Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32826
DC FieldValueLanguage
dc.contributor.authorWong, M.L.en_US
dc.contributor.author張天傑zh_TW
dc.contributor.authorLiu, J.J.en_US
dc.contributor.authorChang, Y.S.en_US
dc.contributor.authorChang, T.J.en_US
dc.date1998zh_TW
dc.date.accessioned2014-06-06T07:44:16Z-
dc.date.available2014-06-06T07:44:16Z-
dc.identifier.issn0916-7250zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32826-
dc.description.abstractThe glycoprotein E2 sequences of classical swine fever virus (strain p97) were cloned, sequenced and expressed in E. coli. Result from SDS-polyacrylamide gel electrophoresis analysis of expressed proteins revealed the presence of a prominently stained band corresponding to a molecular mass of 61 kDa, which is in agreement with the predicted size from the DNA sequence. The recombinant E2 protein contained an aminoterminal tag of six histidines that could be used for purification by the nickel chelate affinity chromatography. The elution fractions of the expressed protein also contain additional bands of 40 and 35 kDa proteins, indicating proteolytic cleavages might occur. Our Western blotting result also supported that the expression of the recombinant E2 protein of the classical swine fever virus were accomplished.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Veterinary Medical Scienceen_US
dc.relation.ispartofseriesJournal of Veterinary Medical Science, Volume 60, Issue 4, Page(s) 541-544.en_US
dc.relation.urihttp://dx.doi.org/10.1292/jvms.60.541en_US
dc.subjectclassical swine fever virusen_US
dc.subjectglycoprotein E2en_US
dc.subjecthog-cholera virusen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectmolecular-cloningen_US
dc.subjectstrainen_US
dc.subjectbresciaen_US
dc.subjectcdnaen_US
dc.subjectpestivirusesen_US
dc.subjectpigsen_US
dc.subjectrnaen_US
dc.subjecte1en_US
dc.titleExpression of the glycoprotein E2 of the classical swine fever virus in Escherichia colien_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1292/jvms.60.541zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
Appears in Collections:獸醫學系所
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