Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32702
DC FieldValueLanguage
dc.contributor.authorPan, H.C.en_US
dc.contributor.author何素鵬zh_TW
dc.contributor.authorChin, C.S.en_US
dc.contributor.authorYang, D.Y.en_US
dc.contributor.authorHo, S.P.en_US
dc.contributor.authorChen, C.J.en_US
dc.contributor.authorHwang, S.M.en_US
dc.contributor.authorChang, M.H.en_US
dc.contributor.authorCheng, F.C.en_US
dc.date2009zh_TW
dc.date.accessioned2014-06-06T07:44:07Z-
dc.date.available2014-06-06T07:44:07Z-
dc.identifier.issn0364-3190zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32702-
dc.description.abstractPurpose Attenuation of pro-inflammatory cytokines and associated inflammatory cell deposits rescues human amniotic fluid mesenchymal stem cells (AFS) from apoptosis. Hyperbaric oxygen (HBO) suppressed stimulus-induced pro-inflammatory cytokine production in blood-derived monocyte-macrophages. Herein, we evaluate the beneficial effect of hyperbaric oxygen on transplanted AFS in a sciatic nerve injury model. Methods Peripheral nerve injury was produced in Sprague-Dawley rats by crushing the left sciatic nerve using a vessel clamp. The AFS were embedded in fibrin glue and delivered to the injured site. Hyperbaric oxygen (100% oxygen, 2 ATA, 60 min/day) was administered 12 h after operation for seven consecutive days. Transplanted cell apoptosis, oxidative stress, inflammatory cell deposits and associated chemokines, pro-inflammatory cytokines, motor function, and nerve regeneration were evaluated 7 and 28 days after injury. Results Crush injury induced an inflammatory response, disrupted nerve integrity, and impaired nerve function in the sciatic nerve. However, crush injury-provoked inflammatory cytokines, deposits of inflammatory cytokines, and associated macrophage migration chemokines were attenuated in groups receiving hyperbaric oxygen but not in the AFS-only group. No significant increase in oxidative stress was observed after administration of HBO. In transplanted AFS, marked apoptosis was detected and this event was reduced by HBO treatment. Increased nerve myelination and improved motor function were observed in AFS-transplant, HBO-administrated, and AFS/HBO-combined treatment groups. Significantly, the AFS/HBO combined treatment showed the most beneficial effect. Conclusion AFS in combination with HBO augment peripheral nerve regeneration, which may involve the suppression of apoptotic death in implanted AFS and the attenuation of an inflammatory response detrimental to peripheral nerve regeneration.en_US
dc.language.isoen_USzh_TW
dc.relationNeurochemical Researchen_US
dc.relation.ispartofseriesNeurochemical Research, Volume 34, Issue 7, Page(s) 1304-1316.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s11064-008-9910-7en_US
dc.subjectApoptosisen_US
dc.subjectAmniotic fluid mesenchymal stem cellsen_US
dc.subjectHyperbaric oxygenen_US
dc.subjectSciatic nerve injuryen_US
dc.subjectInflammatory cytokinesen_US
dc.subjectrat sciatic-nerveen_US
dc.subjectnecrosis-factor-alphaen_US
dc.subjecttnf-alphaen_US
dc.subjectin-vitroen_US
dc.subjectwallerianen_US
dc.subjectdegenerationen_US
dc.subjectschwann-cellsen_US
dc.subjectexposureen_US
dc.subjectinjuryen_US
dc.subjecttherapyen_US
dc.subjectmacrophagesen_US
dc.titleHuman Amniotic Fluid Mesenchymal Stem Cells in Combination with Hyperbaric Oxygen Augment Peripheral Nerve Regenerationen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s11064-008-9910-7zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
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