. Z. Pflanzenk. Pflanzenk. 84:95-99. B''Chir, M. M. 1979. Description and biometrical, morphological and biological studies of Aphelenchoides tuzeti n. sp. (nematoda: Aphelenchoidea) and discussion on the specific criteria applied to the genus Aphelenchoides. Nematologica 25:22-31. De Ley, I. T., De Ley, P., Vierstraete, A., Karssen, G., Moens, M., and Vanfleteren, J. 2002. Phylogenetic analyses of Meloidogyne small subunit rDNA. J. Nematol. 34(4):319-327. De Man, J. G. 1884. Die einheimischen, frei in der reinen Erde und im sussen Wsasser Iebenden Nematoden. Ned. Dierk. Ver. 5:1-104. Floyd, R. M., Rogers, A. D., Lambshead, P. J. D. and Smith, C. R. 2005. Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes, 5: 611–612. Franklin, M. T. 1957. Aphelenchoides composticola n. sp. and A. saprophilus n. sp. from mushroom compost and rotting plant tissues. Nematologica 2:306-313. Franklin, M. T. and Siddiqi, M. R. 1972. Aphelenchoides besseyi. In CIH Description of Plant-Parasitic Nematodes, Set 1, No. 4. Commonwealth Institute of Helminthology, St. Albans, England. Hooper, D. J. 1958. Aphelenchoides dactylocercus n. sp. and A. sacchari n. sp. (Nematoda: Aphelenchoidea). Nematologica 3, 229-235. Hunt, D.J. 1993. Aphelenchida, Longidoridae and Trichodoridae: Their Systematics and Bionomics. CAB International Wallingford (United Kingdom). Nickle, W. R. and Hooper, D. J. 1991. The Aphelenchina: Bud, Leaf, and Insect Nematodes. In Manual of Agricultural Nematology, W. R. Nickle, eds. Marcel Dekker, Inc., New York, USA, pp.465-507. Shahina, F. 1996. A Diagnostic compendium of the genus Aphelenchoides Fisher, 1894 (Nematoda: Aphelenchida) with some new records of the group from Pakistan. Pakistan Journal of Nematology 14:1-32. Siddiqui, I.A., and Taylor, D. 1969. Feeding mechanisms of Aphelenchoides bicaudatus on three fungi and an alga. Nematologica 15:503-509(7) Siddiqi, M. R. 1974. Aphelenchoides ritzemabosi. In CIH Description of Plant-Parasitic Nematodes, Set 3, No. 32. Commonwealth Institute of Helminthology, St. Albans, England. Siddiqi, M. R. 1974. Aphelenchoides fragariae. In CIH Description of Plant-Parasitic Nematodes, Set 5, No. 74. Commonwealth Institute of Helminthology, St. Albans, England. Siddiqi, I. A. 1976. Aphelenchoides bicaudatus. In CIH Description of Plant-Parasitic Nematodes, Set 6, No. 84. Commonwealth Institute of Helminthology, St. Albans, England. Thorne, G. 1962. Principles of nematology. McGraw Hill Brook Cp., New York 553 pp. Thorne, G. 1961. Principles of nematology. McGraw Hill Brook Co., New York 553 pp. Waele, D. D. 2002. Foliar Nematodes: Aphelenchoides Species. In: Plant Resistance to Parasitic Nematodes. R. Cook, J. Bridge, and J. L. Starr, eds. CAB International, Walingford, UK, pp.141-151.
摘要: 
本研究於2007年調查我國中南部48個蝴蝶蘭園內的葉芽線蟲分佈,其中可發現葉芽線蟲的蝴蝶蘭園高達35家,佔總調查蘭園的72%。於調查的過程中發現並收集了3種純系培養的葉芽線蟲族群ABC、AL、AU,其皆可於光學顯微鏡下觀察到典型的Aphelenchoides屬特徵,線蟲體形呈線形,具有口針及口針結球,食道部分部皆為3部食道,中部食道球寬度佔體寬的3/4以上,卵巢型態為單卵巢並具有後部子宮支囊,根據外部形態與第曼公式的測量可判定ABC為Aphelenchoides bicaudatus,而AU及AL按照其尾部與測線特徵推測與Aphelenchoides composticola最為相似。本實驗使用的18S rDNA引子對增幅並定序3種葉芽線蟲之18S rDNA,與Genebank資料庫比對後佐證ABC蟲系為Aphelenchoides bicaudatus,AU與AL為彼此親緣關係相當接近的線蟲,雖無法在Genebank內搜尋到可能為AU或AL的種類,但可初步排除為4大經濟危害葉芽線蟲的可能。3種葉芽線蟲族群(AU、AL、ABC)皆可以針刺接種1000隻線蟲的方式接種於蝴蝶蘭根部後,使用棉藍染色法觀察到在根部內的葉芽線蟲與蟲卵。3種葉芽線蟲除了可在人工接種的情況下侵染蝴蝶蘭,此外亦可人工接種於水稻與草莓並再次被分離到,此外其餘植物如石斛蘭、山蘇、非洲堇、仙客來等均無病徵且無法分離出原接種病原。在防治藥劑篩選及藥害測試實驗之後發現加保扶(Carbofuran)、芬滅松(Phenamiphos)及滅線蟲(DCIP)有較佳的防治效果且較不易產生藥害。

In this study, we surveyed the distribution of the foliar nematodes (Aphelenchoides) in 48 Phalaenopsis nurseriesfrom the central and southern Taiwan. Aphelenchoides could be found in up to 35 Phalaenopsis greenhouses among 48 surveyed, the radio was about 72%. Three Aphelenchoides population ABC, AU, AL were collected in this investigation. All of them had the typical characteristics of genus Aphelenchoides under the optical microscope. The nematode body was linear, with the stylet and the stylet knob, three parts of esophagus, the width of the median esophageal bulb was more than 75% of the body width. Female had single-ovary with the posterior uterine branch. Based on external morphology and measurement of De Man's formula could determine the ABC isolate to be Aphelenchoides bicaudatus, the AU and AL were similar to Aphelenchoides composticola. The region of 18S rDNA of three Aphelenchoides isolates, compared with the Genebank database system, confirmed ABC isolate was Aphelenchoides bicaudatus. AU and AL have 99% similarity, but their sequences were not similar to any of species data in Genebank, however, they shares no similarity with the 4 major Aphelenchoides species. Three Aphelenchoides isolates (AU, AL, ABC) could successfully infect orchid by inoculating 1000 nematodes on roots by pricking wounds. The nematodes and eggs could be observed in the root 1 month later, which revealed that these nematodes could complete their life cycle in the roots. Three Aphelenchoides populations isolated from orchid could penetrate to rice and strawberry. Inoculating Dendrobium miyakei Schlt., Asplenium nidus L., Saintpaulia ionantha H. and Cyclamen persicum Mill. showed no symptoms, and no nematodes were re-isolated from the inoculation sites. Carbofuran, Phenamiphos and DCIP had better control effect compared to 5 other commonly used nematicide tested, and also showed the least phytotoxicity to orchids.
URI: http://hdl.handle.net/11455/31453
其他識別: U0005-2308201016083800
Appears in Collections:植物病理學系

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