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Separation of gluconic acid from trehalose by ion exchange adsorption
|關鍵字:||葡萄糖酸;gluconic acid;海藻糖;離子交換樹脂;吸附;trehalose;ion exchange;adsorption||出版社:||化學工程學系所||引用:|| Schiraldi C, Di Lernia I, De Rosa M. Trehalose production: exploiting novel approaches. TRENDS in Biotechnology 2002;20:420-5.  Brennan PJ, Nikaido H. The Envelope of Mycobacteria. Annual Review of Biochemistry 1995;64:29-63.  Puech V, Chami M, Lemassu A, Laneelle M-A, Schiffler B, Gounon P, et al. Structure of the cell envelope of corynebacteria: importance of the non-covalently bound lipids in the formation of the cell wall permeability barrier and fracture plane. Microbiology 2001;147:1365-82.  Goddijn OJ, van Dun K. Trehalose metabolism in plants. Trends in plant science 1999;4:315-9.  Eastmond PJ, Graham IA. Trehalose metabolism: a regulatory role for trehalose-6-phosphate? Current opinion in plant biology 2003;6:231-5.  Chen Q, Behar KL, Xu T, Fan C, Haddad GG. Expression of Drosophila trehalose-phosphate synthase in HEK-293 cells increases hypoxia tolerance. 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本研究以陰離子交換樹脂Amberlite IRA67以及Diaion PA316去做離子的交換，將葡萄糖酸吸附於樹脂上，進而將其移出海藻糖水溶液中，達到純化二者的效果。然後對陰離子交換樹脂進行探討，探討其吸附動力以及吸附熱力學，以找出最適化吸附條件。Amberlite IRA67在水溶液於低pH值時吸附過程符Langmuir Isotherm，進而求出最大吸附量q以及K：分別為2.2618(mmole/g)、0.0267(mmole/L)。再由穿透曲線得知貫穿點時間約為250min，吸附飽和時間約為475min.，而475min.時貫穿濃度為73. 066mM，最後再由平衡曲線以及貫穿曲線算出ka值11.23min.-1，用以提供放大生產時之參數。而經由脫附曲線可以算出吸附後的陰離子交換樹脂，再以脫附劑500mM NaCl脫附回收葡萄糖酸，回收率大概介於85~95%。Diaion PA316在水溶液於pH值7時吸附過程並不符合Langmuir Isotherm。
In recent years, trehalose and gluconic acid are promises to be popular in foodstuff、cosmetology and pharmaceutical industry. It has stability, moisture resistance and low sweetness. With the increase of the trehalose and gluconic acid demand, the need to explore alternative feedstock sources and purification processes that are inexpensive and efficient is becoming more important. Then there have enzyme immobilization method to produces trehalose and gluconic acid from maltose .
This paper reports the purification results of trehalose and gluconic acid by using weak anion exchanger Amberlite IRA-67 and Diaion PA316. Then, explore the anion exchange resin adsorption dynamics and thermodynamics, and then find optimal adsorption conditions. Adsorption isotherm and breakthrough curves for the separation of trehalose and gluconic acid were obtained at pH 2.4 and 7.0, respectively. About the Amberlite IRA67,the isotherm was found to be a Langmuir type at pH 2.4. At pH 2.4, the maximum adsorption capacity of the resin, q and dissociation constant, K were 2.2618(mmole/gwet resin) and 0.0276(mmole/L), respectively. Breakthough time and exhaustion time have been found by breakthrough curve of trehalose and gluconic acid； tB：250min. and tE：475min. And concentration at exhaustion time is 73. 066mM .
Finally ,we found the constant ka (11.23min.-1 )by using equilibrium curve and breakthrough curve,it can be use at big production in the company. When column separation was performed at pH 2.5 by using desorbent (500mM NaCl), the total yield was about 85~95%. Diaion PA316 the isotherm was found not to be Langmuir type at pH 7 .
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