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標題: A new detection technique for fluoroquinolone-conjugated proteins by high performance liquid chromatography with UV/fluorescence detectors
作者: Hung, S.W.
Shih, C.W.
Chen, B.R.
Tu, C.Y.
Ling, Y.F.
Tsou, L.T.
Ho, S.P.
Wang, W.S.
關鍵字: fluoroquinolone;HPLC;N-hydroxysuccinimide ester;bovine serum albumin
Project: Journal of Food and Drug Analysis
期刊/報告no:: Journal of Food and Drug Analysis, Volume 15, Issue 1, Page(s) 71-74.
This study was aimed to develop a simple, fast, and reliable technique to detect fluoroquinolones (FQs)-conjugated bovine serum albumin (BSA). Four tested FQs, enrofloxacin, ofloxacin, danofloxacin, and orbifloxacin, were conjugated with BSA by following the N-hydroxysuccinimide ester method. The technique was designed according to the different absorption characteristics of the FQs and BSA; FQs can be detected by both UV and fluorescence detectors, but BSA can only be detected by UV. The results demonstrated that the developed method was efficient in detecting FQs-BSA conjugates. In addition, the method not only trace the FQs and BSA conjugation responses but also can be used to estimate the level of FQs-BSA conjugation. Therefore, this technique is a valuable toot for the detection drug-carrier-conjugated antigens, especially for FQs-BSA-conjugates during the production of anti-FQs monoclonal antibodies.
ISSN: 1021-9498
Appears in Collections:獸醫學系所

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