Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32665
DC FieldValueLanguage
dc.contributor.authorHsiao, H.J.en_US
dc.contributor.author王之仰zh_TW
dc.contributor.authorLiu, P.A.en_US
dc.contributor.authorYeh, H.I.en_US
dc.contributor.authorWang, C.Y.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T07:44:04Z-
dc.date.available2014-06-06T07:44:04Z-
dc.identifier.issn0304-8608zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32665-
dc.description.abstractClassical swine fever is a contagious disease of pigs characterized by fatal hemorrhagic fever. Classical swine fever virus (CSFV) induces the expression of pro-inflammatory and pro-coagulant factors of vascular endothelial cells and establishes a long-term infection. This study aimed to understand the effect of CSFV on endothelial connexin 43 (Cx43) expression and gap junctional intercellular coupling (GJIC). Porcine aortic endothelial cells were infected with CSFV at different multiplicity of infection for 48 h. Semi-quantitative RT-PCR, immunoconfocal microscopy, and Western blotting showed that the transcription and translation of Cx43 were reduced, and this was associated with an attenuation of GJIC. This decrease occurred in a time-dependent manner. An ERK inhibitor (PD98059), a JNK inhibitor (SP600125), and proteasome/lysosome inhibitors all significantly reversed the reduction in Cx43 protein levels without any influence on the titer of progeny virus. In addition, CSFV activated ERK and JNK in a time-dependent manner and down-regulated Cx43 promoter activity, mainly through decreased AP2 binding. This effect was primarily caused by the replication of CSFV rather than a consequence of cytokines being induced by CSFV infection of endothelial cells.en_US
dc.language.isoen_USzh_TW
dc.relationArchives of Virologyen_US
dc.relation.ispartofseriesArchives of Virology, Volume 155, Issue 7, Page(s) 1107-1116.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s00705-010-0693-1en_US
dc.subjectnf-kappa-ben_US
dc.subjectin-vitroen_US
dc.subjectintercellular communicationen_US
dc.subjectepithelial-cellsen_US
dc.subjectexpressionen_US
dc.subjectphosphorylationen_US
dc.subjectproteinen_US
dc.subjectdegradationen_US
dc.subjectinfectionen_US
dc.subjectmechanismen_US
dc.titleClassical swine fever virus down-regulates endothelial connexin 43 gap junctionsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s00705-010-0693-1zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
Appears in Collections:獸醫學系所
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