Please use this identifier to cite or link to this item:
標題: 應用深層液態培養義大利白松露(Tuber magnatum)生產總三萜-不同添加劑效應之探討
Production of Triterpenoids by Tuber magnatum in Submerged-effect of various additions
作者: 許祐誠
Hsu, Yu-Cheng
關鍵字: 液態醱酵;Tuber magnatum;三萜類;幾丁聚醣;亞麻油酸;fermentation;triterpenoids;chitosan;linoleic acid
出版社: 化學工程學系所
引用: 1. Mello, A., C. Murat, and P. Bonfante, Truffles: much more than a prized and local fungal delicacy. FEMS Microbiology Letters, 2006. 260(1): p. 1-8. 2. Tang, Y., et al., Comparison of Lipid Content and Fatty Acid Composition between Tuber Fermentation Mycelia and Natural Fruiting Bodies. Journal of Agricultural and Food Chemistry, 2011. 59(9): p. 4736-4742. 3. Wang, G., et al., Determination of 5α-androst-16-en-3α-ol in truffle fermentation broth by solid-phase extraction coupled with gas chromatography–flame ionization detector/electron impact mass spectrometry. Journal of Chromatography B, 2008. 870(2): p. 209-215. 4. 水野卓、川合正允, 菇類的化學‧生物化學1997: 國立編譯館. 5. Surenjav, U., et al., Effects of molecular structure on antitumor activities of (1→3)-β-d-glucans from different Lentinus Edodes. Carbohydrate Polymers, 2006. 63(1): p. 97-104. 6. 高曉薇, 樟芝對倉鼠體內脂質代謝與抗氧化狀態之影響1992: 台北輔仁大學碩士論文. 7. Kawagishi, H., Katsumi, R., Sazawa, T., Mizuno, T., Hagiwara, T. and and T. Nakamura, Cytotoxic Steroids from the Mushroom Agaricus blazei. Pytochemistry, 1989. 27: p. 2777. 8. Mizuno, M., Morimoto, M., Minate, K. and Tsuchida, H, Polysaccharides from Agaricus blazei Stimulate Lymphocyte T-Cell Subsets in Mice. Bioscience, Biotechnology, and Biochemistry, 1998. 62: p. 434. 9. Gurfinkel, D.M. and A.V. Rao, Determination of Saponins in Legumes by Direct Densitometry. Journal of Agricultural and Food Chemistry, 2001. 50(3): p. 426-430. 10. Wasser SP, W.A., Medicinal properties of substances occurring in Higher Basidiomycetes mushrooms: current perspectives. International Journal of Medicinal Mushrooms, 1999. 1: p. 31-62. 11. Itoh, K., et al., The interplay of interleukin-10 (IL-10) and interleukin-2 (IL-2) in humoral immune responses: IL-10 synergizes with IL-2 to enhance responses of human B lymphocytes in a mechanism which is different from upregulation of CD25 expression. Cell Immunol, 1994. 157(2): p. 478-88. 12. Liu, D., et al., Maternal Care, Hippocampal Glucocorticoid Receptors, and Hypothalamic-Pituitary-Adrenal Responses to Stress. Science, 1997. 277(5332): p. 1659-1662. 13. Mizuno, T., et al., Antitumor Activity and Some Properties of Water-soluble Polysaccharides from Himematsutake Fruiting Body of Agaricm blazei Murill. Agricultural and Biological Chemistry, 1990. 54(11): p. 2889-2896. 14. Moradali, M.-F., et al., Immunomodulating and anticancer agents in the realm of macromycetes fungi macrofungi. International Immunopharmacology, 2007. 7(6): p. 701-724. 15. 科學人, 松露的秘密生活2010 . 05. 70-75. 16. 王祥初, 松露-豬從地下拱出的真菌 食物探源, 2012. 17. 葉瓊文, 應用義大利白松露 Tube magnatum 生產多醣體最適化培養之探討論文2010: 中興大學化學工程學研究所碩士論文. 18. 黃建閎, 世界頂級珍饈美饌-松露. 2011. 19. 胡弘道, 與林木共生的珍貴食材食用真菌. 科學發展, 2005.5. 389. 20. 邢薇, 天堂的饕餮白松露. 烹飪原料, 2010. 21. Lai, A., 白松露:破土而出的馥郁. 美味追尋, 2010. 22. Humfeld, H., The Production of Mushroom Mycelium Agaricus camestris in submerged culture. Science, 1948. 107: p. 373 -378. 23. 王培銘, 食藥用菇液態培養製程之開發. 食品工業, 2002. 34: p. 31-35. 24. 徐晴、黄和、李霜、張昆、付永前、何皓、高振, 深層醱酵中絲狀真菌菌球型態控制的策略. 食品科技, 2009. 34(2). 25. Metz, B., Kossen, N.W.F., The growth of molds in the form of pellets. Biotechnology and Bioengineering, 1977. 19: p. 781-799. 26. Woodruff, H.B., Natural products from microorganisms. Science, 1980. 208: p. 1225-1229. 27. 蕭明熙, 真菌代謝之最近研究趨勢. 真菌學之最新發展"專題演講論文集專刊第12號, 1985: p. 168-183. 28. 王聲遠, 開發真菌次級代謝物為醫藥品. Fungi Science, 2001. 16: p. 1-6. 29. 肖崇厚、陳蘊如, 中藥化學1989, 上海: 科學技術出版社. 323–360. 30. 李宛蓁, 樟芝菌絲體培養與生理活性成分生成之研究2003: 東海大學化學工程學研究所碩士論文. 31. Manitto, Biosynthesis of Natural products1981. 319. 32. 王伯徹、黃仁彰, 靈芝與樟芝之研發與市場面面觀. 食品工業, 2002. 34(5): p. 3-17. 33. 黃惠君, 食用藥菇的營養與藥用價值. 食品工業, 2004. 36(5): p. 25-32. 34. 顏國欽, 樟芝菌絲體液體培養萃取物抗氧化及抗腫瘤特性之探討. 92年度「保健食品研究開發」計畫成果發表會, 2003: p. 5-13. 35. Ikeda, Y., A. Murakami, and H. Ohigashi, Ursolic acid: an anti- and pro-inflammatory triterpenoid. Molecular Nutrition & Food Research, 2008. 52(1): p. 26-42. 36. Huang, Y., et al., Effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf on inflammatory cytokine and mediator induction from alveolar macrophages of chronic bronchitic rats. Journal of Inflammation Research, 2007. 56(2): p. 76-82. 37. Nangia-Makker, P., et al., Inhibition of breast tumor growth and angiogenesis by a medicinal herb: Ocimum gratissimum. International Journal of Cancer 2007. 121(4): p. 884-94. 38. Slamenova, D., et al., Reduction of DNA-damaging effects of anti-HIV drug 3''-azido-3''-dideoxythymidine on human cells by ursolic acid and lignin biopolymer. Neoplasma, 2006. 53(6): p. 485-91. 39. Yu, D., et al., Anti-AIDS agents 69. Moronic acid and other triterpene derivatives as novel potent anti-HIV agents. Journal of Medicinal Chemistry, 2006. 49(18): p. 5462-9. 40. Kim, K.A., et al., Inhibition of cytochrome P450 activities by oleanolic acid and ursolic acid in human liver microsomes. Life Sciences, 2004. 74(22): p. 2769-79. 41. Ovesna, Z., et al., Pentacyclic triterpenoic acids: new chemoprotective compounds. Minireview. Neoplasma, 2004. 51(5): p. 327-33. 42. Yang, L., et al., 23,24-Dihydrocucurbitacin B induces G2/M cell-cycle arrest and mitochondria-dependent apoptosis in human breast cancer cells (Bcap37). Cancer Letters, 2007. 256(2): p. 267-278. 43. Tian, Z., et al., Anti-hepatoma activity and mechanism of ursolic acid and its derivatives isolated from Aralia decaisneana. World Journal of Gastroenterology, 2006. 12(6): p. 874-9. 44. Hsu, Y.L., et al., Asiatic acid, a triterpene, induces apoptosis and cell cycle arrest through activation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways in human breast cancer cells. Journal of Pharmacology and Experimental Therapeutics, 2005. 313(1): p. 333-44. 45. 姚强、高興喜、宫志遠、孔明、任鵬飛, 不同脂肪酸對靈芝液體醱酵的影響. 食用菌學報, 2010. 17(3): p. 55-59. 46. 姚强、高興喜、宫志遠、孔明、任鵬飛, 不同脂肪酸對桑黄液體醱酵的影響. 食品科學, 2011. 32(7): p. 198-201. 47. Liu, C.-J., C.-C. Chiang, and B.-H. Chiang, The elicited two-stage submerged cultivation of Antrodia cinnamomea for enhancing triterpenoids production and antitumor activity. Biochemical Engineering Journal, 2012. 64(0): p. 48-54. 48. Zhao, J., L.C. Davis, and R. Verpoorte, Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances, 2005. 23(4): p. 283-333. 49. Ravi Kumar, M.N.V., A review of chitin and chitosan applications. Reactive and Functional Polymers, 2000. 46(1): p. 1-27. 50. 林庚申、張小青、魏鐵錚、郭良棟, 樺褐孔菌三萜類物質的提取與含量測定. 菌物學報, 2007. 26(3): p. 396~403. 51. 姚松君、黄生權、陳壯耀、曾慶孝, 超音波輔助提取靈芝三萜的工藝研究. Modern Food Science and Technology, 2009. 25(10): p. 1220-1223. 52. Tang, Y.-J., et al., Significance of inoculation density and carbon source on the mycelial growth and Tuber polysaccharides production by submerged fermentation of Chinese truffle Tuber sinense. Process Biochemistry, 2008. 43(5): p. 576-586. 53. Patel, G.B. and B.J. Agnew, Growth and butyric acid production by Clostridium populeti. Archives of Microbiology, 1988. 150(3): p. 267-271. 54. Fang, Q.-H. and J.-J. Zhong, Effect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum. Process Biochemistry, 2002. 37(7): p. 769-774. 55. Lee, I.H., Chen, C. T., Chen, H. C., Hsu, W. C., Sugar flux in response to carbohydrate-feeding of cultured Antrodia camphorata, a recently described medicinal fungus in Taiwan. Chinese Medical Journal, 2002. 13(1): p. 21-31. 56. Jonathan, S.G. and I.O. Fasidi, Studies on phytohormones, vitamins and mineral element requirements of Lentinus subnudus (Berk) and schizophyllum commune (Fr. Ex. Fr) from Nigeria. Food Chemistry, 2001. 75(3): p. 303-307. 57. Seviour, R.J.a.B.K., Effect of ammonium ion concentration on polysaccharide production by Aureobasidium pullulans in batch culture. European Journal of Applied Microbiology and Biotechnology, 1983. 17: p. 178-181. 58. Eilert, U., F. Constabel, and W.G.W. Kurz, Elicitor-stimulation of Monoterpene Indole Alkaloid Formation in Suspension Cultures of Catharanthus roseus. Journal of Plant Physiology, 1986. 126(1): p. 11-22. 59. Prakash, G. and A.K. Srivastava, Statistical elicitor optimization studies for the enhancement of azadirachtin production in bioreactor Azadirachta indica cell cultivation. Biochemical Engineering Journal, 2008. 40(2): p. 218-226. 60. Wang, G., et al., Determination of 5alpha-androst-16-en-3alpha-ol in truffle fermentation broth by solid-phase extraction coupled with gas chromatography-flame ionization detector/electron impact mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci, 2008. 870(2): p. 209-15.
本研究是以義大利白松露 Tuber magnatum 開發液態醱酵生產菌絲體和總三萜類,在研究成果得知,不同的碳、氮源對菌絲體及代謝物的生產之影響極大。在不同碳氮源測試實驗中,以sucrose為最佳碳源濃度為10 g/L,其總三萜類含量為132.26±1.77 mg/L,而氮源部分以yeast extract為最佳氮源濃度15 g/L,其總三萜類含量為157.68±14.33 mg/L。
為了提升總三萜量,添加不同的濃度的亞麻油酸、棕櫚酸、硬脂酸、幾丁聚醣、氯化鈣和樟木萃取液,而探討其影響。實驗發現,以2g/L亞麻油酸和125mg/L幾丁聚醣產總三萜量為最多,分別為327.49±42.15 mg/L及343.16±9.25 mg/L ,約略提升了2.17倍。最後以義大利白松露子實體與菌絲體進行氣相層析質譜儀的分析,初步分析結果,皆含有玉蕊醇,而菌絲體含有脫氫異雄固酮。
其他識別: U0005-1508201214340300
Appears in Collections:化學工程學系所

Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.