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標題: Combination of G-CSF Administration and Human Amniotic Fluid Mesenchymal Stem Cell Transplantation Promotes Peripheral Nerve Regeneration
作者: Pan, H.C.
Chen, C.J.
Cheng, F.C.
Ho, S.P.
Liu, M.J.
Hwang, S.M.
Chang, M.H.
Wang, Y.C.
關鍵字: Apoptosis;Amniotic fluid mesenchymal stem cells;G-CSF;Sciatic nerve;injury;Inflammatory cytokines;colony-stimulating factor;spinal-cord-injury;focal cerebral-ischemia;sciatic-nerve;enhanced regeneration;functional recovery;in-vitro;alpha;rat;death
Project: Neurochemical Research
期刊/報告no:: Neurochemical Research, Volume 34, Issue 3, Page(s) 518-527.
Amniotic fluid mesenchymal stem cells (AFS) harbor the potential to improve peripheral nerve injury by inherited neurotrophic factor secretion, but present the drawback of the short-term survival after transplantation. Granulocyte-colony stimulating factor (G-CSF) has a diversity of functions, including anti-inflammatory and anti-apoptotic effects. This study was conducted to evaluate whether G-CSF could augment the neuroprotective effect of transplanted AFS against peripheral nerve injury. The potential involvement of anti-inflammation/anti-apoptosis effect was also investigated. Peripheral nerve injury was produced in Sprauge-Dawley rats by crushing left sciatic nerve using a vessel clamp. The AFS were embedded in fibrin glue and delivered to the injured site. G-CSF (50 mu g/kg) was administrated by intra-peritoneal injection for 7 consecutive days. Cell apoptosis, inflammatory cytokines, motor function, and nerve regeneration were evaluated 7 or 28 days after injury. Crush injury induced inflammatory response, disrupted nerve integrity, and impaired nerve function in sciatic nerve. Crush injury-provoked inflammation was attenuated in groups receiving G-CSF but not in AFS only group. In transplanted AFS, marked apoptosis was detected and this event was reduced by G-CSF treatment. Increased nerve myelination and improved motor function were observed in AFS transplanted, G-CSF administrated, and AFS/G-CSF combined treatment groups. Significantly, the combined treatment showed the most beneficial effect. In conclusion, the concomitant treatment of AFS with G-CSF augments peripheral nerve regeneration which may involve the suppression of apoptotic death in implanted AFS and the attenuation of inflammatory response.
ISSN: 0364-3190
DOI: 10.1007/s11064-008-9815-5
Appears in Collections:獸醫學系所

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