Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3218
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dc.contributor劉永銓zh_TW
dc.contributorYung-Chuan Liuen_US
dc.contributor.author許祐誠zh_TW
dc.contributor.authorHsu, Yu-Chengen_US
dc.contributor.other化學工程學系所zh_TW
dc.date2012en_US
dc.date.accessioned2014-06-06T05:31:28Z-
dc.date.available2014-06-06T05:31:28Z-
dc.identifierU0005-1508201214340300en_US
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dc.identifier.urihttp://hdl.handle.net/11455/3218-
dc.description.abstract近年來,廣泛地利用液態發酵培養食用菇類生產菌體和其代謝產物,已經成為食用菇類的一種趨勢。而食用菇類最吸引人的地方,在於它的代謝產物能夠抗腫瘤活性、保肝作用等多種醫療作用。 本研究是以義大利白松露 Tuber magnatum 開發液態醱酵生產菌絲體和總三萜類,在研究成果得知,不同的碳、氮源對菌絲體及代謝物的生產之影響極大。在不同碳氮源測試實驗中,以sucrose為最佳碳源濃度為10 g/L,其總三萜類含量為132.26±1.77 mg/L,而氮源部分以yeast extract為最佳氮源濃度15 g/L,其總三萜類含量為157.68±14.33 mg/L。 為了提升總三萜量,添加不同的濃度的亞麻油酸、棕櫚酸、硬脂酸、幾丁聚醣、氯化鈣和樟木萃取液,而探討其影響。實驗發現,以2g/L亞麻油酸和125mg/L幾丁聚醣產總三萜量為最多,分別為327.49±42.15 mg/L及343.16±9.25 mg/L ,約略提升了2.17倍。最後以義大利白松露子實體與菌絲體進行氣相層析質譜儀的分析,初步分析結果,皆含有玉蕊醇,而菌絲體含有脫氫異雄固酮。zh_TW
dc.description.tableofcontents摘要 III ABSTRACT II 圖目錄……………………………………………………………………………….. iii 表目錄 VIII 第一章、 緒論 1 1.1前言 1 1.2研究動機 2 第二章、 文獻回顧 3 2.1 義大利白松露菌(TUBER MAGNATUM) 3 2.1.1 白松露菌之簡介 5 2.1.2 白松露子實體成長環境和培養方法 5 2.1.3 白松露菌絲體在液態醱酵中的型態 10 2.2 三萜類( TRITERPENOIDS ) 13 2.2.1萜類的介紹 13 2.2.2 三萜類之生合成機制 14 2.2.3 三萜類的分類 17 2.2.4 三萜類的療效 20 2.3 誘導劑(ELICITOR) 21 2.3.1 脂肪酸 21 2.3.2 添加物 21 第三章、 實驗材料與方法 23 3.1 實驗藥品 23 3.2 實驗設備 24 3.3 實驗菌種 25 3.4 培養基組成 25 3.4.1 固態平面菌株培養 25 3.4.2 液態種瓶培養 25 3.4.3 搖瓶培養之試驗 27 3.5 實驗分析 29 3.5.1 實驗流程 29 3.5.2 乾菌重分析 30 3.5.3 殘留糖濃度的測定 30 3.5.4 總三萜類含量測定 31 3.5.5 氣相層析質譜儀(GC-MS)分析條件 32 第四章、 結果與討論 33 4.1 超音波輔助萃取總三萜 33 4.1.1 萃取溶劑之影響 33 4.1.2 萃取體積之影響 35 4.1.3 萃取時間之影響 36 4.2 培養基之搖瓶試驗 37 4.2.1 義大利白松露菌絲體之生長曲線 37 4.2.2 碳源之搖瓶試驗 39 4.2.3 碳源濃度影響 41 4.2.4 氮源之搖瓶試驗 43 4.2.5 氮源濃度影響 45 4.2.6 添加物添加影響 47 4.3氣相層析質譜儀(GC-MS)分析 55 第五章、 結論與未來展望 59 參考文獻 61 附錄 65zh_TW
dc.language.isozh_TWen_US
dc.publisher化學工程學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1508201214340300en_US
dc.subject液態醱酵zh_TW
dc.subjectTuber magnatumen_US
dc.subject三萜類zh_TW
dc.subject幾丁聚醣zh_TW
dc.subject亞麻油酸zh_TW
dc.subjectfermentationen_US
dc.subjecttriterpenoidsen_US
dc.subjectchitosanen_US
dc.subjectlinoleic aciden_US
dc.title應用深層液態培養義大利白松露(Tuber magnatum)生產總三萜-不同添加劑效應之探討zh_TW
dc.titleProduction of Triterpenoids by Tuber magnatum in Submerged-effect of various additionsen_US
dc.typeThesis and Dissertationzh_TW
item.languageiso639-1zh_TW-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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