Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32774
標題: The effect of different extenders on post-thaw sperm survival, acrosomal integrity and longevity in cryopreserved semen of Formosan Sika deer and Formosan Sambar deer
作者: Cheng, F.P.
董光中
Wu, J.T.
Chan, J.P.W.
Wang, J.S.
Fung, H.P.
Colenbrander, B.
Tung, K.C.
陳鵬文
鄭豐邦
關鍵字: Formosan Sika deer;Formosan Sambar deer;semen cryopreservation;frozen-thawed spermatozoa;fallow deer;artificial-insemination;boar;spermatozoa;cervus-elaphus;dama-dama;egg-yolk;intrauterine;insemination;stallion spermatozoa;peanut agglutinin
Project: Theriogenology
期刊/報告no:: Theriogenology, Volume 61, Issue 9, Page(s) 1605-1616.
摘要: 
This study investigates the efficacy of five extenders in contributing to the outcome of semen cryopreservation in Formosan Sika and Sambar deer. Pooled semen (n = 4) of six males of each breed was used. In Sika deer, semen collection rate was 96% (23/24) over all electro-ejaculations. Volume, sperm motility and sperm concentration of fresh ejaculates was 0.5 +/- 0.4 ml, 77 +/- 6% and 1471.3 +/- 940.0 x 10(6) ml(-1), respectively. Post-thaw motility in respective extender was A: 66 +/- 16%; B: 71 +/- 2%; C: 73 +/- 6%; D: 9 +/- 4% and E: 26 +/- 12% (mean +/- S.D.). In extender C (74 +/- 14%) more viable spermatozoa were preserved than in the others (A: 64 +/- 10%; B: 48 +/- 11%; D: 41 +/- 16%; E: 47 +/- 6%; P < 0.05). Acrosomal integrity was not influenced by extender composition. Post-thaw motility did not decrease during a 4-h incubation period, irrespective of the extender used (P > 0.05). In Sambar deer, semen collection rate was 88% (21/24) over all electro-ejaculations. Volume, sperm motility and sperm concentration of fresh ejaculates was 1.3 +/- 0.5 ml, 82 +/- 4% and 379.1 +/- 252.2 x 10(6) ml(-1), respectively. Post-thaw motility was in respective extenders A: 69 +/- 2%; B: 74 +/- 6%; C: 73 +/- 2%; D: 13 +/- 6% and E: 31 +/- 120%. Extenders B and C were superior (P > 0.05) with respect to sperm motility. Similarly, post-thaw viability in extenders A (70 +/- 7%), B (76 +/- 7%) and C (79 +/- 2%) was higher than that D (25 +/- 19%) and E (29 +/- 17%) (P < 0.01). Sperm acrosomal integrity was better preserved in extenders B (86 +/- 4%) and C (83 +/- 4%) than in extenders A (54 +/- 13%), D (39 +/- 22%) and E (46 +/- 22%) (P < 0.05). Post-thaw sperm longevity in extender A reduced from 69 to 16% during incubation (P < 0.05) whereas only a slight decrease was observed in the other extenders after 4 h. In conclusion these data show that eggyolk-Tris-Tes-glycerol based extender C containing Equex STM paste is optimal for freezing semen of Formosan Sika deer while eggyolk-Tris--citric acid-glycerol based extender B containing Equex and extender C are superior in semen cryopreservation to others for Formosan Sambar deer. (C) 2003 Published by Elsevier Inc.
URI: http://hdl.handle.net/11455/32774
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2003.07.015
Appears in Collections:獸醫學系所

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