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標題: Effect of thuringiensin on adenylate cyclase in rat cerebral cortex
作者: Tsai, S.F.
Yang, C.
Wang, S.C.
Wang, J.S.
Hwang, J.S.
Ho, S.P.
關鍵字: thuringiensin;cyclic adenosine monophosphate (cAMP);adenylate cyclase;forskolin;cerebral cortex;bacillus-thuringiensis;forskolin;inhibition;exotoxin;cells;isoforms;brain;accumulation;activation;membranes
Project: Toxicology and Applied Pharmacology
期刊/報告no:: Toxicology and Applied Pharmacology, Volume 194, Issue 1, Page(s) 34-40.
The purpose of this work is to evaluate the effect of thuringiensin on the adenylate cyclase activity in rat cerebral cortex. The cyclic adenosine 3' 5' -monophosphate (cAMP) levels were shown to be dose-dependently elevated 17-450% or 54-377% by thuringiensin at concentrations of 10 muM-100 mM or 0.5-4 mM, due to the activation of basal adenylate cyclase activity of rat cerebral cortical membrane preparation. Thuringiensin also activated basal activity of a commercial adenylate cyclase from Escherichia coli. However, the forskolin-stimulated adenylate cyclase activity in rat cerebral cortex was inhibited by thuringiensin at concentrations of 1-100 muM, thus cAMP production decreased. Furthermore, thuringiensin or adenylate cyclase inhibitor (MDL-12330A) reduced the forskolin (10 muM)-stimulated adenylate cyclase activity at concentrations of 10 muM, 49% or 43% inhibition, respectively. In conclusion, this study demonstrated that thuringiensin could activate basal adenylate cyclase activity and increase cAMP concentrations in rat cerebral cortex or in a commercial adenylate cyclase. Comparing the dose-dependent effects of thuringiensin on the basal and forskolin-stimulated adenylate cyclase activity, thuringiensin can be regarded as a weak activator of adenylate cyclase or an inhibitor of forskolin-stimulated adenylate cyclase. (C) 2003 Elsevier Inc. All rights reserved.
ISSN: 0041-008X
DOI: 10.1016/j.taap.2003.08.015
Appears in Collections:獸醫學系所

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